Conference Paper: 3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs

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Publisher Website: 10.1109/NANOMED.2010.5749801
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Title	3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
Authors	Li, TCW1 Chan, BP1
Keywords	3D matrix adhesions dynamic compression human mesenchymal stem cells Mechanotransduction
Issue Date	2010
Publisher	IEEE.
Citation	The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37 [How to Cite?] DOI: http://dx.doi.org/10.1109/NANOMED.2010.5749801
Abstract	The current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.
ISBN	978-1-61284-154-0
DOI	http://dx.doi.org/10.1109/NANOMED.2010.5749801
References	References in Scopus

DC Field	Value
dc.contributor.author	Li, TCW
dc.contributor.author	Chan, BP
dc.date.accessioned	2011-09-23T06:10:53Z
dc.date.available	2011-09-23T06:10:53Z
dc.date.issued	2010
dc.description.abstract	The current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.
dc.description.nature	published_or_final_version
dc.description.other	The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37
dc.identifier.citation	The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37 [How to Cite?] DOI: http://dx.doi.org/10.1109/NANOMED.2010.5749801
dc.identifier.doi	http://dx.doi.org/10.1109/NANOMED.2010.5749801
dc.identifier.epage	37
dc.identifier.hkuros	196491
dc.identifier.isbn	978-1-61284-154-0
dc.identifier.openurl
dc.identifier.scopus	eid_2-s2.0-79955993522
dc.identifier.spage	34
dc.identifier.uri	http://hdl.handle.net/10722/140396
dc.language	eng
dc.publisher	IEEE.
dc.relation.ispartof	Proceedings of the IEEE International Conference on Nano/Molecular Medicine and Engineering
dc.relation.references	References in Scopus
dc.rights	IEEE International Conference on Nano/Molecular Medicine and Engineering Proceedings. Copyright © IEEE.
dc.rights	Creative Commons: Attribution 3.0 Hong Kong License
dc.rights	©2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
dc.subject	3D matrix adhesions
dc.subject	dynamic compression
dc.subject	human mesenchymal stem cells
dc.subject	Mechanotransduction
dc.title	3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
dc.type	Conference_Paper

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Author Affiliations

The University of Hong Kong