Article: Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA
| Title | Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Hu, X1 3 Li, P3 Yeung, KWK1 Chu, PK2 Wu, S1 2 3 Xu, Z1 2 3 | ||||||
| Keywords | Cationic DNA Fluorescent Gene delivery carrier Rare earth | ||||||
| Issue Date | 2011 | ||||||
| Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htm | ||||||
| Citation | Colloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00396-011-2472-2 | ||||||
| Abstract | A complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag. | ||||||
| ISSN | 0303-402X 2011 Impact Factor: 2.331 2011 SCImago Journal Rankings: 0.126 | ||||||
| DOI | http://dx.doi.org/10.1007/s00396-011-2472-2 | ||||||
| ISI Accession Number ID | WOS:000292975800005
Funding Information: This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002). The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively. | ||||||
| References | References in Scopus |
| dc.contributor.author | Hu, X | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Li, P | ||||||
| dc.contributor.author | Yeung, KWK | ||||||
| dc.contributor.author | Chu, PK | ||||||
| dc.contributor.author | Wu, S | ||||||
| dc.contributor.author | Xu, Z | ||||||
| dc.date.accessioned | 2011-09-23T05:51:37Z | ||||||
| dc.date.available | 2011-09-23T05:51:37Z | ||||||
| dc.date.issued | 2011 | ||||||
| dc.description.abstract | A complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Colloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00396-011-2472-2 | ||||||
| dc.identifier.citeulike | 9514032 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s00396-011-2472-2 | ||||||
| dc.identifier.epage | 1468 | ||||||
| dc.identifier.hkuros | 192190 | ||||||
| dc.identifier.isi | WOS:000292975800005
Funding Information: This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002). The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively. | ||||||
| dc.identifier.issn | 0303-402X 2011 Impact Factor: 2.331 2011 SCImago Journal Rankings: 0.126 | ||||||
| dc.identifier.issue | 13 | ||||||
| dc.identifier.scopus | eid_2-s2.0-80052469651 | ||||||
| dc.identifier.spage | 1459 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/139556 | ||||||
| dc.identifier.volume | 289 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htm | ||||||
| dc.publisher.place | Germany | ||||||
| dc.relation.ispartof | Colloid and Polymer Science | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject | Cationic | ||||||
| dc.subject | DNA | ||||||
| dc.subject | Fluorescent | ||||||
| dc.subject | Gene delivery carrier | ||||||
| dc.subject | Rare earth | ||||||
| dc.title | Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- City University of Hong Kong
- Hubei University