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Article: Zona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influx

TitleZona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influx
Authors
Chiu, PCNWong, BSTLee, CLLam, KKWChung, MKLee, KFKoistinen, RKoistinen, HGupta, SKSeppälä, MYeung, WSB
KeywordsERK
glycodelin
intracellular calcium
sperm acrosome reaction
zona pellucida
Issue Date2010
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2010, v. 25 n. 11, p. 2721-2733 How to Cite?
DOI: http://dx.doi.org/10.1093/humrep/deq243
AbstractBackground Glycodelin-A interacts with spermatozoa before fertilization, but its role in modulating sperm functions is not known. Zona pellucida-induced acrosome reaction is crucial to fertilization and its dysfunction is a cause of male infertility. We hypothesized that glycodelin-A, a glycoprotein found in the female reproductive tract, potentiates human spermatozoa for zona pellucida-induced acrosome reaction. Methods Glycodelin isoforms were immunoaffinity purified. The sperm intracellular cAMP concentration, protein kinase-A (PKA) and extracellular signal-regulated kinase (ERK) activities, and intracellular calcium were measured by ELISA, kinase activity assay kits and Fluo-4AM technique, respectively. The phosphorylation of inositol 1,4,5-trisphosphate type-1 receptor (IP3R1) mediated by ERK was determined by western blotting. Zona pellucida-induced acrosome reaction was detected by Pisum sativum staining. Results Pretreatment of spermatozoa with glycodelin-A significantly up-regulated adenylyl cyclase/PKA activity and down-regulated the activity of ERK and its phosphorylation of IP3R1, thereby enhancing zona pellucida-induced calcium influx and zona pellucida-induced acrosome reaction. Glycodelin-F or deglycosylated glycodelin-A did not have these actions. Treatment of spermatozoa with a protein kinase inhibitor abolished the priming activity of glycodelin-A, whilst ERK pathway inhibitors mimic the stimulatory effect of glycodelin-A on zona pellucida-induced acrosome reaction. Conclusions Glycodelin-A in the female reproductive tract sensitizes spermatozoa for zona pellucida-induced acrosome reaction in a glycosylation-specific manner through activation of the adenylyl cyclase/PKA pathway, suppression of extracellular signal-regulated kinase activation and up-regulation of zona pellucida-induced calcium influx. © 2010 The Author.
Persistent Identifierhttp://hdl.handle.net/10722/139900
ISSN
0268-1161
2021 Impact Factor: 6.353
2020 SCImago Journal Rankings: 2.446
ISI Accession Number ID
WOS:000283124000005
Funding AgencyGrant Number
Research Grant Council, Hong KongHKU 7514/05M
HKU 7647/06M
Centre of Reproduction, Development and Growth, Academy of Finland
Helsinki University Central Hospital
Funding Information:

This work was supported by grants from the Research Grant Council, Hong Kong (HKU 7514/05M and HKU 7647/06M), Centre of Reproduction, Development and Growth, the Academy of Finland and the Helsinki University Central Hospital Research Fund.

References
References in Scopus
Grants
Role of olfactomedin in implantation
The mechanism of action of a novel glycodelin isoform from cumulus matrix on stimulating spermatozoa-zona pellucida binding

 

