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Conference Paper: 5-HT2A receptor mediated facilitation of inhibitory neurotransmission in medial vestibular nucleus

Title5-HT2A receptor mediated facilitation of inhibitory neurotransmission in medial vestibular nucleus
Authors
Keywordsserotonin
sIPSC
GABA
Issue Date2010
PublisherSociety for Neuroscience.
Citation
Neuroscience 2010, San Diego, CA., 13-17 November 2010. How to Cite?
AbstractSerotonergic projections from the raphe nucleus are known to innervate the medial vestibular nucleus (MVN). However, the role of this projection is yet to be determined. Whole-cell patch clamp technique was used to investigate serotonergic modulation of inhibitory neurotransmission on MVN neurons of rats. Perfusion of serotonin (5-HT) to brainstem slice containing the vestibular nucleus greatly increased the frequency and amplitude of spontaneous inhibitory postsynaptic currents of MVN neurons but did not change their rise time nor decay time. Administration of selective 5-HT2A receptor agonist could mimic the bursting effect of 5-HT and this was blocked by 5-HT2A receptor antagonist. Nevertheless, this enhancement of inhibitory transmission was not observed with the application of 5-HT1A, 5-HT1B or 5-HT3 receptor agonists. These demonstrate that inhibitory transmission within the MVN could be enhanced via 5-HT2A receptor but not other types of 5-HT receptors. Interestingly, this enhancement effect of 5-HT disappeared with the perfusion of tetrodoxin that blocked action potentials, suggesting that the effect of 5-HT on MVN neurons is mediated via presynaptic 5-HT receptors. Using GABAA receptor antagonist or glycine receptor antagonist, we further identified that both receptors took part in enhancing the inhibitory effects of 5-HT. Taken together, our results indicate that presynaptic 5-HT2A receptors function to facilitate both GABAA receptor- and glycine receptor-mediated inhibition of MVN neurons.
DescriptionPoster Session 543: Serotonin Receptors
Program#543.6 & Poster#: E24
Fulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2554&sKey=f3b43c95-3f52-433c-a478-872f308372be&cKey=be81ddaf-0609-4b5b-846d-454545b4888c&mKey=e5d5c83f-ce2d-4d71-9dd6-fc7231e090fb
Persistent Identifierhttp://hdl.handle.net/10722/140519

 

DC FieldValueLanguage
dc.contributor.authorHan, Len_US
dc.contributor.authorLai, SKen_US
dc.contributor.authorLai, CHen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2011-09-23T06:13:50Z-
dc.date.available2011-09-23T06:13:50Z-
dc.date.issued2010en_US
dc.identifier.citationNeuroscience 2010, San Diego, CA., 13-17 November 2010.en_US
dc.identifier.urihttp://hdl.handle.net/10722/140519-
dc.descriptionPoster Session 543: Serotonin Receptors-
dc.descriptionProgram#543.6 & Poster#: E24-
dc.descriptionFulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2554&sKey=f3b43c95-3f52-433c-a478-872f308372be&cKey=be81ddaf-0609-4b5b-846d-454545b4888c&mKey=e5d5c83f-ce2d-4d71-9dd6-fc7231e090fb-
dc.description.abstractSerotonergic projections from the raphe nucleus are known to innervate the medial vestibular nucleus (MVN). However, the role of this projection is yet to be determined. Whole-cell patch clamp technique was used to investigate serotonergic modulation of inhibitory neurotransmission on MVN neurons of rats. Perfusion of serotonin (5-HT) to brainstem slice containing the vestibular nucleus greatly increased the frequency and amplitude of spontaneous inhibitory postsynaptic currents of MVN neurons but did not change their rise time nor decay time. Administration of selective 5-HT2A receptor agonist could mimic the bursting effect of 5-HT and this was blocked by 5-HT2A receptor antagonist. Nevertheless, this enhancement of inhibitory transmission was not observed with the application of 5-HT1A, 5-HT1B or 5-HT3 receptor agonists. These demonstrate that inhibitory transmission within the MVN could be enhanced via 5-HT2A receptor but not other types of 5-HT receptors. Interestingly, this enhancement effect of 5-HT disappeared with the perfusion of tetrodoxin that blocked action potentials, suggesting that the effect of 5-HT on MVN neurons is mediated via presynaptic 5-HT receptors. Using GABAA receptor antagonist or glycine receptor antagonist, we further identified that both receptors took part in enhancing the inhibitory effects of 5-HT. Taken together, our results indicate that presynaptic 5-HT2A receptors function to facilitate both GABAA receptor- and glycine receptor-mediated inhibition of MVN neurons.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2010-
dc.rightsNeuroscience 2010. Copyright © Society for Neuroscience.-
dc.subjectserotonin-
dc.subjectsIPSC-
dc.subjectGABA-
dc.title5-HT2A receptor mediated facilitation of inhibitory neurotransmission in medial vestibular nucleusen_US
dc.typeConference_Paperen_US
dc.identifier.emailLai, SK: estherlai@hkusua.hku.hken_US
dc.identifier.emailLai, CH: chlaib@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.authorityLai, CH=rp00396en_US
dc.identifier.hkuros195360en_US
dc.publisher.placeUnited States-

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