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Article: Non-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment
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TitleNon-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment
 
AuthorsLau, KC1
Lam, CW1
Law, CY2
Lai, ST2
Tsang, TY2
Siu, CWK2
To, WK
Leung, KF1
Mak, CM2
Poon, WT2
Chan, PKS3
Chan, YW2
 
KeywordsChronic hepatitis B
Host genetic risk factor
Salivary diagnostic
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/cca
 
CitationClinica Chimica Acta, 2011, v. 412 n. 11-12, p. 952-957 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cca.2011.01.030
 
AbstractBackground: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci. Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA. Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value. < 0.001). High genotype concordance between different sources of genomic DNA was obtained. Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. © 2011 Elsevier B.V.
 
ISSN0009-8981
2013 Impact Factor: 2.764
2013 SCImago Journal Rankings: 1.039
 
DOIhttp://dx.doi.org/10.1016/j.cca.2011.01.030
 
ISI Accession Number IDWOS:000291125200025
Funding AgencyGrant Number
Hong Kong Special Administrative Region, China04050352
Funding Information:

The work described in this paper was partially supported by a grant from the Research Fund for the Control of Infectious Diseases of the Hong Kong Special Administrative Region, China (Project no. 04050352).

 
ReferencesReferences in Scopus
 
GrantsWhole genome scan of genetic susceptibility for hepatitis B carries
 
DC FieldValue
dc.contributor.authorLau, KC
 
dc.contributor.authorLam, CW
 
dc.contributor.authorLaw, CY
 
dc.contributor.authorLai, ST
 
dc.contributor.authorTsang, TY
 
dc.contributor.authorSiu, CWK
 
dc.contributor.authorTo, WK
 
dc.contributor.authorLeung, KF
 
dc.contributor.authorMak, CM
 
dc.contributor.authorPoon, WT
 
dc.contributor.authorChan, PKS
 
dc.contributor.authorChan, YW
 
dc.date.accessioned2011-09-23T06:01:32Z
 
dc.date.available2011-09-23T06:01:32Z
 
dc.date.issued2011
 
dc.description.abstractBackground: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci. Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA. Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value. < 0.001). High genotype concordance between different sources of genomic DNA was obtained. Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationClinica Chimica Acta, 2011, v. 412 n. 11-12, p. 952-957 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cca.2011.01.030
 
dc.identifier.citeulike8790625
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.cca.2011.01.030
 
dc.identifier.eissn1873-3492
 
dc.identifier.epage957
 
dc.identifier.hkuros192457
 
dc.identifier.isiWOS:000291125200025
Funding AgencyGrant Number
Hong Kong Special Administrative Region, China04050352
Funding Information:

The work described in this paper was partially supported by a grant from the Research Fund for the Control of Infectious Diseases of the Hong Kong Special Administrative Region, China (Project no. 04050352).

 
dc.identifier.issn0009-8981
2013 Impact Factor: 2.764
2013 SCImago Journal Rankings: 1.039
 
dc.identifier.issue11-12
 
dc.identifier.openurl
 
dc.identifier.pmid21310144
 
dc.identifier.scopuseid_2-s2.0-79955046277
 
dc.identifier.spage952
 
dc.identifier.urihttp://hdl.handle.net/10722/139925
 
dc.identifier.volume412
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/cca
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofClinica Chimica Acta
 
dc.relation.projectWhole genome scan of genetic susceptibility for hepatitis B carries
 
dc.relation.referencesReferences in Scopus
 
dc.subjectChronic hepatitis B
 
dc.subjectHost genetic risk factor
 
dc.subjectSalivary diagnostic
 
dc.titleNon-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Princess Margaret Hospital Hong Kong
  3. Chinese University of Hong Kong