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Article: A new ColE1-like plasmid group revealed by comparative analysis of the replication proficient fragments of Vibrionaceae plasmids

TitleA new ColE1-like plasmid group revealed by comparative analysis of the replication proficient fragments of Vibrionaceae plasmids
Authors
KeywordsCompatibility
Plasmids
Replication
Replication initiation
RNA
Vibrio
Issue Date2010
PublisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/
Citation
Journal Of Microbiology And Biotechnology, 2010, v. 20 n. 8, p. 1163-1178 How to Cite?
AbstractPlasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replicationparticipating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids. ©The Korean Society for Microbiology and Biotechnology.
Persistent Identifierhttp://hdl.handle.net/10722/140902
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 0.548
ISI Accession Number ID
Funding AgencyGrant Number
Environmental Toxicology Research Reserve Fund
University of Hong Kong
Funding Information:

This work was supported in part by the Environmental Toxicology Research Reserve Fund, The University of Hong Kong. We are grateful to Dr. Patricia A. Sobecky and Dr. Tracy H. Hazen for support in the hybridization analysis of part of the plasmid DNA samples. We thank Jessie Lai and Eric Lee for technical assistance in the laboratories.

References

 

DC FieldValueLanguage
dc.contributor.authorPan, Len_HK
dc.contributor.authorLeung, PCen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2011-09-23T06:21:15Z-
dc.date.available2011-09-23T06:21:15Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Microbiology And Biotechnology, 2010, v. 20 n. 8, p. 1163-1178en_HK
dc.identifier.issn1017-7825en_HK
dc.identifier.urihttp://hdl.handle.net/10722/140902-
dc.description.abstractPlasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replicationparticipating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids. ©The Korean Society for Microbiology and Biotechnology.en_HK
dc.languageengen_US
dc.publisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/en_HK
dc.relation.ispartofJournal of Microbiology and Biotechnologyen_HK
dc.subjectCompatibilityen_HK
dc.subjectPlasmidsen_HK
dc.subjectReplicationen_HK
dc.subjectReplication initiationen_HK
dc.subjectRNAen_HK
dc.subjectVibrioen_HK
dc.subject.meshDNA Replication-
dc.subject.meshNucleic Acid Conformation-
dc.subject.meshPlasmids - chemistry - genetics-
dc.subject.meshReplication Origin-
dc.subject.meshVibrionaceae - chemistry - genetics-
dc.titleA new ColE1-like plasmid group revealed by comparative analysis of the replication proficient fragments of Vibrionaceae plasmidsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1017-7825&volume=20&issue=8&spage=1163&epage=1178&date=2010&atitle=A+new+ColE1-like+plasmid+group+revealed+by+comparative+analysis+of+the+replication+proficient+fragments+of+Vibrionaceae+plasmids-
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.4014/jmb.1003.03007en_HK
dc.identifier.pmid20798577-
dc.identifier.scopuseid_2-s2.0-77957088557en_HK
dc.identifier.hkuros194835en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77957088557&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1163en_HK
dc.identifier.epage1178en_HK
dc.identifier.isiWOS:000281405200001-
dc.publisher.placeKorea, Republic ofen_HK
dc.identifier.scopusauthoridPan, L=54393873900en_HK
dc.identifier.scopusauthoridLeung, PC=12782513900en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK

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