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Article: Complete genome analysis of three novel picornaviruses from diverse bat species

TitleComplete genome analysis of three novel picornaviruses from diverse bat species
Authors
Issue Date2011
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2011, v. 85 n. 17, p. 8819-8828 How to Cite?
AbstractAlthough bats are important reservoirs of diverse viruses that can cause human epidemics, little is known about the presence of picornaviruses in these flying mammals. Among 1,108 bats of 18 species studied, three novel picornaviruses (groups 1, 2, and 3) were identified from alimentary specimens of 12 bats from five species and four genera. Two complete genomes, each from the three picornaviruses, were sequenced. Phylogenetic analysis showed that they fell into three distinct clusters in the Picornaviridae family, with low homologies to known picornaviruses, especially in leader and 2A proteins. Moreover, group 1 and 2 viruses are more closely related to each other than to group 3 viruses, which exhibit genome features distinct from those of the former two virus groups. In particular, the group 3 virus genome contains the shortest leader protein within Picornaviridae, a putative type I internal ribosome entry site (IRES) in the 5′-untranslated region instead of the type IV IRES found in group 1 and 2 viruses, one instead of two GXCG motifs in 2A, an L→V substitution in the DDLXQ motif in 2C helicase, and a conserved GXH motif in 3C protease. Group 1 and 2 viruses are unique among picornaviruses in having AMH instead of the GXH motif in 3C pro. These findings suggest that the three picornaviruses belong to two novel genera in the Picornaviridae family. This report describes the discovery and complete genome analysis of three picornaviruses in bats, and their presence in diverse bat genera/species suggests the ability to cross the species barrier. © 2011, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/139512
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
HKSAR Department of Agriculture, Fisheries, and Conservation [AFCD]
Hong Kong Police Force
Research Grant Council Grant
University Grant Council
Strategic Research Theme Fund
Committee for Research and Conference
University Development Fund, The University of Hong Kong
Tung Wah Group of Hospitals
Welfare and Food Bureau
Providence Foundation Limited
Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health
Funding Information:

We thank Alan Chi-Kong Wong, Siu-Fai Leung, Chik-Chuen Lay, Ping-Man So, and K. F. Chan (HKSAR Department of Agriculture, Fisheries, and Conservation [AFCD]) and the Hong Kong Police Force for facilitation and support; Cynthia S. M. Chan and Joseph W. K. So from AFCD; and King-Shun Lo (Laboratory Animal Unit) and Cassius Chan for their excellent technical assistance and collection of animal specimens. We are grateful for the generous support of Carol Yu, Richard Yu, Hui Hoy, and Hui Ming in the genomic sequencing platform.

References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLai, KKYen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorYip, CCYen_HK
dc.contributor.authorShek, CTen_HK
dc.contributor.authorLee, Pen_HK
dc.contributor.authorLam, CSFen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2011-09-23T05:50:57Z-
dc.date.available2011-09-23T05:50:57Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Virology, 2011, v. 85 n. 17, p. 8819-8828en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/139512-
dc.description.abstractAlthough bats are important reservoirs of diverse viruses that can cause human epidemics, little is known about the presence of picornaviruses in these flying mammals. Among 1,108 bats of 18 species studied, three novel picornaviruses (groups 1, 2, and 3) were identified from alimentary specimens of 12 bats from five species and four genera. Two complete genomes, each from the three picornaviruses, were sequenced. Phylogenetic analysis showed that they fell into three distinct clusters in the Picornaviridae family, with low homologies to known picornaviruses, especially in leader and 2A proteins. Moreover, group 1 and 2 viruses are more closely related to each other than to group 3 viruses, which exhibit genome features distinct from those of the former two virus groups. In particular, the group 3 virus genome contains the shortest leader protein within Picornaviridae, a putative type I internal ribosome entry site (IRES) in the 5′-untranslated region instead of the type IV IRES found in group 1 and 2 viruses, one instead of two GXCG motifs in 2A, an L→V substitution in the DDLXQ motif in 2C helicase, and a conserved GXH motif in 3C protease. Group 1 and 2 viruses are unique among picornaviruses in having AMH instead of the GXH motif in 3C pro. These findings suggest that the three picornaviruses belong to two novel genera in the Picornaviridae family. This report describes the discovery and complete genome analysis of three picornaviruses in bats, and their presence in diverse bat genera/species suggests the ability to cross the species barrier. © 2011, American Society for Microbiology.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.subject.meshChiroptera - virology-
dc.subject.meshGenome, Viral-
dc.subject.meshPicornaviridae - classification - genetics - isolation and purification-
dc.subject.meshRNA, Viral - genetics-
dc.subject.meshSequence Analysis, DNA-
dc.titleComplete genome analysis of three novel picornaviruses from diverse bat speciesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=85&issue=17&spage=8819&epage=8828&date=2011&atitle=Complete+genome+analysis+of+three+novel+picornaviruses+from+diverse+bat+species-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYip, CCY: yipcyril@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYip, CCY=rp01721en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JVI.02364-10en_HK
dc.identifier.pmid21697464-
dc.identifier.pmcidPMC3165794-
dc.identifier.scopuseid_2-s2.0-80052275129en_HK
dc.identifier.hkuros192207en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052275129&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume85en_HK
dc.identifier.issue17en_HK
dc.identifier.spage8819en_HK
dc.identifier.epage8828en_HK
dc.identifier.isiWOS:000293626100034-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLai, KKY=36708103200en_HK
dc.identifier.scopusauthoridHuang, Y=35597414700en_HK
dc.identifier.scopusauthoridYip, CCY=14016999800en_HK
dc.identifier.scopusauthoridShek, CT=36007758300en_HK
dc.identifier.scopusauthoridLee, P=36189338700en_HK
dc.identifier.scopusauthoridLam, CSF=25950267400en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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