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Conference Paper: Preliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam
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TitlePreliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam
 
AuthorsBaudon, EM
Trevennec, K
Peiris, JSM
Roger, F
Garcia, J
 
Issue Date2011
 
PublisherInstitut Pasteur International Network.
 
CitationThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 47, abstract no. P02 [How to Cite?]
 
AbstractBackground: To study the pig-poultry interspecies transmission, the French Agricultural Research Center for International Development (CIRAD) carried out a cross-sectional survey in pig and poultry farms and a longitudinal study in a local pig slaughterhouse in North Vietnam from April 2009 to February 2010. The preliminary laboratory results of this study are presented here. A screening of sera by a commercial competitive enzyme-linked immunosorbent assay (cELISA) for influenza A was done in Vietnam, and further serological analyses on cELISA positive sera and virological analyses were performed in the Pasteur Research Centre in Hong Kong. Results: A total of 2340 pigs were sampled in farms and 678 in slaughterhouse, with 22.3% positive by cELISA in farms, and 37.2% in slaughterhouse; 1554 poultry (ducks and chickens) were sampled in farms, with 30.4% positive by cELISA. In Hong Kong, 710 pigs and 37 poultry swabs were analyzed by isolation in cell culture and quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 677 pigs and 472 poultry cELISA positive sera by hemagglutination inhibition (HI) tests and H5 pseudoparticle-based seroneutralization assay (H5pp). No virus was isolated nor detected by qRT-PCR in swabs from pigs and poultry. Among pigs, 13.3% of sera in farms and 17.6% in slaughterhouse were positive for at least one strain tested, 19.1% for poultry sera. According to the considered subsets, 5.5% to 11.3% of sera were positive by cELISA but remained negative by HI test and H5pp assay for the tested strains. Pig sera were tested positive against strains of H1N1 (12.1% in farms, 17.1% in slaughterhouse), H9N2 (1.7% in farms, 1.2% in slaughterhouse), and H5N1 (0.7% in farms, 0.1% in slaughterhouse), HI tests did not reveal evidence of infection by a H3 subtype. For poultry, some sera were positive against H5N1 (11.8%) and H9N2 (9.7%) strains, but no positive serum against H6 strains was detected. Sera in pigs and poultry were found to cross-react between several subtypes and strains within a subtype. Conclusion: Several strains were found in both pigs and poultry sera, suggesting the occurrence of inter-species transmission. The interpretation of analyses can be challenging due to the lack of information on the viral strains circulating in field and to the potential cross-reaction between different strains. Further analyses are carried out in order to identify the possible circulation of other strains and to evaluate the performance of the cELISA.
 
DescriptionPoster Presentation
The Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf
 
DC FieldValue
dc.contributor.authorBaudon, EM
 
dc.contributor.authorTrevennec, K
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorRoger, F
 
dc.contributor.authorGarcia, J
 
dc.date.accessioned2011-09-23T06:28:27Z
 
dc.date.available2011-09-23T06:28:27Z
 
dc.date.issued2011
 
dc.description.abstractBackground: To study the pig-poultry interspecies transmission, the French Agricultural Research Center for International Development (CIRAD) carried out a cross-sectional survey in pig and poultry farms and a longitudinal study in a local pig slaughterhouse in North Vietnam from April 2009 to February 2010. The preliminary laboratory results of this study are presented here. A screening of sera by a commercial competitive enzyme-linked immunosorbent assay (cELISA) for influenza A was done in Vietnam, and further serological analyses on cELISA positive sera and virological analyses were performed in the Pasteur Research Centre in Hong Kong. Results: A total of 2340 pigs were sampled in farms and 678 in slaughterhouse, with 22.3% positive by cELISA in farms, and 37.2% in slaughterhouse; 1554 poultry (ducks and chickens) were sampled in farms, with 30.4% positive by cELISA. In Hong Kong, 710 pigs and 37 poultry swabs were analyzed by isolation in cell culture and quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 677 pigs and 472 poultry cELISA positive sera by hemagglutination inhibition (HI) tests and H5 pseudoparticle-based seroneutralization assay (H5pp). No virus was isolated nor detected by qRT-PCR in swabs from pigs and poultry. Among pigs, 13.3% of sera in farms and 17.6% in slaughterhouse were positive for at least one strain tested, 19.1% for poultry sera. According to the considered subsets, 5.5% to 11.3% of sera were positive by cELISA but remained negative by HI test and H5pp assay for the tested strains. Pig sera were tested positive against strains of H1N1 (12.1% in farms, 17.1% in slaughterhouse), H9N2 (1.7% in farms, 1.2% in slaughterhouse), and H5N1 (0.7% in farms, 0.1% in slaughterhouse), HI tests did not reveal evidence of infection by a H3 subtype. For poultry, some sera were positive against H5N1 (11.8%) and H9N2 (9.7%) strains, but no positive serum against H6 strains was detected. Sera in pigs and poultry were found to cross-react between several subtypes and strains within a subtype. Conclusion: Several strains were found in both pigs and poultry sera, suggesting the occurrence of inter-species transmission. The interpretation of analyses can be challenging due to the lack of information on the viral strains circulating in field and to the potential cross-reaction between different strains. Further analyses are carried out in order to identify the possible circulation of other strains and to evaluate the performance of the cELISA.
 
