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Conference Paper: Effect of dynamic mechanical compression on actin cytoskeleton network of human mesenchymal stem cells (hMSCs) in three dimensional collagen constructs
Title | Effect of dynamic mechanical compression on actin cytoskeleton network of human mesenchymal stem cells (hMSCs) in three dimensional collagen constructs |
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Authors | |
Keywords | Actin filament compression human mesenchymal stem cells Mechanoregulation |
Issue Date | 2010 |
Publisher | IEEE. |
Citation | The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 136-139 How to Cite? |
Abstract | Actin filament, one type of cytoskeletons, plays a central role in mediating cellular in responses to mechanical loading. Many mechanorgulation studies are restricted to 2D models using isolated cells or monolayer cultures, even though it is know that cells behave differently in term of cell morphology, cell matrix adhesion composition and matrix mediated force transmission when they are in 3D configuration. This current study investigates the temporal change of actin network of hMSCs entrapped in 3D collagen construct upon cyclic compression. Human bone marrow mesenchymal stem cells were encapsulated in cylindrical collagen construct. A micromanipulator based loading device coupled to fluorescent microscope was used to deliver compression loading to the construct with 10% strain at 1Hz for different period of time. Rhodamine phalloidin was used to stain for the actin filament network to hMSC in the construct at different time points postcompression. An optimized loading protocol with 5hrs of continuous loading was delivered. Actin network concentrated at the cell periphery of cells exhibiting round morphology was observed immediately while elongated and polarized actin network was found after 24 hours. Detailed characterization of actin filament organization and their association with cell-matrix interaction molecules are warrented before the mechanisms of compression-induced hMSC alignment can be delineated © 2010 IEEE. |
Persistent Identifier | http://hdl.handle.net/10722/140395 |
ISBN | |
References |
DC Field | Value | Language |
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dc.contributor.author | Ho, NFC | en_HK |
dc.contributor.author | Chan, BP | en_HK |
dc.date.accessioned | 2011-09-23T06:10:53Z | - |
dc.date.available | 2011-09-23T06:10:53Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 136-139 | en_HK |
dc.identifier.isbn | 978-1-61284-154-0 | - |
dc.identifier.uri | http://hdl.handle.net/10722/140395 | - |
dc.description.abstract | Actin filament, one type of cytoskeletons, plays a central role in mediating cellular in responses to mechanical loading. Many mechanorgulation studies are restricted to 2D models using isolated cells or monolayer cultures, even though it is know that cells behave differently in term of cell morphology, cell matrix adhesion composition and matrix mediated force transmission when they are in 3D configuration. This current study investigates the temporal change of actin network of hMSCs entrapped in 3D collagen construct upon cyclic compression. Human bone marrow mesenchymal stem cells were encapsulated in cylindrical collagen construct. A micromanipulator based loading device coupled to fluorescent microscope was used to deliver compression loading to the construct with 10% strain at 1Hz for different period of time. Rhodamine phalloidin was used to stain for the actin filament network to hMSC in the construct at different time points postcompression. An optimized loading protocol with 5hrs of continuous loading was delivered. Actin network concentrated at the cell periphery of cells exhibiting round morphology was observed immediately while elongated and polarized actin network was found after 24 hours. Detailed characterization of actin filament organization and their association with cell-matrix interaction molecules are warrented before the mechanisms of compression-induced hMSC alignment can be delineated © 2010 IEEE. | en_HK |
dc.language | eng | en_US |
dc.publisher | IEEE. | - |
dc.relation.ispartof | Proceedings of the IEEE International Conference on Nano/Molecular Medicine and Engineering | en_HK |
dc.rights | ©2010 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE. | - |
dc.subject | Actin filament | en_HK |
dc.subject | compression | en_HK |
dc.subject | human mesenchymal stem cells | en_HK |
dc.subject | Mechanoregulation | en_HK |
dc.title | Effect of dynamic mechanical compression on actin cytoskeleton network of human mesenchymal stem cells (hMSCs) in three dimensional collagen constructs | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=978-1-61284-154-0&volume=&spage=136&epage=139&date=2010&atitle=Effect+of+dynamic+mechanical+compression+on+actin+cytoskeleton+network+of+human+mesenchymal+stem+cells+(hMSCs)+in+three+dimensional+collagen+constructs | - |
dc.identifier.email | Chan, BP:bpchan@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chan, BP=rp00087 | en_HK |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1109/NANOMED.2010.5749820 | en_HK |
dc.identifier.scopus | eid_2-s2.0-79956008699 | en_HK |
dc.identifier.hkuros | 196490 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79956008699&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.spage | 136 | en_HK |
dc.identifier.epage | 139 | en_HK |
dc.description.other | The IEEE 4th International Conference on Nano/Molecular Medicine and Engineering (NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Proceedings of the IEEE International Conference on NANOMED, 2010, p. 136-139 | - |
dc.identifier.scopusauthorid | Ho, NFC=38861753600 | en_HK |
dc.identifier.scopusauthorid | Chan, BP=7201530390 | en_HK |