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Article: Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells

TitleMultiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells
Authors
Issue Date2011
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261
Citation
Tissue Engineering Part C: Methods, 2011, v. 17 n. 5, p. 579-588 How to Cite?
AbstractNano- and microscale topographical cues play critical roles in the induction and maintenance of various cellular functions, including morphology, adhesion, gene regulation, and communication. Recent studies indicate that structure and function at the heart tissue level is exquisitely sensitive to mechanical cues at the nano-scale as well as at the microscale level. Although fabrication methods exist for generating topographical features for cell culture, current techniques, especially those with nanoscale resolution, are typically complex, prohibitively expensive, and not accessible to most biology laboratories. Here, we present a tunable culture platform comprised of biomimetic wrinkles that simulate the heart's complex anisotropic and multiscale architecture for facile and robust cardiac cell alignment. We demonstrate the cellular and subcellular alignment of both neonatal mouse cardiomyocytes as well as those derived from human embryonic stem cells. By mimicking the fibrillar network of the extracellular matrix, this system enables monitoring of protein localization in real time and therefore the high-resolution study of phenotypic and physiologic responses to in-vivo like topographical cues.
Persistent Identifierhttp://hdl.handle.net/10722/139804
ISSN
2015 SCImago Journal Rankings: 1.141
ISI Accession Number ID
Funding AgencyGrant Number
California Institute of Regenerative Medicine (CIRM)
Shrink Nanotechnologies
Funding Information:

Funding for this project was supported by the California Institute of Regenerative Medicine (CIRM) and Shrink Nanotechnologies. We would also like to thank UC Irvine's INRF facilities and Chi-cheng Fu.

References

 

DC FieldValueLanguage
dc.contributor.authorLuna, JIen_HK
dc.contributor.authorCiriza, Jen_HK
dc.contributor.authorGarcia-Ojeda, MEen_HK
dc.contributor.authorKong, Men_HK
dc.contributor.authorHerren, Aen_HK
dc.contributor.authorLieu, DKen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorFowlkes, CCen_HK
dc.contributor.authorKhine, Men_HK
dc.contributor.authorMcCloskey, KEen_HK
dc.date.accessioned2011-09-23T05:56:24Z-
dc.date.available2011-09-23T05:56:24Z-
dc.date.issued2011en_HK
dc.identifier.citationTissue Engineering Part C: Methods, 2011, v. 17 n. 5, p. 579-588en_HK
dc.identifier.issn1937-3384en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139804-
dc.description.abstractNano- and microscale topographical cues play critical roles in the induction and maintenance of various cellular functions, including morphology, adhesion, gene regulation, and communication. Recent studies indicate that structure and function at the heart tissue level is exquisitely sensitive to mechanical cues at the nano-scale as well as at the microscale level. Although fabrication methods exist for generating topographical features for cell culture, current techniques, especially those with nanoscale resolution, are typically complex, prohibitively expensive, and not accessible to most biology laboratories. Here, we present a tunable culture platform comprised of biomimetic wrinkles that simulate the heart's complex anisotropic and multiscale architecture for facile and robust cardiac cell alignment. We demonstrate the cellular and subcellular alignment of both neonatal mouse cardiomyocytes as well as those derived from human embryonic stem cells. By mimicking the fibrillar network of the extracellular matrix, this system enables monitoring of protein localization in real time and therefore the high-resolution study of phenotypic and physiologic responses to in-vivo like topographical cues.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261en_HK
dc.relation.ispartofTissue Engineering Part C: Methodsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Tissue Engineering Part C: Methods] © [2011] [copyright Mary Ann Liebert, Inc.]; [Tissue Engineering Part C: Methods]-
dc.subject.meshBiomimetics - methods-
dc.subject.meshEmbryonic Stem Cells - cytology - metabolism-
dc.subject.meshExtracellular Matrix - metabolism-
dc.subject.meshMyocytes, Cardiac - cytology - metabolism-
dc.subject.meshTissue Engineering - methods-
dc.titleMultiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-3384&volume=17&issue=5&spage=579&epage=588&date=2011&atitle=Multiscale+biomimetic+topography+for+the+alignment+of+neonatal+and+embryonic+stem+cell-derived+heart+cells-
dc.identifier.emailKong, M: marcokong@hku.hken_HK
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hken_HK
dc.identifier.authorityKong, M=rp01563en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/ten.TEC.2010.0410en_HK
dc.identifier.pmid21235325-
dc.identifier.scopuseid_2-s2.0-79955376296en_HK
dc.identifier.hkuros192193en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955376296&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue5en_HK
dc.identifier.spage579en_HK
dc.identifier.epage588en_HK
dc.identifier.isiWOS:000289937100008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMcCloskey, KE=7006661312en_HK
dc.identifier.scopusauthoridKhine, M=23110958700en_HK
dc.identifier.scopusauthoridFowlkes, CC=6701549596en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridLieu, DK=7003924538en_HK
dc.identifier.scopusauthoridHerren, A=37004474100en_HK
dc.identifier.scopusauthoridKong, M=36784634200en_HK
dc.identifier.scopusauthoridGarciaOjeda, ME=6507550795en_HK
dc.identifier.scopusauthoridCiriza, J=8344919300en_HK
dc.identifier.scopusauthoridLuna, JI=35201725400en_HK

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