dc.descriptionPoster Presentation
 
dc.descriptionThe Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf
 
dc.identifier.citationThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 47, abstract no. P02 [How to Cite?]
 
dc.identifier.epage47, abstract no. P02
 
dc.identifier.hkuros194084
 
dc.identifier.spage47, abstract no. P02
 
dc.identifier.urihttp://hdl.handle.net/10722/141232
 
dc.languageeng
 
dc.publisherInstitut Pasteur International Network.
 
dc.relation.ispartofThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases'
 
dc.titlePreliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam
 
dc.typeConference_Paper
 
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<item><contributor.author>Baudon, EM</contributor.author>
<contributor.author>Trevennec, K</contributor.author>
<contributor.author>Peiris, JSM</contributor.author>
<contributor.author>Roger, F</contributor.author>
<contributor.author>Garcia, J</contributor.author>
<date.accessioned>2011-09-23T06:28:27Z</date.accessioned>
<date.available>2011-09-23T06:28:27Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>The International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;, Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;, 2011, p. 47, abstract no. P02</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/141232</identifier.uri>
<description>Poster Presentation</description>
<description>The Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf</description>
<description.abstract>Background: To study the pig-poultry interspecies transmission, the French Agricultural Research 
Center for International Development (CIRAD) carried out a cross-sectional survey in pig and 
poultry farms and a longitudinal study in a local pig slaughterhouse in North Vietnam from April 
2009 to February 2010. The preliminary laboratory results of this study are presented here. A 
screening of sera by a commercial competitive enzyme-linked immunosorbent assay (cELISA) for 
influenza A was done in Vietnam, and further serological analyses on cELISA positive sera and 
virological analyses were performed in the Pasteur Research Centre in Hong Kong.
Results: A total of 2340 pigs were sampled in farms and 678 in slaughterhouse, with 22.3% 
positive by cELISA in farms, and 37.2% in slaughterhouse; 1554 poultry (ducks and chickens) were 
sampled in farms, with 30.4% positive by cELISA. In Hong Kong, 710 pigs and 37 poultry swabs 
were analyzed by isolation in cell culture and quantitative reverse transcription polymerase chain 
reaction (qRT-PCR), and 677 pigs and 472 poultry cELISA positive sera by hemagglutination 
inhibition (HI) tests and H5 pseudoparticle-based seroneutralization assay (H5pp). No virus was 
isolated nor detected by qRT-PCR in swabs from pigs and poultry. Among pigs, 13.3% of sera in 
farms and 17.6% in slaughterhouse were positive for at least one strain tested, 19.1% for poultry 
sera. According to the considered subsets, 5.5% to 11.3% of sera were positive by cELISA but 
remained negative by HI test and H5pp assay for the tested strains. Pig sera were tested positive 
against strains of H1N1 (12.1% in farms, 17.1% in slaughterhouse), H9N2 (1.7% in farms, 1.2% in 
slaughterhouse), and H5N1 (0.7% in farms, 0.1% in slaughterhouse), HI tests did not reveal 
evidence of infection by a H3 subtype. For poultry, some sera were positive against H5N1 (11.8%) 
and H9N2 (9.7%) strains, but no positive serum against H6 strains was detected. Sera in pigs and 
poultry were found to cross-react between several subtypes and strains within a subtype.
Conclusion: Several strains were found in both pigs and poultry sera, suggesting the occurrence of 
inter-species transmission. The interpretation of analyses can be challenging due to the lack of 
information on the viral strains circulating in field and to the potential cross-reaction between 
different strains. Further analyses are carried out in order to identify the possible circulation of 
other strains and to evaluate the performance of the cELISA.</description.abstract>
<language>eng</language>
<publisher>Institut Pasteur International Network.</publisher>
<relation.ispartof>The International Symposium on &apos;Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases&apos;</relation.ispartof>
<title>Preliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam</title>
<type>Conference_Paper</type>
<identifier.hkuros>194084</identifier.hkuros>
<identifier.spage>47, abstract no. P02</identifier.spage>
<identifier.epage>47, abstract no. P02</identifier.epage>
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