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Article: Co-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in members of the family Parvoviridae, and evidence for inter-and intra-host genetic diversity and recombination

TitleCo-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in members of the family Parvoviridae, and evidence for inter-and intra-host genetic diversity and recombination
Authors
Issue Date2011
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2011, v. 92 n. 9, p. 2047-2059 How to Cite?
AbstractDespite the recent discovery of novel bocaviruses from porcine samples, their genetic evolution and diversity are poorly understood. This study reports the identification and complete genome characterization of two novel parvoviruses, porcine bocavirus 3 (PBoV3) and porcine bocavirus 4 (PBoV4), from various porcine tissues/samples, displaying marked intra-and inter-host genetic diversity, with recombination events. Bocaviruses were detected by PCR among 16.5% (55/333) of porcine samples (lymph nodes, serum, nasopharyngeal and faecal samples) from healthy, sick or deceased pigs from farms and a slaughterhouse in Hong Kong. As marked nucleotide polymorphisms were observed in the partial VP1 sequences, complete VP1 genes from one nasopharyngeal and three faecal specimens were cloned and sequenced, which suggested the presence of two different bocaviruses and demonstrated significant intra-and inter-host genetic diversity. Complete genome sequences revealed the presence of two bocaviruses, PBoV3 and PBoV4, in a faecal and nasopharyngeal specimen, respectively, with two genotypes, PBoV4-1 and PBoV4-2, in the latter. Their genomes encoded three ORFs, characteristic of bocaviruses. Phylogenetic analysis showed that they were distantly related to other bocaviruses, forming a distinct cluster within the genus. Recombination analysis showed possible recombination events among VP1 sequences of PBoV4 strains from a faecal specimen, with two breakpoints identified (with a 68 and 71 bp region), suggesting that different strains/variants within the same host could have arisen from recombination. This is the first report describing marked sequence diversity and the co-existence of two viruses of the family Parvoviridae within the same host, which may have originated from and, in turn, facilitated recombination. © 2011 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/139511
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council
University Grant Council
Strategic Research Theme Fund, Committee for Research and Conference Grant
University Development Fund
University of Hong Kong
HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau
Providence Foundation Limited
Funding Information:

We would like to thank Professor York Y. N. Chow, Secretary of Health, Welfare and Food, HKSAR, PR China; Mr Clement Leung, Dr Constance Chan and Dr W. K. Au of the Food and Environmental Hygiene Department; Mr Alan Wong and Dr Thomas Sit of the Agriculture, Fisheries and Conservation Department; Dr Thomas H. F. Tsang, Center for Health Protection, Department of Health; and the Government of the HKSAR, for their facilitation and support. We are grateful to the generous support of Mrs Carol Yu, Professor Richard Yu, Mr Hui Hoy and Mr Hui Ming in the genomic sequencing platform. This work was partly supported by the Research Grant Council Grant, University Grant Council, Strategic Research Theme Fund, Committee for Research and Conference Grant and University Development Fund, The University of Hong Kong; the HKSAR Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureau; the Providence Foundation Limited in memory of the late Dr Lui Hac Minh; and Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the HKSAR Department of Health.

References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYip, CCYen_HK
dc.contributor.authorLi, KSMen_HK
dc.contributor.authorFu, CTYen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2011-09-23T05:50:55Z-
dc.date.available2011-09-23T05:50:55Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of General Virology, 2011, v. 92 n. 9, p. 2047-2059en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139511-
dc.description.abstractDespite the recent discovery of novel bocaviruses from porcine samples, their genetic evolution and diversity are poorly understood. This study reports the identification and complete genome characterization of two novel parvoviruses, porcine bocavirus 3 (PBoV3) and porcine bocavirus 4 (PBoV4), from various porcine tissues/samples, displaying marked intra-and inter-host genetic diversity, with recombination events. Bocaviruses were detected by PCR among 16.5% (55/333) of porcine samples (lymph nodes, serum, nasopharyngeal and faecal samples) from healthy, sick or deceased pigs from farms and a slaughterhouse in Hong Kong. As marked nucleotide polymorphisms were observed in the partial VP1 sequences, complete VP1 genes from one nasopharyngeal and three faecal specimens were cloned and sequenced, which suggested the presence of two different bocaviruses and demonstrated significant intra-and inter-host genetic diversity. Complete genome sequences revealed the presence of two bocaviruses, PBoV3 and PBoV4, in a faecal and nasopharyngeal specimen, respectively, with two genotypes, PBoV4-1 and PBoV4-2, in the latter. Their genomes encoded three ORFs, characteristic of bocaviruses. Phylogenetic analysis showed that they were distantly related to other bocaviruses, forming a distinct cluster within the genus. Recombination analysis showed possible recombination events among VP1 sequences of PBoV4 strains from a faecal specimen, with two breakpoints identified (with a 68 and 71 bp region), suggesting that different strains/variants within the same host could have arisen from recombination. This is the first report describing marked sequence diversity and the co-existence of two viruses of the family Parvoviridae within the same host, which may have originated from and, in turn, facilitated recombination. © 2011 SGM.en_HK
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.subject.meshBocavirus - classification - genetics - isolation and purification-
dc.subject.meshGenetic Variation-
dc.subject.meshParvoviridae Infections - veterinary - virology-
dc.subject.meshRecombination, Genetic-
dc.subject.meshSwine Diseases - virology-
dc.titleCo-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in members of the family Parvoviridae, and evidence for inter-and intra-host genetic diversity and recombinationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=92&issue=9&spage=2047&epage=2059&date=2011&atitle=Co-existence+of+multiple+strains+of+two+novel+porcine+bocaviruses+in+the+same+pig,+a+previously+undescribed+phenomenon+in+members+of+the+family+Parvoviridae+and+evidence+for+inter-+and+intra-host+genetic+diversity+and+recombination-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYip, CCY: yipcyril@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYip, CCY=rp01721en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/vir.0.033688-0en_HK
dc.identifier.pmid21632566-
dc.identifier.scopuseid_2-s2.0-80051740535en_HK
dc.identifier.hkuros192205en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80051740535&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2047en_HK
dc.identifier.epage2059en_HK
dc.identifier.isiWOS:000295018900007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYip, CCY=14016999800en_HK
dc.identifier.scopusauthoridLi, KSM=24759122500en_HK
dc.identifier.scopusauthoridFu, CTY=24740766100en_HK
dc.identifier.scopusauthoridHuang, Y=35597414700en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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