File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube

TitleExpression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube
Authors
KeywordsChlamydia trachomatis
Decidua
Ectopic pregnancy
Endometrium
Smoking
Issue Date2010
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2010, v. 16 n. 12, p. 950-959 How to Cite?
AbstractWe investigated whether the repulsive SLIT/ROBO pathway is expressed in the endometrium and is negatively regulated during implantation. We also examined whether deficient expression in the Fallopian tube (FT) may predispose to ectopic pregnancy (EP). Endometrium (n = 21) and FT (n = 17) were collected across the menstrual cycle from fertile women with regular cycles. Decidualized endometrium (n = 6) was obtained from women undergoing termination, and FT (n = 6) was obtained from women with EP. SLIT/ROBO expression was quantified by reverse transcription-PCR and protein localized by immunohistochemistry. The regulation of SLIT/ROBO expression in vitro, by sex steroids and hCG, was assessed in endometrial (hTERT-EEpC) epithelial cells, and the effects of Chlamydia trachomatis infection and smoking were studied in oviductal (OE-E6/E7) epithelial cells. Endometrial SLIT3 was highest in the mid-secretory phase (P = 0.0003) and SLIT1,2 and ROBO1 showed a similar trend. ROBO2 was highest in proliferative phase (P = 0.027) and ROBO3,4 showed a similar trend. SLIT2,3 and ROBO1, 4 were lower in decidua compared with mid-secretory endometrium (P <0.05). SLITs and ROBOs, excepting ROBO2, were expressed in FT but there were no differences across the cycle or in EP. SLIT/ROBO proteins were localized to endometrial and FT epithelium. Treatment of hTERT-EEpC with a combination of estradiol and medroxyprogesterone acetate inhibited ROBO1 expression (P <0.01) but hCG had no effect. Acute treatment of OE-E6/E7 with smoking metabolite, cotinine, and C. trachomatis had no effect. These findings imply a regulated role for the endometrial SLIT/ROBO interaction during normal development and pregnancy but that it may not be important in the aetiology of EP. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/139907
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
SFC
Chief Scientists OfficeSCD/02
Wellbeing of WomenR40608
MRC
Funding Information:

This work was supported by a SFC Senior Clinical Research Fellowship to W. C. D. with funding from the Chief Scientists Office (SCD/02) and by a grant from Wellbeing of Women (R40608) to A. W. H. A. W. H. is supported by an MRC Clinician Scientist Fellowship.

References

 

DC FieldValueLanguage
dc.contributor.authorDuncan, WCen_HK
dc.contributor.authorMcDonald, SEen_HK
dc.contributor.authorDickinson, REen_HK
dc.contributor.authorShaw, JLVen_HK
dc.contributor.authorLourenco, PCen_HK
dc.contributor.authorWheelhouse, Nen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorCritchley, HODen_HK
dc.contributor.authorHorne, AWen_HK
dc.date.accessioned2011-09-23T06:00:03Z-
dc.date.available2011-09-23T06:00:03Z-
dc.date.issued2010en_HK
dc.identifier.citationMolecular Human Reproduction, 2010, v. 16 n. 12, p. 950-959en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139907-
dc.description.abstractWe investigated whether the repulsive SLIT/ROBO pathway is expressed in the endometrium and is negatively regulated during implantation. We also examined whether deficient expression in the Fallopian tube (FT) may predispose to ectopic pregnancy (EP). Endometrium (n = 21) and FT (n = 17) were collected across the menstrual cycle from fertile women with regular cycles. Decidualized endometrium (n = 6) was obtained from women undergoing termination, and FT (n = 6) was obtained from women with EP. SLIT/ROBO expression was quantified by reverse transcription-PCR and protein localized by immunohistochemistry. The regulation of SLIT/ROBO expression in vitro, by sex steroids and hCG, was assessed in endometrial (hTERT-EEpC) epithelial cells, and the effects of Chlamydia trachomatis infection and smoking were studied in oviductal (OE-E6/E7) epithelial cells. Endometrial SLIT3 was highest in the mid-secretory phase (P = 0.0003) and SLIT1,2 and ROBO1 showed a similar trend. ROBO2 was highest in proliferative phase (P = 0.027) and ROBO3,4 showed a similar trend. SLIT2,3 and ROBO1, 4 were lower in decidua compared with mid-secretory endometrium (P <0.05). SLITs and ROBOs, excepting ROBO2, were expressed in FT but there were no differences across the cycle or in EP. SLIT/ROBO proteins were localized to endometrial and FT epithelium. Treatment of hTERT-EEpC with a combination of estradiol and medroxyprogesterone acetate inhibited ROBO1 expression (P <0.01) but hCG had no effect. Acute treatment of OE-E6/E7 with smoking metabolite, cotinine, and C. trachomatis had no effect. These findings imply a regulated role for the endometrial SLIT/ROBO interaction during normal development and pregnancy but that it may not be important in the aetiology of EP. © The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.subjectChlamydia trachomatisen_HK
dc.subjectDeciduaen_HK
dc.subjectEctopic pregnancyen_HK
dc.subjectEndometriumen_HK
dc.subjectSmokingen_HK
dc.subject.meshEndometrium - metabolism-
dc.subject.meshFallopian Tubes - metabolism-
dc.subject.meshNerve Tissue Proteins - analysis - genetics - metabolism-
dc.subject.meshReceptors, Immunologic - analysis - genetics - metabolism-
dc.subject.meshSignal Transduction - drug effects - genetics-
dc.titleExpression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tubeen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/molehr/gaq055en_HK
dc.identifier.pmid20651036en_HK
dc.identifier.pmcidPMC2992050-
dc.identifier.scopuseid_2-s2.0-78649474322en_HK
dc.identifier.hkuros196028en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649474322&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue12en_HK
dc.identifier.spage950en_HK
dc.identifier.epage959en_HK
dc.identifier.isiWOS:000284639000009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDuncan, WC=7201615551en_HK
dc.identifier.scopusauthoridMcDonald, SE=12780378500en_HK
dc.identifier.scopusauthoridDickinson, RE=25221025500en_HK
dc.identifier.scopusauthoridShaw, JLV=7403899035en_HK
dc.identifier.scopusauthoridLourenco, PC=7004615649en_HK
dc.identifier.scopusauthoridWheelhouse, N=16044431200en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridCritchley, HOD=7006731536en_HK
dc.identifier.scopusauthoridHorne, AW=8067885000en_HK
dc.identifier.citeulike8403173-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats