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Article: The beta-1-integrin-p-FAK-p130Cas-DOCK180-RhoA-vinculin is a novel regulatory protein complex at the apical ectoplasmic specialization in adult rat testes

TitleThe beta-1-integrin-p-FAK-p130Cas-DOCK180-RhoA-vinculin is a novel regulatory protein complex at the apical ectoplasmic specialization in adult rat testes
Authors
KeywordsTestis
Spermatogenesis
Apical ectoplasmic specialization
Adjudin
Seminiferous epithelial cycle
Issue Date2011
PublisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/spermatogenesis/
Citation
Spermatogenesis, 2011, v. 1 n. 1, p. 73-86 How to Cite?
AbstractDuring spermatogenesis, step 1 spermatids (round spermatids) derive from spermatocytes following meiosis I and II at stage XIV of the epithelial cycle begin a series of morphological transformation and differentiation via 19 steps in rats to form spermatozoa. This process is known as spermiogenesis, which is marked by condensation of the genetic material in the spermatid head, formation of the acrosome and elongation of the tail. Since developing spermatids are lacking the robust protein synthesis and transcriptional activity, the cellular, molecular and morphological changes associated with spermiogenesis rely on the Sertoli cell in the seminiferous epithelium via desmosome and gap junction between Sertoli cells and step 1-7 spermatids. Interestingly, a unique anchoring junction type arises at the interface of step 8 spermatid and Sertoli cell known as apical ectoplasmic specialization (apical ES). Once it appears, apical ES is the only anchoring device restricted to the interface of step 8-19 spermatids and Sertoli cells to confer spermatid polarity, adhesion, signal communication and structural support, and to provide nutritional support during spermiogenesis, replacing desmosome and gap junction. While the adhesion protein complexes that constitute the apical ES are known, the signaling protein complexes that regulate apical ES dynamics, however, remain largely unknown. Herein we report the presence of a FAK (focal adhesion kinase)-p130Cas (p130 Crk-associated substrate)-DOCK180 (Dedicator of cytokinesis 180)-RhoA (Ras homolog gene family, member A)-vinculin signaling protein complex at the apical ES, which is also an integrated component of the beta1-integrin-based adhesion protein complex based on co-immunoprecipitation experiment. It was also shown that besides p-FAK-Tyr(397) and p-FAK-Tyr(576), beta1-integrin, p130Cas, RhoA and vinculin displayed stage-specific expression in the seminiferous epithelium during the epithelial cycle with predominant localization at the apical ES as demonstrated by immunohistochemistry. Based on these findings, functional studies can now be performed to assess the role of this beta1-integrin-p-FAK-p130Cas-DOCK180-RhoA-vinculin protein complex in apical ES dynamics during spermiogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/139946
ISSN
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSiu, MKYen_US
dc.contributor.authorWong, CHen_US
dc.contributor.authorXia, Wen_US
dc.contributor.authorMruk, DDen_US
dc.contributor.authorLee, WWMen_US
dc.contributor.authorCheng, CYen_US
dc.date.accessioned2011-09-23T06:02:32Z-
dc.date.available2011-09-23T06:02:32Z-
dc.date.issued2011en_US
dc.identifier.citationSpermatogenesis, 2011, v. 1 n. 1, p. 73-86en_US
dc.identifier.issn2156-5554-
dc.identifier.urihttp://hdl.handle.net/10722/139946-
dc.description.abstractDuring spermatogenesis, step 1 spermatids (round spermatids) derive from spermatocytes following meiosis I and II at stage XIV of the epithelial cycle begin a series of morphological transformation and differentiation via 19 steps in rats to form spermatozoa. This process is known as spermiogenesis, which is marked by condensation of the genetic material in the spermatid head, formation of the acrosome and elongation of the tail. Since developing spermatids are lacking the robust protein synthesis and transcriptional activity, the cellular, molecular and morphological changes associated with spermiogenesis rely on the Sertoli cell in the seminiferous epithelium via desmosome and gap junction between Sertoli cells and step 1-7 spermatids. Interestingly, a unique anchoring junction type arises at the interface of step 8 spermatid and Sertoli cell known as apical ectoplasmic specialization (apical ES). Once it appears, apical ES is the only anchoring device restricted to the interface of step 8-19 spermatids and Sertoli cells to confer spermatid polarity, adhesion, signal communication and structural support, and to provide nutritional support during spermiogenesis, replacing desmosome and gap junction. While the adhesion protein complexes that constitute the apical ES are known, the signaling protein complexes that regulate apical ES dynamics, however, remain largely unknown. Herein we report the presence of a FAK (focal adhesion kinase)-p130Cas (p130 Crk-associated substrate)-DOCK180 (Dedicator of cytokinesis 180)-RhoA (Ras homolog gene family, member A)-vinculin signaling protein complex at the apical ES, which is also an integrated component of the beta1-integrin-based adhesion protein complex based on co-immunoprecipitation experiment. It was also shown that besides p-FAK-Tyr(397) and p-FAK-Tyr(576), beta1-integrin, p130Cas, RhoA and vinculin displayed stage-specific expression in the seminiferous epithelium during the epithelial cycle with predominant localization at the apical ES as demonstrated by immunohistochemistry. Based on these findings, functional studies can now be performed to assess the role of this beta1-integrin-p-FAK-p130Cas-DOCK180-RhoA-vinculin protein complex in apical ES dynamics during spermiogenesis.-
dc.languageengen_US
dc.publisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/spermatogenesis/-
dc.relation.ispartofSpermatogenesisen_US
dc.subjectTestis-
dc.subjectSpermatogenesis-
dc.subjectApical ectoplasmic specialization-
dc.subjectAdjudin-
dc.subjectSeminiferous epithelial cycle-
dc.titleThe beta-1-integrin-p-FAK-p130Cas-DOCK180-RhoA-vinculin is a novel regulatory protein complex at the apical ectoplasmic specialization in adult rat testesen_US
dc.typeArticleen_US
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hk, mkysiu@pathology.hku.hken_US
dc.identifier.emailLee, WWM: hrszlwm@hku.hken_US
dc.identifier.authoritySiu, MKY=rp00275en_US
dc.identifier.authorityLee, WWM=rp00728en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4161/spmg.1.1.15452-
dc.identifier.pmid21866278-
dc.identifier.pmcidPMC3158647-
dc.identifier.hkuros194360en_US
dc.identifier.volume1en_US
dc.identifier.issue1-
dc.identifier.spage73en_US
dc.identifier.epage86en_US
dc.publisher.placeUnited States-

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