File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: 3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs

Title3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
Authors
Keywords3D matrix adhesions
dynamic compression
human mesenchymal stem cells
Mechanotransduction
Issue Date2010
PublisherIEEE.
Citation
The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37 How to Cite?
AbstractThe current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/140396
ISBN
References

 

DC FieldValueLanguage
dc.contributor.authorLi, TCWen_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2011-09-23T06:10:53Z-
dc.date.available2011-09-23T06:10:53Z-
dc.date.issued2010en_HK
dc.identifier.citationThe IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37en_HK
dc.identifier.isbn978-1-61284-154-0-
dc.identifier.urihttp://hdl.handle.net/10722/140396-
dc.description.abstractThe current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.en_HK
dc.languageengen_US
dc.publisherIEEE.-
dc.relation.ispartofProceedings of the IEEE International Conference on Nano/Molecular Medicine and Engineeringen_HK
dc.rightsIEEE International Conference on Nano/Molecular Medicine and Engineering Proceedings. Copyright © IEEE.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rights©2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.-
dc.subject3D matrix adhesionsen_HK
dc.subjectdynamic compressionen_HK
dc.subjecthuman mesenchymal stem cellsen_HK
dc.subjectMechanotransductionen_HK
dc.title3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=978-1-61284-154-0&volume=&spage=34&epage=37&date=2010&atitle=3D+matrix+adhesions+mediating+mechanostranduction+in+hMSC-collagen+constructs-
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1109/NANOMED.2010.5749801en_HK
dc.identifier.scopuseid_2-s2.0-79955993522en_HK
dc.identifier.hkuros196491en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955993522&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.spage34en_HK
dc.identifier.epage37en_HK
dc.description.otherThe IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37-
dc.identifier.scopusauthoridLi, TCW=38861954700en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats