Article: Successive influenza virus infection and Streptococcus pneumoniae stimulation alter human dendritic cell function
| Title | Successive influenza virus infection and Streptococcus pneumoniae stimulation alter human dendritic cell function | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Wu, Y1 Mao, H1 Ling, MT1 Chow, KH1 Ho, PL1 Tu, W1 Lau, YL1 | ||||||||||
| Issue Date | 2011 | ||||||||||
| Publisher | BioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcinfectdis/ | ||||||||||
| Citation | Bmc Infectious Diseases, 2011, v. 11 [How to Cite?] DOI: http://dx.doi.org/10.1186/1471-2334-11-201 | ||||||||||
| Abstract | Background: Influenza virus is a major cause of respiratory disease worldwide and Streptococcus pneumoniae infection associated with influenza often leads to severe complications. Dendritic cells are key antigen presenting cells but its role in such co-infection is unclear.Methods: In this study, human monocyte derived-dentritic cells were either concurrently or successively challenged with the combination of live influenza virus and heat killed pneumococcus to mimic the viral pneumococcal infection. Dendritic cell viability, phenotypic maturation and cytokine production were then examined.Results: The challenge of influenza virus and pneumococcus altered dendritic cell functions dependent on the time interval between the successive challenge of influenza virus and pneumococcus, as well as the doses of pneumococcus. When dendritic cells were exposed to pneumococcus at 6 hr, but not 0 hr nor 24 hr after influenza virus infection, both virus and pneumococcus treated dendritic cells had greater cell apoptosis and expressed higher CD83 and CD86 than dendritic cells infected with influenza virus alone. Dendritic cells produced pro-inflammatory cytokines: TNF-α, IL-12 and IFN-γ synergistically to the successive viral and pneumococcal challenge. Whereas prior influenza virus infection suppressed the IL-10 response independent of the timing of the subsequent pneumococcal stimulation.Conclusions: Our results demonstrated that successive challenge of dendritic cells with influenza virus and pneumococcus resulted in synergistic up-regulation of pro-inflammatory cytokines with simultaneous down-regulation of anti-inflammatory cytokine, which may explain the immuno-pathogenesis of this important co-infection. © 2011 Wu et al; licensee BioMed Central Ltd. | ||||||||||
| ISSN | 1471-2334 2011 Impact Factor: 3.118 2011 SCImago Journal Rankings: 0.263 | ||||||||||
| DOI | http://dx.doi.org/10.1186/1471-2334-11-201 | ||||||||||
| ISI Accession Number ID | WOS:000293283200001
Funding Information: This work was supported in part by the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (Project No AoE/M-12/06); General Research Fund, Research Grants Council of Hong Kong, (HKU 777108M, HKU777407, HKU768108); a Commission Grant from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government (2009-2014, Lab-11); Edward Sai-Kim Hotung Paediatric Education and Research Fund. Technical support of the use of flow cytometry from Faculty Core Facility. | ||||||||||
| PubMed Central ID | PMC3146832 | ||||||||||
| References | References in Scopus | ||||||||||
| Grants | Control of Pandemic and Inter-pandemic Influenza The Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection |
| dc.contributor.author | Wu, Y | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Mao, H | ||||||||||
| dc.contributor.author | Ling, MT | ||||||||||
| dc.contributor.author | Chow, KH | ||||||||||
| dc.contributor.author | Ho, PL | ||||||||||
| dc.contributor.author | Tu, W | ||||||||||
| dc.contributor.author | Lau, YL | ||||||||||
| dc.date.accessioned | 2011-09-23T05:51:54Z | ||||||||||
| dc.date.available | 2011-09-23T05:51:54Z | ||||||||||
| dc.date.issued | 2011 | ||||||||||
