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Article: Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae)

TitlePurification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae)
Authors
KeywordsIsolation
Laccase
Mushroom
Pleurotus cornucopiae
Issue Date2010
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm
Citation
Protein And Peptide Letters, 2010, v. 17 n. 8, p. 1040-1047 How to Cite?
AbstractA 66-kDa laccase, with an N-terminal sequence different from those of other mushroom laccases, was purified from fresh fruiting bodies of the edible mushroom Pleurotus cornucopiae by using affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. The procedure resulted in a 16-fold purification and a specific enzyme activity of 17.3 U mg -1. The optimum pH and temperature for the purified laccase were pH 4 and 40°C, respectively. This laccase inhibited proliferation of murine leukemia cell line L1210 and human hepatoma cell line HepG2, and reduced the activity of HIV-1 reverse transcriptase with an IC 50 of 22 μM. There was neither mitogenic activity toward mouse splenocytes, nor hemagglutinating/hemolytic activity toward rabbit erythrocytes. This study yielded information about the potentially exploitable activities of P. cornucopiae laccase. © 2010 Bentham Science Publishers Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/141038
ISSN
2015 Impact Factor: 1.069
2015 SCImago Journal Rankings: 0.472
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, JHen_HK
dc.contributor.authorNg, TBen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorLiu, Fen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorZhang, KYen_HK
dc.date.accessioned2011-09-23T06:24:07Z-
dc.date.available2011-09-23T06:24:07Z-
dc.date.issued2010en_HK
dc.identifier.citationProtein And Peptide Letters, 2010, v. 17 n. 8, p. 1040-1047en_HK
dc.identifier.issn0929-8665en_HK
dc.identifier.urihttp://hdl.handle.net/10722/141038-
dc.description.abstractA 66-kDa laccase, with an N-terminal sequence different from those of other mushroom laccases, was purified from fresh fruiting bodies of the edible mushroom Pleurotus cornucopiae by using affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. The procedure resulted in a 16-fold purification and a specific enzyme activity of 17.3 U mg -1. The optimum pH and temperature for the purified laccase were pH 4 and 40°C, respectively. This laccase inhibited proliferation of murine leukemia cell line L1210 and human hepatoma cell line HepG2, and reduced the activity of HIV-1 reverse transcriptase with an IC 50 of 22 μM. There was neither mitogenic activity toward mouse splenocytes, nor hemagglutinating/hemolytic activity toward rabbit erythrocytes. This study yielded information about the potentially exploitable activities of P. cornucopiae laccase. © 2010 Bentham Science Publishers Ltd.en_HK
dc.languageengen_US
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htmen_HK
dc.relation.ispartofProtein and Peptide Lettersen_HK
dc.subjectIsolationen_HK
dc.subjectLaccaseen_HK
dc.subjectMushroomen_HK
dc.subjectPleurotus cornucopiaeen_HK
dc.subject.meshHIV Reverse Transcriptase - antagonists and inhibitors-
dc.subject.meshHIV-1 - drug effects - enzymology-
dc.subject.meshLaccase - chemistry - isolation and purification - metabolism - pharmacology-
dc.subject.meshPleurotus - enzymology-
dc.subject.meshReverse Transcriptase Inhibitors - isolation and purification - metabolism - pharmacology-
dc.titlePurification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0929-8665&volume=17&issue=8&spage=1040&epage=1047&date=2010&atitle=Purification+and+characterization+of+a+laccase+with+inhibitory+activity+toward+HIV-1+reverse+transcriptase+and+tumor+cells+from+an+edible+mushroom+pleurotus+cornucopiae-
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailZhang, KY: ybzhang@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityZhang, KY=rp01410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2174/092986610791498966en_HK
dc.identifier.pmid19807674-
dc.identifier.scopuseid_2-s2.0-77958065455en_HK
dc.identifier.hkuros192433en_US
dc.identifier.hkuros171307-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77958065455&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1040en_HK
dc.identifier.epage1047en_HK
dc.identifier.isiWOS:000281419200012-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWong, JH=34874358200en_HK
dc.identifier.scopusauthoridNg, TB=35311803300en_HK
dc.identifier.scopusauthoridJiang, Y=7404833192en_HK
dc.identifier.scopusauthoridLiu, F=36062739500en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridZhang, KY=23483121900en_HK
dc.identifier.citeulike7403835-

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