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Article: Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA

TitlePreparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA
Authors
KeywordsCationic
DNA
Fluorescent
Gene delivery carrier
Rare earth
Issue Date2011
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htm
Citation
Colloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468 How to Cite?
AbstractA complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/139556
ISSN
2014 Impact Factor: 1.865
2014 SCImago Journal Rankings: 0.607
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China50973027
Specialized Research Fund for the Doctoral Program of Higher Education20094208110002
Funding Information:

This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002). The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively.

References

 

DC FieldValueLanguage
dc.contributor.authorHu, Xen_HK
dc.contributor.authorLi, Pen_HK
dc.contributor.authorYeung, KWKen_HK
dc.contributor.authorChu, PKen_HK
dc.contributor.authorWu, Sen_HK
dc.contributor.authorXu, Zen_HK
dc.date.accessioned2011-09-23T05:51:37Z-
dc.date.available2011-09-23T05:51:37Z-
dc.date.issued2011en_HK
dc.identifier.citationColloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468en_HK
dc.identifier.issn0303-402Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/139556-
dc.description.abstractA complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htmen_HK
dc.relation.ispartofColloid and Polymer Scienceen_HK
dc.subjectCationicen_HK
dc.subjectDNAen_HK
dc.subjectFluorescenten_HK
dc.subjectGene delivery carrieren_HK
dc.subjectRare earthen_HK
dc.titlePreparation, characterization of cationic terbium luminescent copolymer and its interaction with DNAen_HK
dc.typeArticleen_HK
dc.identifier.emailYeung, KWK:wkkyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, KWK=rp00309en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00396-011-2472-2en_HK
dc.identifier.scopuseid_2-s2.0-80052469651en_HK
dc.identifier.hkuros192190en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052469651&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume289en_HK
dc.identifier.issue13en_HK
dc.identifier.spage1459en_HK
dc.identifier.epage1468en_HK
dc.identifier.isiWOS:000292975800005-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHu, X=8650009700en_HK
dc.identifier.scopusauthoridLi, P=7404773164en_HK
dc.identifier.scopusauthoridYeung, KWK=13309584700en_HK
dc.identifier.scopusauthoridChu, PK=36040705700en_HK
dc.identifier.scopusauthoridWu, S=15125218800en_HK
dc.identifier.scopusauthoridXu, Z=12760201200en_HK
dc.identifier.citeulike9514032-

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