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Article: Transport genes and chemotaxis in Laribacter hongkongensis: a genome-wide analysis

TitleTransport genes and chemotaxis in Laribacter hongkongensis: a genome-wide analysis
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cellandbioscience.com
Citation
Cell & Bioscience, 2011, v. 1, article no. 28 How to Cite?
AbstractABSTRACT: BACKGROUND: Laribacter hongkongensis is a Gram-negative, sea gull-shaped rod associated with community-acquired gastroenteritis. The bacterium has been found in diverse freshwater environments including fish, frogs and drinking water reservoirs. Using the complete genome sequence data of L. hongkongensis, we performed a comprehensive analysis of putative transport-related genes and genes related to chemotaxis, motility and quorum sensing, which may help the bacterium adapt to the changing environments and combat harmful substances. RESULTS: A genome-wide analysis using Transport Classification Database TCDB, similarity and keyword searches revealed the presence of a large diversity of transporters (n = 457) and genes related to chemotaxis (n = 52) and flagellar biosynthesis (n = 40) in the L. hongkongensis genome. The transporters included those from all seven major transporter categories, which may allow the uptake of essential nutrients or ions, and extrusion of metabolic end products and hazardous substances. L. hongkongensis is unique among closely related members of Neisseriaceae family in possessing higher number of proteins related to transport of ammonium, urea and dicarboxylate, which may reflect the importance of nitrogen and dicarboxylate metabolism in this assacharolytic bacterium. Structural modeling of two C4-dicarboxylate transporters showed that they possessed similar structures to the determined structures of other DctP-TRAP transporters, with one having an unusual disulfide bond. Diverse mechanisms for iron transport, including hemin transporters for iron acquisition from host proteins, were also identified. In addition to the chemotaxis and flagella-related genes, the L. hongkongensis genome also contained two copies of qseB/qseC homologues of the AI-3 quorum sensing system. CONCLUSIONS: The large number of diverse transporters and genes involved in chemotaxis, motility and quorum sensing suggested that the bacterium may utilize a complex system to adapt to different environments. Structural modeling will provide useful insights on the transporters in L. hongkongensis.
Persistent Identifierhttp://hdl.handle.net/10722/139520
ISSN
2015 Impact Factor: 2.883
2015 SCImago Journal Rankings: 1.593
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_US
dc.contributor.authorFan, RYYen_US
dc.contributor.authorWong, GKMen_US
dc.contributor.authorTeng, JLLen_US
dc.contributor.authorSze, KHen_US
dc.contributor.authorTse, Hen_US
dc.contributor.authorYuen, KYen_US
dc.contributor.authorWoo, PCY-
dc.date.accessioned2011-09-23T05:51:03Z-
dc.date.available2011-09-23T05:51:03Z-
dc.date.issued2011en_US
dc.identifier.citationCell & Bioscience, 2011, v. 1, article no. 28en_US
dc.identifier.issn2045-3701-
dc.identifier.urihttp://hdl.handle.net/10722/139520-
dc.description.abstractABSTRACT: BACKGROUND: Laribacter hongkongensis is a Gram-negative, sea gull-shaped rod associated with community-acquired gastroenteritis. The bacterium has been found in diverse freshwater environments including fish, frogs and drinking water reservoirs. Using the complete genome sequence data of L. hongkongensis, we performed a comprehensive analysis of putative transport-related genes and genes related to chemotaxis, motility and quorum sensing, which may help the bacterium adapt to the changing environments and combat harmful substances. RESULTS: A genome-wide analysis using Transport Classification Database TCDB, similarity and keyword searches revealed the presence of a large diversity of transporters (n = 457) and genes related to chemotaxis (n = 52) and flagellar biosynthesis (n = 40) in the L. hongkongensis genome. The transporters included those from all seven major transporter categories, which may allow the uptake of essential nutrients or ions, and extrusion of metabolic end products and hazardous substances. L. hongkongensis is unique among closely related members of Neisseriaceae family in possessing higher number of proteins related to transport of ammonium, urea and dicarboxylate, which may reflect the importance of nitrogen and dicarboxylate metabolism in this assacharolytic bacterium. Structural modeling of two C4-dicarboxylate transporters showed that they possessed similar structures to the determined structures of other DctP-TRAP transporters, with one having an unusual disulfide bond. Diverse mechanisms for iron transport, including hemin transporters for iron acquisition from host proteins, were also identified. In addition to the chemotaxis and flagella-related genes, the L. hongkongensis genome also contained two copies of qseB/qseC homologues of the AI-3 quorum sensing system. CONCLUSIONS: The large number of diverse transporters and genes involved in chemotaxis, motility and quorum sensing suggested that the bacterium may utilize a complex system to adapt to different environments. Structural modeling will provide useful insights on the transporters in L. hongkongensis.-
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cellandbioscience.com-
dc.relation.ispartofCell & Bioscienceen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleTransport genes and chemotaxis in Laribacter hongkongensis: a genome-wide analysisen_US
dc.typeArticleen_US
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_US
dc.identifier.emailFan, RYY: rfyy@hku.hken_US
dc.identifier.emailTeng, JLL: llteng@hku.hken_US
dc.identifier.emailSze, KH: khsze@hku.hken_US
dc.identifier.emailTse, H: herman@graduate.hku.hken_US
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_US
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486en_US
dc.identifier.authorityTeng, JLL=rp00277en_US
dc.identifier.authoritySze, KH=rp00785en_US
dc.identifier.authorityTse, H=rp00519en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/2045-3701-1-28-
dc.identifier.pmid21849034-
dc.identifier.pmcidPMC3180692-
dc.identifier.scopuseid_2-s2.0-84862896545-
dc.identifier.hkuros195240en_US
dc.identifier.volume1, article no. 28en_US
dc.identifier.isiWOS:000307057500001-
dc.publisher.placeUnited Kingdom-
dc.identifier.citeulike10716283-

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