| dc.description.abstract | Background: Influenza virus is a major cause of respiratory disease worldwide and Streptococcus pneumoniae infection associated with influenza often leads to severe complications. Dendritic cells are key antigen presenting cells but its role in such co-infection is unclear.Methods: In this study, human monocyte derived-dentritic cells were either concurrently or successively challenged with the combination of live influenza virus and heat killed pneumococcus to mimic the viral pneumococcal infection. Dendritic cell viability, phenotypic maturation and cytokine production were then examined.Results: The challenge of influenza virus and pneumococcus altered dendritic cell functions dependent on the time interval between the successive challenge of influenza virus and pneumococcus, as well as the doses of pneumococcus. When dendritic cells were exposed to pneumococcus at 6 hr, but not 0 hr nor 24 hr after influenza virus infection, both virus and pneumococcus treated dendritic cells had greater cell apoptosis and expressed higher CD83 and CD86 than dendritic cells infected with influenza virus alone. Dendritic cells produced pro-inflammatory cytokines: TNF-α, IL-12 and IFN-γ synergistically to the successive viral and pneumococcal challenge. Whereas prior influenza virus infection suppressed the IL-10 response independent of the timing of the subsequent pneumococcal stimulation.Conclusions: Our results demonstrated that successive challenge of dendritic cells with influenza virus and pneumococcus resulted in synergistic up-regulation of pro-inflammatory cytokines with simultaneous down-regulation of anti-inflammatory cytokine, which may explain the immuno-pathogenesis of this important co-infection. © 2011 Wu et al; licensee BioMed Central Ltd. | ||||||||||
| dc.description.grant | Control of Pandemic and Inter-pandemic Influenza | ||||||||||
| dc.description.grant | The Role of Natural Killer Cells in the Pathogenesis of Avian Influenza Virus Infection | ||||||||||
| dc.description.grantcode | 97655 | ||||||||||
| dc.description.grantcode | 98640 | ||||||||||
| dc.description.nature | published_or_final_version | ||||||||||
| dc.identifier.citation | Bmc Infectious Diseases, 2011, v. 11 [How to Cite?] DOI: http://dx.doi.org/10.1186/1471-2334-11-201 | ||||||||||
| dc.identifier.citeulike | 9573224 | ||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1186/1471-2334-11-201 | ||||||||||
| dc.identifier.hkuros | 192241 | ||||||||||
| dc.identifier.hkuros | 192241 | ||||||||||
| dc.identifier.hkuros | 200730 | ||||||||||
| dc.identifier.isi | WOS:000293283200001
Funding Information: This work was supported in part by the Area of Excellence program on Influenza supported by the University Grants Committee of the Hong Kong SAR, China (Project No AoE/M-12/06); General Research Fund, Research Grants Council of Hong Kong, (HKU 777108M, HKU777407, HKU768108); a Commission Grant from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health, Welfare and Food Bureau of the Hong Kong SAR Government (2009-2014, Lab-11); Edward Sai-Kim Hotung Paediatric Education and Research Fund. Technical support of the use of flow cytometry from Faculty Core Facility. | ||||||||||
| dc.identifier.issn | 1471-2334 2011 Impact Factor: 3.118 2011 SCImago Journal Rankings: 0.263 | ||||||||||
| dc.identifier.pmcid | PMC3146832 | ||||||||||
| dc.identifier.pmid | 21771345 | ||||||||||
| dc.identifier.scopus | eid_2-s2.0-79960556668 | ||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/139573 | ||||||||||
| dc.identifier.volume | 11 | ||||||||||
| dc.language | eng | ||||||||||
| dc.publisher | BioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcinfectdis/ | ||||||||||
| dc.publisher.place | United Kingdom | ||||||||||
| dc.relation.ispartof | BMC Infectious Diseases | ||||||||||
| dc.relation.references | References in Scopus | ||||||||||
| dc.rights | BMC Infectious Diseases. Copyright © BioMed Central. | ||||||||||
| dc.subject.mesh | Dendritic Cells - immunology - microbiology - virology | ||||||||||
| dc.subject.mesh | Influenza A Virus, H1N1 Subtype - immunology | ||||||||||
| dc.subject.mesh | Influenza, Human - immunology | ||||||||||
| dc.subject.mesh | Pneumococcal Infections - immunology | ||||||||||
| dc.subject.mesh | Streptococcus pneumoniae - immunology | ||||||||||
| dc.title | Successive influenza virus infection and Streptococcus pneumoniae stimulation alter human dendritic cell function | ||||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine