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Conference Paper: Cobalt chloride pre-treatment enhances the calcium handling of human embryonic stem cells-derived cardiomyocytes

TitleCobalt chloride pre-treatment enhances the calcium handling of human embryonic stem cells-derived cardiomyocytes
Authors
KeywordsBiology
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717204/description#description
Citation
The 16th International Conference on the International Society of Differentiation, Nara, Japan, 15–18 November 2010. In Differentiation, 2010, v. 80 suppl. 1, p. S44, abstract P81 How to Cite?
AbstractHypoxia is an important factor governing the proliferation, differentiation and maintenance of the cardiovascular system during embryonic development. At a molecular level, hypoxia inducible factor-1 (HIF-1) plays a pivotal role in handling the hypoxia signal. Our previous study has demonstrated the direct involvement of HIF-1α subunit in promoting the cardiac differentiation of murine embryonic stem cells (ESCs). Here, we report an alternative experimental approach of using cobalt chloride to induce HIF-1α stabilization in the human ESCs for promoting cardiac differentiation. In brief, treatment of undifferentiated hES2 human ESCs with 50 μM cobalt chloride remarkably increased the protein levels of the HIF-1α subunit, which was associated with increased expressions of early cardiac specific transcription factors and cardiotrophic factors including NK2 transcription factor related locus 5, vascular endothelial growth factor and cardiotrophin-1. When the pre-treated cells were subjected to cardiac differentiation, notable increase in the occurrence of beating embryoid bodies, along with increased expressions of the cardiac specific markers, such as myosin heavy (MHC-A and MHC-B) and light (MLC2V) chains were observed. Confocal calcium imaging analysis further indicated that the maximum upstroke and decay velocities were significantly increased in both non-caffeine and caffeine-induced calcium transient in cardiomyocytes derived from the cobalt chloride pre-treated cells, suggesting these cells were functionally more mature. In conclusion, the findings from the present study suggested that cobalt chloride pretreatment can enhance the maturation of calcium handling of cardiomyocytes derived from ESC for therapeutic applications.
DescriptionThis journal suppl. contain abstracts of the The 16th International Conference on the International Society of Differentiation ... 2010.
Persistent Identifierhttp://hdl.handle.net/10722/140560
ISSN
2015 Impact Factor: 2.461
2015 SCImago Journal Rankings: 1.747
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, KMen_US
dc.contributor.authorChan, YCen_US
dc.contributor.authorLee, YKen_US
dc.contributor.authorLai, WHen_US
dc.contributor.authorFung, MLen_US
dc.contributor.authorSiu, CWen_US
dc.contributor.authorLi, RAen_US
dc.contributor.authorTse, HFen_US
dc.date.accessioned2011-09-23T06:14:23Z-
dc.date.available2011-09-23T06:14:23Z-
dc.date.issued2010en_US
dc.identifier.citationThe 16th International Conference on the International Society of Differentiation, Nara, Japan, 15–18 November 2010. In Differentiation, 2010, v. 80 suppl. 1, p. S44, abstract P81en_US
dc.identifier.issn0301-4681-
dc.identifier.urihttp://hdl.handle.net/10722/140560-
dc.descriptionThis journal suppl. contain abstracts of the The 16th International Conference on the International Society of Differentiation ... 2010.-
dc.description.abstractHypoxia is an important factor governing the proliferation, differentiation and maintenance of the cardiovascular system during embryonic development. At a molecular level, hypoxia inducible factor-1 (HIF-1) plays a pivotal role in handling the hypoxia signal. Our previous study has demonstrated the direct involvement of HIF-1α subunit in promoting the cardiac differentiation of murine embryonic stem cells (ESCs). Here, we report an alternative experimental approach of using cobalt chloride to induce HIF-1α stabilization in the human ESCs for promoting cardiac differentiation. In brief, treatment of undifferentiated hES2 human ESCs with 50 μM cobalt chloride remarkably increased the protein levels of the HIF-1α subunit, which was associated with increased expressions of early cardiac specific transcription factors and cardiotrophic factors including NK2 transcription factor related locus 5, vascular endothelial growth factor and cardiotrophin-1. When the pre-treated cells were subjected to cardiac differentiation, notable increase in the occurrence of beating embryoid bodies, along with increased expressions of the cardiac specific markers, such as myosin heavy (MHC-A and MHC-B) and light (MLC2V) chains were observed. Confocal calcium imaging analysis further indicated that the maximum upstroke and decay velocities were significantly increased in both non-caffeine and caffeine-induced calcium transient in cardiomyocytes derived from the cobalt chloride pre-treated cells, suggesting these cells were functionally more mature. In conclusion, the findings from the present study suggested that cobalt chloride pretreatment can enhance the maturation of calcium handling of cardiomyocytes derived from ESC for therapeutic applications.-
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717204/description#description-
dc.relation.ispartofDifferentiationen_US
dc.rightsDifferentiation. © Elsevier Ltd.-
dc.subjectBiology-
dc.titleCobalt chloride pre-treatment enhances the calcium handling of human embryonic stem cells-derived cardiomyocytesen_US
dc.typeConference_Paperen_US
dc.identifier.emailNg, KM: skykmng@hkucc.hku.hken_US
dc.identifier.emailChan, YC: yauchi@graduate.hku.hken_US
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_US
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hken_US
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hken_US
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_US
dc.identifier.authorityNg, KM=rp01670en_US
dc.identifier.authorityChan, YC=rp01502en_US
dc.identifier.authorityFung, ML=rp00433en_US
dc.identifier.authoritySiu, CW=rp00534en_US
dc.identifier.authorityLi, RA=rp01352en_US
dc.identifier.doi10.1016/j.diff.2010.09.087-
dc.identifier.hkuros195774en_US
dc.identifier.volume80-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS44, abstract P81-
dc.identifier.epageS44, abstract P81-
dc.identifier.isiWOS:000283589700119-
dc.publisher.placeUnited Kingdom-

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