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Article: Efficacy of Abbott RealTime High Risk HPV test in evaluation of atypical squamous cells of undetermined significance from an Asian screening population

TitleEfficacy of Abbott RealTime High Risk HPV test in evaluation of atypical squamous cells of undetermined significance from an Asian screening population
Authors
KeywordsAbbott RealTime High Risk HPV test
Cervical cancer
Cervical cytology
HPV DNA test
Hybrid Capture 2
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2011, v. 51 n. 2, p. 136-138 How to Cite?
AbstractBackground: Abbott RealTime High Risk HPV test is a new qualitative real-time PCR assay for the detection of 14 high risk HPV (HR-HPV) types and specific identification of HPV16 and HPV18. For each new HPV DNA test, it is important to validate its clinical performance using established tests as benchmarks. Hybrid Capture 2 (HC2) is the first USA FDA-approved HR-HPV DNA test. Objectives: To compare the performance of Abbott RealTime High Risk HPV test with that of Hybrid Capture 2 in detecting cytology samples with varying prognosis. Study design: 250 liquid-based cervical cytology samples diagnosed of Atypical Squamous cells of Undetermined Significance (ASC-US) collected from an Asian Screening Population were independently tested with both Abbott RealTime High Risk HPV test and HC2. Their utility in predicting disease progression was evaluated in 82 of the samples for which follow up cytology or colposcropic histology data was available. Results: Good to excellent agreement between the two tests was demonstrated (Kappa = 0.800, 95% CI: 0.726-0.874). The sensitivity, specificity, positive (PPV) and negative predictive values (NPV) of the two tests in detecting cases with underlying HSIL/CIN2+ were evaluated (Abbott: 100%, 20.83%, 14.93% and 100% respectively; HC2: 100%, 12.50%, 13.70% and 100% respectively). HPV16/18 genotyping provided by the Abbott test enhanced specific identification of cases with LSIL/CIN1+ (specificity 91.30%, PPV 84.62%) and HSIL/CIN2+ (specificity 86.11%, PPV 23.08%) at follow-up. Conclusions: The Abbott test performed similarly to HC2 and is unlikely to be affected by ethnicity. Abbott combined HPV detection and HPV 16/18 genotyping is found to provide enhanced sensitivity and specificity for triage of ASC-US. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/139935
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, OGWen_HK
dc.contributor.authorLo, CKen_HK
dc.contributor.authorSzeto, Een_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2011-09-23T06:02:00Z-
dc.date.available2011-09-23T06:02:00Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Clinical Virology, 2011, v. 51 n. 2, p. 136-138en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139935-
dc.description.abstractBackground: Abbott RealTime High Risk HPV test is a new qualitative real-time PCR assay for the detection of 14 high risk HPV (HR-HPV) types and specific identification of HPV16 and HPV18. For each new HPV DNA test, it is important to validate its clinical performance using established tests as benchmarks. Hybrid Capture 2 (HC2) is the first USA FDA-approved HR-HPV DNA test. Objectives: To compare the performance of Abbott RealTime High Risk HPV test with that of Hybrid Capture 2 in detecting cytology samples with varying prognosis. Study design: 250 liquid-based cervical cytology samples diagnosed of Atypical Squamous cells of Undetermined Significance (ASC-US) collected from an Asian Screening Population were independently tested with both Abbott RealTime High Risk HPV test and HC2. Their utility in predicting disease progression was evaluated in 82 of the samples for which follow up cytology or colposcropic histology data was available. Results: Good to excellent agreement between the two tests was demonstrated (Kappa = 0.800, 95% CI: 0.726-0.874). The sensitivity, specificity, positive (PPV) and negative predictive values (NPV) of the two tests in detecting cases with underlying HSIL/CIN2+ were evaluated (Abbott: 100%, 20.83%, 14.93% and 100% respectively; HC2: 100%, 12.50%, 13.70% and 100% respectively). HPV16/18 genotyping provided by the Abbott test enhanced specific identification of cases with LSIL/CIN1+ (specificity 91.30%, PPV 84.62%) and HSIL/CIN2+ (specificity 86.11%, PPV 23.08%) at follow-up. Conclusions: The Abbott test performed similarly to HC2 and is unlikely to be affected by ethnicity. Abbott combined HPV detection and HPV 16/18 genotyping is found to provide enhanced sensitivity and specificity for triage of ASC-US. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.subjectAbbott RealTime High Risk HPV testen_HK
dc.subjectCervical canceren_HK
dc.subjectCervical cytologyen_HK
dc.subjectHPV DNA testen_HK
dc.subjectHybrid Capture 2en_HK
dc.subject.meshCarcinoma, Squamous Cell - diagnosis-
dc.subject.meshEarly Detection of Cancer - methods-
dc.subject.meshPapillomavirus Infections - diagnosis-
dc.subject.meshPolymerase Chain Reaction - methods-
dc.subject.meshVirology - methods-
dc.titleEfficacy of Abbott RealTime High Risk HPV test in evaluation of atypical squamous cells of undetermined significance from an Asian screening populationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=51&issue=2&spage=136&epage=138&date=2011&atitle=Efficacy+of+Abbott+RealTime+High+Risk+HPV+test+in+evaluation+of+atypical+squamous+cells+of+undetermined+significance+from+an+Asian+screening+population-
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcv.2011.03.010en_HK
dc.identifier.pmid21530385-
dc.identifier.scopuseid_2-s2.0-79956336871en_HK
dc.identifier.hkuros192479en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956336871&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue2en_HK
dc.identifier.spage136en_HK
dc.identifier.epage138en_HK
dc.identifier.isiWOS:000290943600010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.citeulike9283294-

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