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Article: Rediscovery and genomic characterization of bovine astroviruses

TitleRediscovery and genomic characterization of bovine astroviruses
Authors
Issue Date2011
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2011, v. 92 n. 8, p. 1888-1898 How to Cite?
AbstractThe genus Mamastrovirus belongs to the family Astroviridae and consists of at least six members infecting different mammalian hosts, including humans, cattle and pigs. In recent years, novel astroviruses have been identified in other mammalian species like roe deer, bats and sea lions. While the bovine astrovirus was one of the earliest astroviruses to have been studied, no further research has been performed recently and its genome sequence remains uncharacterized. In this report, we describe the detection and genomic characterization of astroviruses in bovine faecal specimens obtained in Hong Kong. Five of 209 specimens were found to be positive for astrovirus by RT-PCR. Two of the positive specimens were found to contain sequences from two different astrovirus strains. Complete genome sequences of approximately 6.3 kb in length were obtained for four strains, which showed similar organization of the genome compared to other astroviruses. Phylogenetic analysis confirmed their identities as members of the genus Mamastrovirus, and showed them to be most closely related to the Capreolus capreolus astrovirus. Based on the pairwise genetic distances among their full-length ORF2 sequences, these bovine astroviruses may be assigned into at least three different genotype species. Sequence analysis revealed evidence of potential recombination in ORF2. In summary, we report the first genome sequences of bovine astroviruses and clearly establish the species status of the virus. Additionally, our study is among the first to report co-infection by different astrovirus genotypes in the same host, which is an essential step for recombination to occur. © 2011 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/139518
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council
University Development Fund
Outstanding Young Researcher Award
University of Hong Kong
Tung Wah Group of Hospitals
Health, Welfare and Food Bureau
Shaw Foundation
Funding Information:

We thank Professor York Y. N. Chow, Secretary of Health, Welfare and Food, HKSAR; Dr Constance Chan, Controller for the Centre for Food Safety, FEHD, HKSAR; and other staff of the FEHD, HKSAR. Views expressed in this paper are those of the authors only, and do not represent the opinion of the FEHD or the Government of the HKSAR. We are grateful for the generous support of Mr Hui Hoy and Mr Hui Ming in the genomic sequencing platform. This work was partly supported by the Research Grant Council, University Development Fund and Outstanding Young Researcher Award, The University of Hong Kong; the Tung Wah Group of Hospitals Fund for Research in Infectious Diseases; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; and the Shaw Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorTse, Hen_HK
dc.contributor.authorChan, WMen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorFan, RYYen_HK
dc.contributor.authorLau, CCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2011-09-23T05:51:01Z-
dc.date.available2011-09-23T05:51:01Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of General Virology, 2011, v. 92 n. 8, p. 1888-1898en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139518-
dc.description.abstractThe genus Mamastrovirus belongs to the family Astroviridae and consists of at least six members infecting different mammalian hosts, including humans, cattle and pigs. In recent years, novel astroviruses have been identified in other mammalian species like roe deer, bats and sea lions. While the bovine astrovirus was one of the earliest astroviruses to have been studied, no further research has been performed recently and its genome sequence remains uncharacterized. In this report, we describe the detection and genomic characterization of astroviruses in bovine faecal specimens obtained in Hong Kong. Five of 209 specimens were found to be positive for astrovirus by RT-PCR. Two of the positive specimens were found to contain sequences from two different astrovirus strains. Complete genome sequences of approximately 6.3 kb in length were obtained for four strains, which showed similar organization of the genome compared to other astroviruses. Phylogenetic analysis confirmed their identities as members of the genus Mamastrovirus, and showed them to be most closely related to the Capreolus capreolus astrovirus. Based on the pairwise genetic distances among their full-length ORF2 sequences, these bovine astroviruses may be assigned into at least three different genotype species. Sequence analysis revealed evidence of potential recombination in ORF2. In summary, we report the first genome sequences of bovine astroviruses and clearly establish the species status of the virus. Additionally, our study is among the first to report co-infection by different astrovirus genotypes in the same host, which is an essential step for recombination to occur. © 2011 SGM.en_HK
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.subject.meshAstroviridae - classification - genetics - isolation and purification-
dc.subject.meshAstroviridae Infections - veterinary - virology-
dc.subject.meshCattle-
dc.subject.meshCattle Diseases - virology-
dc.subject.meshGenomics-
dc.titleRediscovery and genomic characterization of bovine astrovirusesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=92&issue=8&spage=1888&epage=1898&date=2011&atitle=Rediscovery+and+genomic+characterization+of+bovine+astroviruses-
dc.identifier.emailTse, H:herman@graduate.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/vir.0.030817-0en_HK
dc.identifier.pmid21508185-
dc.identifier.scopuseid_2-s2.0-79960350217en_HK
dc.identifier.hkuros195237en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960350217&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1888en_HK
dc.identifier.epage1898en_HK
dc.identifier.isiWOS:000293488800018-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridChan, WM=7403918969en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridFan, RYY=15519269300en_HK
dc.identifier.scopusauthoridLau, CCY=8398162900en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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