File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Conference Paper: 3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
  • Basic View
  • Metadata View
  • XML View
Title3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
 
AuthorsLi, TCW1
Chan, BP1
 
Keywords3D matrix adhesions
dynamic compression
human mesenchymal stem cells
Mechanotransduction
 
Issue Date2010
 
PublisherIEEE.
 
CitationThe IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37 [How to Cite?]
DOI: http://dx.doi.org/10.1109/NANOMED.2010.5749801
 
AbstractThe current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.
 
ISBN978-1-61284-154-0
 
DOIhttp://dx.doi.org/10.1109/NANOMED.2010.5749801
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, TCW
 
dc.contributor.authorChan, BP
 
dc.date.accessioned2011-09-23T06:10:53Z
 
dc.date.available2011-09-23T06:10:53Z
 
dc.date.issued2010
 
dc.description.abstractThe current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α 5 β 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. © 2010 IEEE.
 
dc.description.naturepublished_or_final_version
 
dc.description.otherThe IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37
 
dc.identifier.citationThe IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37 [How to Cite?]
DOI: http://dx.doi.org/10.1109/NANOMED.2010.5749801
 
dc.identifier.doihttp://dx.doi.org/10.1109/NANOMED.2010.5749801
 
dc.identifier.epage37
 
dc.identifier.hkuros196491
 
dc.identifier.isbn978-1-61284-154-0
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-79955993522
 
dc.identifier.spage34
 
dc.identifier.urihttp://hdl.handle.net/10722/140396
 
dc.languageeng
 
dc.publisherIEEE.
 
dc.relation.ispartofProceedings of the IEEE International Conference on Nano/Molecular Medicine and Engineering
 
dc.relation.referencesReferences in Scopus
 
dc.rightsIEEE International Conference on Nano/Molecular Medicine and Engineering Proceedings. Copyright © IEEE.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.rights©2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.
 
dc.subject3D matrix adhesions
 
dc.subjectdynamic compression
 
dc.subjecthuman mesenchymal stem cells
 
dc.subjectMechanotransduction
 
dc.title3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs
 
dc.typeConference_Paper
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Li, TCW</contributor.author>
<contributor.author>Chan, BP</contributor.author>
<date.accessioned>2011-09-23T06:10:53Z</date.accessioned>
<date.available>2011-09-23T06:10:53Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37</identifier.citation>
<identifier.isbn>978-1-61284-154-0</identifier.isbn>
<identifier.uri>http://hdl.handle.net/10722/140396</identifier.uri>
<description.abstract>The current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin &#945; 5 &#946; 1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway. &#169; 2010 IEEE.</description.abstract>
<language>eng</language>
<publisher>IEEE.</publisher>
<relation.ispartof>Proceedings of the IEEE International Conference on Nano/Molecular Medicine and Engineering</relation.ispartof>
<rights>IEEE International Conference on Nano/Molecular Medicine and Engineering Proceedings. Copyright &#169; IEEE.</rights>
<rights>Creative Commons: Attribution 3.0 Hong Kong License</rights>
<rights>&#169;2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.</rights>
<subject>3D matrix adhesions</subject>
<subject>dynamic compression</subject>
<subject>human mesenchymal stem cells</subject>
<subject>Mechanotransduction</subject>
<title>3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs</title>
<type>Conference_Paper</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=978-1-61284-154-0&amp;volume=&amp;spage=34&amp;epage=37&amp;date=2010&amp;atitle=3D+matrix+adhesions+mediating+mechanostranduction+in+hMSC-collagen+constructs</identifier.openurl>
<description.nature>published_or_final_version</description.nature>
<identifier.doi>10.1109/NANOMED.2010.5749801</identifier.doi>
<identifier.scopus>eid_2-s2.0-79955993522</identifier.scopus>
<identifier.hkuros>196491</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-79955993522&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.spage>34</identifier.spage>
<identifier.epage>37</identifier.epage>
<description.other>The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 34-37</description.other>
<bitstream.url>http://hub.hku.hk/bitstream/10722/140396/1/Content.pdf</bitstream.url>
</item>
Author Affiliations
  1. The University of Hong Kong