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Article: In vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheres

TitleIn vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheres
Authors
KeywordsCalcified cartilage
Collagen microspheres
Complex tissue engineering
Mesenchymal stem cells
Microencapsulation
Osteochondral interface
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2011, v. 32 n. 6, p. 1526-1535 How to Cite?
AbstractCreating biological interfaces between mechanically dissimilar tissues is a key challenge in complex tissue engineering. An osteochondral interface is essential in preventing mechanical failure and maintaining normal function of cartilage. Despite tremendous efforts in developing osteochondral plugs, formation of the osteochondral interface with proper zonal organization has not yet been reported. Here, we present a mesenchymal stem cell-collagen microsphere-based approach for complex tissue engineering and demonstrate in vitro formation of a stem cell-derived osteochondral interface with calcified cartilage interface separating a non-calcified cartilage layer and an underlying bone layer. Cells at the interface region are hypertrophic chondrocytes while the extracellular matrix in this region contains collagen type II and X, calcium deposits and vertically running fibers. The simultaneous presence of appropriate medium and configuration during co-culture is necessary for the interface formation. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/139425
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
Funding AgencyGrant Number
Innovation and Technology Commission
Hong Kong GovernmentITS-133-08
University of Hong Kong on Biomedical Engineering and Bionanotechnology
Funding Information:

This work was supported by grants from the Innovation and Technology Commission, the Hong Kong Government (ITS-133-08) and the Strategic Research Themes of the University of Hong Kong on Biomedical Engineering and Bionanotechnology. The authors thank Dr. YY Li for assistance with the animal work, Ms. ST Chow for assistance with the BMP2 measurement experiment and Ms. Vicki Geall for editing the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorCheng, HWen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2011-09-23T05:49:22Z-
dc.date.available2011-09-23T05:49:22Z-
dc.date.issued2011en_HK
dc.identifier.citationBiomaterials, 2011, v. 32 n. 6, p. 1526-1535en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139425-
dc.description.abstractCreating biological interfaces between mechanically dissimilar tissues is a key challenge in complex tissue engineering. An osteochondral interface is essential in preventing mechanical failure and maintaining normal function of cartilage. Despite tremendous efforts in developing osteochondral plugs, formation of the osteochondral interface with proper zonal organization has not yet been reported. Here, we present a mesenchymal stem cell-collagen microsphere-based approach for complex tissue engineering and demonstrate in vitro formation of a stem cell-derived osteochondral interface with calcified cartilage interface separating a non-calcified cartilage layer and an underlying bone layer. Cells at the interface region are hypertrophic chondrocytes while the extracellular matrix in this region contains collagen type II and X, calcium deposits and vertically running fibers. The simultaneous presence of appropriate medium and configuration during co-culture is necessary for the interface formation. © 2010 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.subjectCalcified cartilageen_HK
dc.subjectCollagen microspheresen_HK
dc.subjectComplex tissue engineeringen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectMicroencapsulationen_HK
dc.subjectOsteochondral interfaceen_HK
dc.subject.meshCollagen - chemistry-
dc.subject.meshMesenchymal Stem Cells - cytology-
dc.subject.meshMicrospheres-
dc.subject.meshOsteogenesis - physiology-
dc.subject.meshTissue Engineering - methods-
dc.titleIn vitro generation of an osteochondral interface from mesenchymal stem cell-collagen microspheresen_HK
dc.typeArticleen_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2010.10.021en_HK
dc.identifier.pmid21093047-
dc.identifier.scopuseid_2-s2.0-78650293523en_HK
dc.identifier.hkuros196464en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650293523&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1526en_HK
dc.identifier.epage1535en_HK
dc.identifier.isiWOS:000286698100008-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridCheng, HW=35794981100en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.citeulike8371391-

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