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Article: Non-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment

TitleNon-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment
Authors
KeywordsChronic hepatitis B
Host genetic risk factor
Salivary diagnostic
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/cca
Citation
Clinica Chimica Acta, 2011, v. 412 n. 11-12, p. 952-957 How to Cite?
AbstractBackground: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci. Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA. Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value. < 0.001). High genotype concordance between different sources of genomic DNA was obtained. Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/139925
ISSN
2015 Impact Factor: 2.799
2015 SCImago Journal Rankings: 1.040
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Special Administrative Region, China04050352
Funding Information:

The work described in this paper was partially supported by a grant from the Research Fund for the Control of Infectious Diseases of the Hong Kong Special Administrative Region, China (Project no. 04050352).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLau, KCen_HK
dc.contributor.authorLam, CWen_HK
dc.contributor.authorLaw, CYen_HK
dc.contributor.authorLai, STen_HK
dc.contributor.authorTsang, TYen_HK
dc.contributor.authorSiu, CWKen_HK
dc.contributor.authorTo, WKen_HK
dc.contributor.authorLeung, KFen_HK
dc.contributor.authorMak, CMen_HK
dc.contributor.authorPoon, WTen_HK
dc.contributor.authorChan, PKSen_HK
dc.contributor.authorChan, YWen_HK
dc.date.accessioned2011-09-23T06:01:32Z-
dc.date.available2011-09-23T06:01:32Z-
dc.date.issued2011en_HK
dc.identifier.citationClinica Chimica Acta, 2011, v. 412 n. 11-12, p. 952-957en_HK
dc.identifier.issn0009-8981en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139925-
dc.description.abstractBackground: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci. Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA. Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value. < 0.001). High genotype concordance between different sources of genomic DNA was obtained. Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ccaen_HK
dc.relation.ispartofClinica Chimica Actaen_HK
dc.subjectChronic hepatitis Ben_HK
dc.subjectHost genetic risk factoren_HK
dc.subjectSalivary diagnosticen_HK
dc.titleNon-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-8981&volume=412&issue=11-12&spage=952&epage=957&date=2011&atitle=Non-invasive+screening+of+HLA-DPA1+and+HLA-DPB1+alleles+for+persistent+hepatitis+B+virus+infection:+susceptibility+for+vertical+transmission+and+toward+a+personalized+approach+for+vaccination+and+treatment-
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_HK
dc.identifier.emailLaw, CY:ericlaw@pathology.hku.hken_HK
dc.identifier.authorityLam, CW=rp00260en_HK
dc.identifier.authorityLaw, CY=rp01586en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cca.2011.01.030en_HK
dc.identifier.pmid21310144-
dc.identifier.scopuseid_2-s2.0-79955046277en_HK
dc.identifier.hkuros192457en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955046277&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume412en_HK
dc.identifier.issue11-12en_HK
dc.identifier.spage952en_HK
dc.identifier.epage957en_HK
dc.identifier.eissn1873-3492-
dc.identifier.isiWOS:000291125200025-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectWhole genome scan of genetic susceptibility for hepatitis B carries-
dc.identifier.citeulike8790625-

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