File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: M 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veins

TitleM 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veins
Authors
Issue Date2011
PublisherOceanSide Publications, Inc. The Journal's web site is located at http://www.oceansidepubl.com/ajr/index.htm
Citation
American Journal Of Rhinology And Allergy, 2011, v. 25 n. 2, p. e60-e65 How to Cite?
AbstractBackground: Acetylcholine (ACh) has been shown to induce nasal congestion via vasorelaxation of intranasal posterior collecting veins (PCV) coupled with vasocontraction of extranasal outflow veins (dorsal nasal vein [DNV] and sphenopalatine vein [SPV]). The aim of this study was to characterize the muscarinic receptor subtype(s) involved in ACh-induced relaxation and contraction in canine nasal veins. Methods: PCV, DNV, and SPV were isolated from the canine nose. In vitro isometric tension of segments from these veins was monitored to reflect vascular reactivity. ACh concentration-response curve was studied in the presence of muscarinic receptor subtype inhibitors. Immunohistochemical localization of M 1-M 5 receptor subtypes in the veins was performed. Results: ACh-induced relaxation in PVC was inhibited by pertussis toxin (PTX; inhibitor of G-protein that couples M2/M4 receptors), methoctramine (selective M 2 muscarinic receptor inhibitor), muscarinic toxin 7 (MT-7; selective M 1 muscarinic receptor inhibitor), and 4-diphenylacetoxy-methylpiperidine methiodide (4-DAMP; selective M3 muscarinic receptor inhibitor). ACh-induced contraction in SPV and DNV was potentiated by PTX and methoctramine but was inhibited by MT-7 and 4-DAMP. Immunohistochemistry confirmed the presence of five muscarinic receptor subtypes in the endothelium of nasal veins, with staining of M 3 > M 1 > M 5 > M 2 > M 4 in PVC but M 2 > M 4 > M 3 > M 1 > M 5 in outflow veins. M1 and M3 receptor subtypes were localized in the smooth muscles of both types of veins. Conclusion: The results show that ACh relaxes intranasal veins and contracts extranasal veins primarily via M 1 and M 3 muscarinic receptor subtypes, implying the therapeutic value of M 1/M 3-specific or highly selective anticholinergics on nasal congestion. Copyright © 2011, OceanSide Publications, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/139663
ISSN
2015 Impact Factor: 1.96
2015 SCImago Journal Rankings: 1.055
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200607176113
Funding Information:

Supported by CRCG Grant 200607176113 from The University of Hong Kong

References

 

DC FieldValueLanguage
dc.contributor.authorLung, MAen_HK
dc.date.accessioned2011-09-23T05:53:10Z-
dc.date.available2011-09-23T05:53:10Z-
dc.date.issued2011en_HK
dc.identifier.citationAmerican Journal Of Rhinology And Allergy, 2011, v. 25 n. 2, p. e60-e65en_HK
dc.identifier.issn1945-8924en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139663-
dc.description.abstractBackground: Acetylcholine (ACh) has been shown to induce nasal congestion via vasorelaxation of intranasal posterior collecting veins (PCV) coupled with vasocontraction of extranasal outflow veins (dorsal nasal vein [DNV] and sphenopalatine vein [SPV]). The aim of this study was to characterize the muscarinic receptor subtype(s) involved in ACh-induced relaxation and contraction in canine nasal veins. Methods: PCV, DNV, and SPV were isolated from the canine nose. In vitro isometric tension of segments from these veins was monitored to reflect vascular reactivity. ACh concentration-response curve was studied in the presence of muscarinic receptor subtype inhibitors. Immunohistochemical localization of M 1-M 5 receptor subtypes in the veins was performed. Results: ACh-induced relaxation in PVC was inhibited by pertussis toxin (PTX; inhibitor of G-protein that couples M2/M4 receptors), methoctramine (selective M 2 muscarinic receptor inhibitor), muscarinic toxin 7 (MT-7; selective M 1 muscarinic receptor inhibitor), and 4-diphenylacetoxy-methylpiperidine methiodide (4-DAMP; selective M3 muscarinic receptor inhibitor). ACh-induced contraction in SPV and DNV was potentiated by PTX and methoctramine but was inhibited by MT-7 and 4-DAMP. Immunohistochemistry confirmed the presence of five muscarinic receptor subtypes in the endothelium of nasal veins, with staining of M 3 > M 1 > M 5 > M 2 > M 4 in PVC but M 2 > M 4 > M 3 > M 1 > M 5 in outflow veins. M1 and M3 receptor subtypes were localized in the smooth muscles of both types of veins. Conclusion: The results show that ACh relaxes intranasal veins and contracts extranasal veins primarily via M 1 and M 3 muscarinic receptor subtypes, implying the therapeutic value of M 1/M 3-specific or highly selective anticholinergics on nasal congestion. Copyright © 2011, OceanSide Publications, Inc.en_HK
dc.languageengen_US
dc.publisherOceanSide Publications, Inc. The Journal's web site is located at http://www.oceansidepubl.com/ajr/index.htmen_HK
dc.relation.ispartofAmerican Journal of Rhinology and Allergyen_HK
dc.subject.meshAcetylcholine - pharmacology-
dc.subject.meshNasal Obstruction - drug therapy-
dc.subject.meshReceptor, Muscarinic M1 - antagonists and inhibitors - metabolism-
dc.subject.meshReceptor, Muscarinic M3 - antagonists and inhibitors - metabolism-
dc.subject.meshVeins - drug effects - metabolism - pathology-
dc.titleM 1and M 3 muscarinic receptors mediate relaxation and contraction in canine nasal veinsen_HK
dc.typeArticleen_HK
dc.identifier.emailLung, MA: makylung@hkucc.hku.hken_HK
dc.identifier.authorityLung, MA=rp00319en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2500/ajra.2011.25.3604en_HK
dc.identifier.pmid21679501-
dc.identifier.scopuseid_2-s2.0-79953248653en_HK
dc.identifier.hkuros194223en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953248653&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue2en_HK
dc.identifier.spagee60en_HK
dc.identifier.epagee65en_HK
dc.identifier.eissn1945-8932-
dc.identifier.isiWOS:000292636400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLung, MA=7006411781en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats