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Article: Transgenic Plasmodium that expresses HIV-1 Gag elicits immunity and protects mice against vaccinia virus-gag and malarial parasites

TitleTransgenic Plasmodium that expresses HIV-1 Gag elicits immunity and protects mice against vaccinia virus-gag and malarial parasites
Authors
KeywordsHIV-1
Transgenic Plasmodium berghei
Vaccine
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
Citation
Vaccine, 2010, v. 28 n. 50, p. 7915-7922 How to Cite?
AbstractMalaria and human immunodeficiency virus type 1 (HIV-1) infection overlap in many regions of the world. Our goal was to determine the feasibility of developing transgenic Plasmodium berghei that expresses HIV-1 Gag, PbGAG, as a conceptual bivalent vaccine against both HIV-1 infection and malaria. Immunization of mice with PbGAG induced specific responses to the HIV-1 Gag. Importantly, mice vaccinated with PbGAG were significantly protected from challenge with vaccinia virus-gag (VV-gag) with an average 30-fold reduction in titer (P<. 0.05). In addition, mice immunized with PbGAG developed Plasmodium-specific immune responses and the immunized animals were protected from challenges with blood-stage P. berghei NK65 and Plasmodium yoelii 17XL. We demonstrated a novel vaccination strategy that uses a live transgenic protozoan parasite-based bivalent vaccine to immunize mice and confer significant levels of protection against VV-gag and malarial parasite challenges. These observations have important implications for the development of a new form of bivalent vaccine against both HIV-1 and malaria. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/139513
ISSN
2015 Impact Factor: 3.413
2015 SCImago Journal Rankings: 2.044
ISI Accession Number ID
Funding AgencyGrant Number
Chinese Academy of SciencesKSCX1-YW-10
National Basic Research Program of China2006CB504208
Funding Information:

Our special thanks are given to Prof. Zhong Su for critical review of this manuscript and to Junqiang Xu and Min Guan for providing technical assistance. We thank NIH AIDS Research and Reference Reagent Program for providing HIV-1 Gag peptides (Catalog Number 8117), and thank MR4 for providing malaria parasite P. berghei NK65 contributed by V. Nussenzweig and for providing plasmid pL0015 contributed by A. Waters. We thank Professor Yaming Cao for providing malaria parasite P. yoelii 17XL. This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX1-YW-10) and the National Basic Research Program of China (2006CB504208).

References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Ben_HK
dc.contributor.authorQin, Len_HK
dc.contributor.authorDu, Yen_HK
dc.contributor.authorPeng, Nen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorChen, Zen_HK
dc.contributor.authorChen, Xen_HK
dc.date.accessioned2011-09-23T05:50:57Z-
dc.date.available2011-09-23T05:50:57Z-
dc.date.issued2010en_HK
dc.identifier.citationVaccine, 2010, v. 28 n. 50, p. 7915-7922en_HK
dc.identifier.issn0264-410Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/139513-
dc.description.abstractMalaria and human immunodeficiency virus type 1 (HIV-1) infection overlap in many regions of the world. Our goal was to determine the feasibility of developing transgenic Plasmodium berghei that expresses HIV-1 Gag, PbGAG, as a conceptual bivalent vaccine against both HIV-1 infection and malaria. Immunization of mice with PbGAG induced specific responses to the HIV-1 Gag. Importantly, mice vaccinated with PbGAG were significantly protected from challenge with vaccinia virus-gag (VV-gag) with an average 30-fold reduction in titer (P<. 0.05). In addition, mice immunized with PbGAG developed Plasmodium-specific immune responses and the immunized animals were protected from challenges with blood-stage P. berghei NK65 and Plasmodium yoelii 17XL. We demonstrated a novel vaccination strategy that uses a live transgenic protozoan parasite-based bivalent vaccine to immunize mice and confer significant levels of protection against VV-gag and malarial parasite challenges. These observations have important implications for the development of a new form of bivalent vaccine against both HIV-1 and malaria. © 2010 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccineen_HK
dc.relation.ispartofVaccineen_HK
dc.subjectHIV-1en_HK
dc.subjectTransgenic Plasmodium bergheien_HK
dc.subjectVaccineen_HK
dc.subject.meshMalaria - immunology - prevention and control-
dc.subject.meshMalaria Vaccines - immunology-
dc.subject.meshPlasmodium berghei - genetics - immunology-
dc.subject.meshVaccinia - immunology - prevention and control-
dc.subject.meshgag Gene Products, Human Immunodeficiency Virus - immunology-
dc.titleTransgenic Plasmodium that expresses HIV-1 Gag elicits immunity and protects mice against vaccinia virus-gag and malarial parasitesen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, Z:zchenai@hkucc.hku.hken_HK
dc.identifier.authorityChen, Z=rp00243en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vaccine.2010.09.075en_HK
dc.identifier.pmid20933565-
dc.identifier.scopuseid_2-s2.0-78650255639en_HK
dc.identifier.hkuros192317en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650255639&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue50en_HK
dc.identifier.spage7915en_HK
dc.identifier.epage7922en_HK
dc.identifier.isiWOS:000285667400012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridJiang, B=37034190700en_HK
dc.identifier.scopusauthoridQin, L=35811121500en_HK
dc.identifier.scopusauthoridDu, Y=37033670700en_HK
dc.identifier.scopusauthoridPeng, N=36679213800en_HK
dc.identifier.scopusauthoridChen, L=35723672300en_HK
dc.identifier.scopusauthoridChen, Z=35271180800en_HK
dc.identifier.scopusauthoridChen, X=35483484300en_HK
dc.identifier.citeulike8011960-

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