DC FieldValueLanguage
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorWong, BSTen_HK
dc.contributor.authorLee, CLen_HK
dc.contributor.authorLam, KKWen_HK
dc.contributor.authorChung, MKen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorKoistinen, Ren_HK
dc.contributor.authorKoistinen, Hen_HK
dc.contributor.authorGupta, SKen_HK
dc.contributor.authorSeppälä, Men_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2011-09-23T05:59:50Z-
dc.date.available2011-09-23T05:59:50Z-
dc.date.issued2010en_HK
dc.identifier.citationHuman Reproduction, 2010, v. 25 n. 11, p. 2721-2733en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139900-
dc.description.abstractBackground Glycodelin-A interacts with spermatozoa before fertilization, but its role in modulating sperm functions is not known. Zona pellucida-induced acrosome reaction is crucial to fertilization and its dysfunction is a cause of male infertility. We hypothesized that glycodelin-A, a glycoprotein found in the female reproductive tract, potentiates human spermatozoa for zona pellucida-induced acrosome reaction. Methods Glycodelin isoforms were immunoaffinity purified. The sperm intracellular cAMP concentration, protein kinase-A (PKA) and extracellular signal-regulated kinase (ERK) activities, and intracellular calcium were measured by ELISA, kinase activity assay kits and Fluo-4AM technique, respectively. The phosphorylation of inositol 1,4,5-trisphosphate type-1 receptor (IP3R1) mediated by ERK was determined by western blotting. Zona pellucida-induced acrosome reaction was detected by Pisum sativum staining. Results Pretreatment of spermatozoa with glycodelin-A significantly up-regulated adenylyl cyclase/PKA activity and down-regulated the activity of ERK and its phosphorylation of IP3R1, thereby enhancing zona pellucida-induced calcium influx and zona pellucida-induced acrosome reaction. Glycodelin-F or deglycosylated glycodelin-A did not have these actions. Treatment of spermatozoa with a protein kinase inhibitor abolished the priming activity of glycodelin-A, whilst ERK pathway inhibitors mimic the stimulatory effect of glycodelin-A on zona pellucida-induced acrosome reaction. Conclusions Glycodelin-A in the female reproductive tract sensitizes spermatozoa for zona pellucida-induced acrosome reaction in a glycosylation-specific manner through activation of the adenylyl cyclase/PKA pathway, suppression of extracellular signal-regulated kinase activation and up-regulation of zona pellucida-induced calcium influx. © 2010 The Author.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 2010, v. 25 n. 11, p. 2721-2733 is available online at: http://humrep.oxfordjournals.org/content/25/11/2721-
dc.subjectERKen_HK
dc.subjectglycodelinen_HK
dc.subjectintracellular calciumen_HK
dc.subjectsperm acrosome reactionen_HK
dc.subjectzona pellucidaen_HK
dc.subject.meshAcrosome Reaction - drug effects - physiology-
dc.subject.meshAdenylate Cyclase - metabolism-
dc.subject.meshCalcium - metabolism-
dc.subject.meshExtracellular Signal-Regulated MAP Kinases - metabolism-
dc.subject.meshGlycoproteins - physiology-
dc.titleZona pellucida-induced acrosome reaction in human spermatozoa is potentiated by glycodelin-A via down-regulation of extracellular signal-regulated kinases and up-regulation of zona pellucida-induced calcium influxen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=25&issue=11&spage=2721&epage=2733&date=2010&atitle=Zona+pellucida-induced+acrosome+reaction+in+human+spermatozoa+is+potentiated+by+glycodelin-A+via+down-regulation+of+extracellular+signal-regulated+kinases+and+up-regulation+of+zona+pellucida-induced+calcium+influx-
dc.identifier.emailChiu, PCN:pchiucn@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1093/humrep/deq243en_HK
dc.identifier.pmid20843776-
dc.identifier.scopuseid_2-s2.0-77958140629en_HK
dc.identifier.hkuros195594en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77958140629&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2721en_HK
dc.identifier.epage2733en_HK
dc.identifier.isiWOS:000283124000005-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectRole of olfactomedin in implantation-
dc.relation.projectThe mechanism of action of a novel glycodelin isoform from cumulus matrix on stimulating spermatozoa-zona pellucida binding-
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridWong, BST=24337009700en_HK
dc.identifier.scopusauthoridLee, CL=9277221100en_HK
dc.identifier.scopusauthoridLam, KKW=25637362300en_HK
dc.identifier.scopusauthoridChung, MK=8964203600en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridKoistinen, R=7006574669en_HK
dc.identifier.scopusauthoridKoistinen, H=7003612125en_HK
dc.identifier.scopusauthoridGupta, SK=23978174000en_HK
dc.identifier.scopusauthoridSeppälä, M=35475165300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.citeulike7864113-
dc.identifier.issnl0268-1161-

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