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Article: Preparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA
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TitlePreparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA
 
AuthorsHu, X1 3
Li, P3
Yeung, KWK1
Chu, PK2
Wu, S3 1 2
Xu, Z3 1 2
 
KeywordsCationic
DNA
Fluorescent
Gene delivery carrier
Rare earth
 
Issue Date2011
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htm
 
CitationColloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00396-011-2472-2
 
AbstractA complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag.
 
ISSN0303-402X
2012 Impact Factor: 2.161
2012 SCImago Journal Rankings: 0.778
 
DOIhttp://dx.doi.org/10.1007/s00396-011-2472-2
 
ISI Accession Number IDWOS:000292975800005
Funding AgencyGrant Number
National Natural Science Foundation of China50973027
Specialized Research Fund for the Doctoral Program of Higher Education20094208110002
Funding Information:

This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002). The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHu, X
 
dc.contributor.authorLi, P
 
dc.contributor.authorYeung, KWK
 
dc.contributor.authorChu, PK
 
dc.contributor.authorWu, S
 
dc.contributor.authorXu, Z
 
dc.date.accessioned2011-09-23T05:51:37Z
 
dc.date.available2011-09-23T05:51:37Z
 
dc.date.issued2011
 
dc.description.abstractA complex of Tb 3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Tb(AA) 3Phen•H 2O was characterized with elemental analysis, FT-IR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly (METAC-co-NIPAm-co-Tb(AA) 3Phen•H 2O) (PMNTb), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile (AIBN) as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PMNTb with DNA was studied by fluorescence spectroscopy, UV-vis spectroscopy, and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PMNTb could interact with DNA in an electrostatic bonding mode and the bonding constant was 3.4×10 5 L mol -1. The TEM observation showed that the PMNTb could form micelles in water solution; the efficient complexation of PMNTb with DNA occurred. The results of in vitro cytotoxicity indicated that PMNTb had good biocompability. These results laid the foundation for the further study in potential gene detective reagent and gene delivery carrier. © 2011 Springer-Verlag.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationColloid And Polymer Science, 2011, v. 289 n. 13, p. 1459-1468 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00396-011-2472-2
 
dc.identifier.citeulike9514032
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00396-011-2472-2
 
dc.identifier.epage1468
 
dc.identifier.hkuros192190
 
dc.identifier.isiWOS:000292975800005
Funding AgencyGrant Number
National Natural Science Foundation of China50973027
Specialized Research Fund for the Doctoral Program of Higher Education20094208110002
Funding Information:

This work is supported by the National Natural Science Foundation of China (No. 50973027) and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20094208110002). The authors are grateful to Prof. Gongwu Song and his group, Faculty of Chemistry and Chemical Engineering, for their help in fluorescence spectra determination; Prof. Lixin Ma and his group, Faculty of Biology Science, for their help in DNA complexation determination and in vitro cytotoxicity, respectively.

 
dc.identifier.issn0303-402X
2012 Impact Factor: 2.161
2012 SCImago Journal Rankings: 0.778
 
dc.identifier.issue13
 
dc.identifier.scopuseid_2-s2.0-80052469651
 
dc.identifier.spage1459
 
dc.identifier.urihttp://hdl.handle.net/10722/139556
 
dc.identifier.volume289
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00396/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofColloid and Polymer Science
 
dc.relation.referencesReferences in Scopus
 
dc.subjectCationic
 
dc.subjectDNA
 
dc.subjectFluorescent
 
dc.subjectGene delivery carrier
 
dc.subjectRare earth
 
dc.titlePreparation, characterization of cationic terbium luminescent copolymer and its interaction with DNA
 
dc.typeArticle
 
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<contributor.author>Wu, S</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
  3. Hubei University