File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.2174/092986610791498966
- Scopus: eid_2-s2.0-77958065455
- PMID: 19807674
- WOS: WOS:000281419200012
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae)
Title | Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae) |
---|---|
Authors | |
Keywords | Isolation Laccase Mushroom Pleurotus cornucopiae |
Issue Date | 2010 |
Publisher | Bentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm |
Citation | Protein And Peptide Letters, 2010, v. 17 n. 8, p. 1040-1047 How to Cite? |
Abstract | A 66-kDa laccase, with an N-terminal sequence different from those of other mushroom laccases, was purified from fresh fruiting bodies of the edible mushroom Pleurotus cornucopiae by using affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. The procedure resulted in a 16-fold purification and a specific enzyme activity of 17.3 U mg -1. The optimum pH and temperature for the purified laccase were pH 4 and 40°C, respectively. This laccase inhibited proliferation of murine leukemia cell line L1210 and human hepatoma cell line HepG2, and reduced the activity of HIV-1 reverse transcriptase with an IC 50 of 22 μM. There was neither mitogenic activity toward mouse splenocytes, nor hemagglutinating/hemolytic activity toward rabbit erythrocytes. This study yielded information about the potentially exploitable activities of P. cornucopiae laccase. © 2010 Bentham Science Publishers Ltd. |
Persistent Identifier | http://hdl.handle.net/10722/141038 |
ISSN | 2023 Impact Factor: 1.0 2023 SCImago Journal Rankings: 0.349 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, JH | en_HK |
dc.contributor.author | Ng, TB | en_HK |
dc.contributor.author | Jiang, Y | en_HK |
dc.contributor.author | Liu, F | en_HK |
dc.contributor.author | Sze, SCW | en_HK |
dc.contributor.author | Zhang, KY | en_HK |
dc.date.accessioned | 2011-09-23T06:24:07Z | - |
dc.date.available | 2011-09-23T06:24:07Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Protein And Peptide Letters, 2010, v. 17 n. 8, p. 1040-1047 | en_HK |
dc.identifier.issn | 0929-8665 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/141038 | - |
dc.description.abstract | A 66-kDa laccase, with an N-terminal sequence different from those of other mushroom laccases, was purified from fresh fruiting bodies of the edible mushroom Pleurotus cornucopiae by using affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. The procedure resulted in a 16-fold purification and a specific enzyme activity of 17.3 U mg -1. The optimum pH and temperature for the purified laccase were pH 4 and 40°C, respectively. This laccase inhibited proliferation of murine leukemia cell line L1210 and human hepatoma cell line HepG2, and reduced the activity of HIV-1 reverse transcriptase with an IC 50 of 22 μM. There was neither mitogenic activity toward mouse splenocytes, nor hemagglutinating/hemolytic activity toward rabbit erythrocytes. This study yielded information about the potentially exploitable activities of P. cornucopiae laccase. © 2010 Bentham Science Publishers Ltd. | en_HK |
dc.language | eng | en_US |
dc.publisher | Bentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm | en_HK |
dc.relation.ispartof | Protein and Peptide Letters | en_HK |
dc.subject | Isolation | en_HK |
dc.subject | Laccase | en_HK |
dc.subject | Mushroom | en_HK |
dc.subject | Pleurotus cornucopiae | en_HK |
dc.subject.mesh | HIV Reverse Transcriptase - antagonists and inhibitors | - |
dc.subject.mesh | HIV-1 - drug effects - enzymology | - |
dc.subject.mesh | Laccase - chemistry - isolation and purification - metabolism - pharmacology | - |
dc.subject.mesh | Pleurotus - enzymology | - |
dc.subject.mesh | Reverse Transcriptase Inhibitors - isolation and purification - metabolism - pharmacology | - |
dc.title | Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (pleurotus cornucopiae) | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0929-8665&volume=17&issue=8&spage=1040&epage=1047&date=2010&atitle=Purification+and+characterization+of+a+laccase+with+inhibitory+activity+toward+HIV-1+reverse+transcriptase+and+tumor+cells+from+an+edible+mushroom+pleurotus+cornucopiae | - |
dc.identifier.email | Sze, SCW: stephens@hku.hk | en_HK |
dc.identifier.email | Zhang, KY: ybzhang@hku.hk | en_HK |
dc.identifier.authority | Sze, SCW=rp00514 | en_HK |
dc.identifier.authority | Zhang, KY=rp01410 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.2174/092986610791498966 | en_HK |
dc.identifier.pmid | 19807674 | - |
dc.identifier.scopus | eid_2-s2.0-77958065455 | en_HK |
dc.identifier.hkuros | 192433 | en_US |
dc.identifier.hkuros | 171307 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77958065455&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 17 | en_HK |
dc.identifier.issue | 8 | en_HK |
dc.identifier.spage | 1040 | en_HK |
dc.identifier.epage | 1047 | en_HK |
dc.identifier.isi | WOS:000281419200012 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Wong, JH=34874358200 | en_HK |
dc.identifier.scopusauthorid | Ng, TB=35311803300 | en_HK |
dc.identifier.scopusauthorid | Jiang, Y=7404833192 | en_HK |
dc.identifier.scopusauthorid | Liu, F=36062739500 | en_HK |
dc.identifier.scopusauthorid | Sze, SCW=23482617000 | en_HK |
dc.identifier.scopusauthorid | Zhang, KY=23483121900 | en_HK |
dc.identifier.citeulike | 7403835 | - |
dc.identifier.issnl | 0929-8665 | - |