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Conference Paper: Preliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam

TitlePreliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnam
Authors
Issue Date2011
PublisherInstitut Pasteur International Network.
Citation
The International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 47, abstract no. P02 How to Cite?
Abstract
Background: To study the pig-poultry interspecies transmission, the French Agricultural Research Center for International Development (CIRAD) carried out a cross-sectional survey in pig and poultry farms and a longitudinal study in a local pig slaughterhouse in North Vietnam from April 2009 to February 2010. The preliminary laboratory results of this study are presented here. A screening of sera by a commercial competitive enzyme-linked immunosorbent assay (cELISA) for influenza A was done in Vietnam, and further serological analyses on cELISA positive sera and virological analyses were performed in the Pasteur Research Centre in Hong Kong. Results: A total of 2340 pigs were sampled in farms and 678 in slaughterhouse, with 22.3% positive by cELISA in farms, and 37.2% in slaughterhouse; 1554 poultry (ducks and chickens) were sampled in farms, with 30.4% positive by cELISA. In Hong Kong, 710 pigs and 37 poultry swabs were analyzed by isolation in cell culture and quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 677 pigs and 472 poultry cELISA positive sera by hemagglutination inhibition (HI) tests and H5 pseudoparticle-based seroneutralization assay (H5pp). No virus was isolated nor detected by qRT-PCR in swabs from pigs and poultry. Among pigs, 13.3% of sera in farms and 17.6% in slaughterhouse were positive for at least one strain tested, 19.1% for poultry sera. According to the considered subsets, 5.5% to 11.3% of sera were positive by cELISA but remained negative by HI test and H5pp assay for the tested strains. Pig sera were tested positive against strains of H1N1 (12.1% in farms, 17.1% in slaughterhouse), H9N2 (1.7% in farms, 1.2% in slaughterhouse), and H5N1 (0.7% in farms, 0.1% in slaughterhouse), HI tests did not reveal evidence of infection by a H3 subtype. For poultry, some sera were positive against H5N1 (11.8%) and H9N2 (9.7%) strains, but no positive serum against H6 strains was detected. Sera in pigs and poultry were found to cross-react between several subtypes and strains within a subtype. Conclusion: Several strains were found in both pigs and poultry sera, suggesting the occurrence of inter-species transmission. The interpretation of analyses can be challenging due to the lack of information on the viral strains circulating in field and to the potential cross-reaction between different strains. Further analyses are carried out in order to identify the possible circulation of other strains and to evaluate the performance of the cELISA.
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/141232

 

DC FieldValueLanguage
dc.contributor.authorBaudon, EMen_US
dc.contributor.authorTrevennec, Ken_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorRoger, Fen_US
dc.contributor.authorGarcia, Jen_US
dc.date.accessioned2011-09-23T06:28:27Z-
dc.date.available2011-09-23T06:28:27Z-
dc.date.issued2011en_US
dc.identifier.citationThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', Phnom Penh, Cambodia, 29-31 May 2011. In the Program of the International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases', 2011, p. 47, abstract no. P02en_US
dc.identifier.urihttp://hdl.handle.net/10722/141232-
dc.descriptionPoster Presentation-
dc.descriptionThe Program can be viewed at: http://pasteur-network-meeting.org/KH2011/Downloads/PROGRAMME_FINAL.pdf-
dc.description.abstractBackground: To study the pig-poultry interspecies transmission, the French Agricultural Research Center for International Development (CIRAD) carried out a cross-sectional survey in pig and poultry farms and a longitudinal study in a local pig slaughterhouse in North Vietnam from April 2009 to February 2010. The preliminary laboratory results of this study are presented here. A screening of sera by a commercial competitive enzyme-linked immunosorbent assay (cELISA) for influenza A was done in Vietnam, and further serological analyses on cELISA positive sera and virological analyses were performed in the Pasteur Research Centre in Hong Kong. Results: A total of 2340 pigs were sampled in farms and 678 in slaughterhouse, with 22.3% positive by cELISA in farms, and 37.2% in slaughterhouse; 1554 poultry (ducks and chickens) were sampled in farms, with 30.4% positive by cELISA. In Hong Kong, 710 pigs and 37 poultry swabs were analyzed by isolation in cell culture and quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 677 pigs and 472 poultry cELISA positive sera by hemagglutination inhibition (HI) tests and H5 pseudoparticle-based seroneutralization assay (H5pp). No virus was isolated nor detected by qRT-PCR in swabs from pigs and poultry. Among pigs, 13.3% of sera in farms and 17.6% in slaughterhouse were positive for at least one strain tested, 19.1% for poultry sera. According to the considered subsets, 5.5% to 11.3% of sera were positive by cELISA but remained negative by HI test and H5pp assay for the tested strains. Pig sera were tested positive against strains of H1N1 (12.1% in farms, 17.1% in slaughterhouse), H9N2 (1.7% in farms, 1.2% in slaughterhouse), and H5N1 (0.7% in farms, 0.1% in slaughterhouse), HI tests did not reveal evidence of infection by a H3 subtype. For poultry, some sera were positive against H5N1 (11.8%) and H9N2 (9.7%) strains, but no positive serum against H6 strains was detected. Sera in pigs and poultry were found to cross-react between several subtypes and strains within a subtype. Conclusion: Several strains were found in both pigs and poultry sera, suggesting the occurrence of inter-species transmission. The interpretation of analyses can be challenging due to the lack of information on the viral strains circulating in field and to the potential cross-reaction between different strains. Further analyses are carried out in order to identify the possible circulation of other strains and to evaluate the performance of the cELISA.-
dc.languageengen_US
dc.publisherInstitut Pasteur International Network.-
dc.relation.ispartofThe International Symposium on 'Surveillance and Discovery in Respiratory and Other Emerging Infectious Diseases'en_US
dc.titlePreliminary laboratory results of an epidemiological study on avian and swine influenza in Vietnamen_US
dc.typeConference_Paperen_US
dc.identifier.emailBaudon, EM: ebaudon@hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.emailGarcia, J: jmgarcia@hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.identifier.hkuros194084en_US
dc.identifier.spage47, abstract no. P02-
dc.identifier.epage47, abstract no. P02-

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