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Article: Customized Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: Design, fabrication, surface modification and sustained release of growth factor

TitleCustomized Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: Design, fabrication, surface modification and sustained release of growth factor
Authors
KeywordsBone tissue engineering
Nanocomposite
Scaffold
Selective laser sintering
Surface modification
Issue Date2010
PublisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572
Citation
Journal Of The Royal Society Interface, 2010, v. 7 SUPPL. 5, p. S615-S629 How to Cite?
AbstractIntegrating an advanced manufacturing technique, nanocomposite material and controlled delivery of growth factor to form multifunctional tissue engineering scaffolds was investigated in this study. Based on calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite microspheres, three-dimensional Ca-P/PHBV nanocomposite scaffolds with customized architecture, controlled porosity and totally interconnected porous structure were successfully fabricated using selective laser sintering (SLS), one of the rapid prototyping technologies. The cytocompatibility of sintered Ca-P/PHBV nanocomposite scaffolds, as well as PHBV polymer scaffolds, was studied. For surface modification of nanocomposite scaffolds, gelatin was firstly physically entrapped onto the scaffold surface and heparin was subsequently immobilized on entrapped gelatin. The surface-modification improved the wettability of scaffolds and provided specific binding site between conjugated heparin and the growth factor recombinant human bone morphogenetic protein-2 (rhBMP-2). The surface-modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 significantly enhanced the alkaline phosphatase activity and osteogenic differentiation markers in gene expression of C3H10T1/2 mesenchymal stem cells. Together with osteoconductive nanocomposite material and controlled growth factor delivery strategies, the use of SLS technique to form complex scaffolds will provide a promising route towards individualized bone tissue regeneration. © 2010 The Royal Society.
Persistent Identifierhttp://hdl.handle.net/10722/139383
ISSN
2015 Impact Factor: 3.818
2015 SCImago Journal Rankings: 1.622
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong (HKU)
Hong Kong Research Grants CouncilHKU 7176/08E
Funding Information:

B.D. thanks the University of Hong Kong (HKU) for awarding him with the University Scholarship. This work was supported partly by a GRF grant (HKU 7176/08E) from the Hong Kong Research Grants Council and by a research grant from HKU. The authors thank Prof. Wai-Lam Cheung of HKU for his help in SLS, Dr Zhangyang Li and Prof. William W. Lu of HKU for their support in conducting the cell culture work, and Prof. Changsheng Liu of East China University of Science and Technology for providing rhBMP-2.

References

 

DC FieldValueLanguage
dc.contributor.authorDuan, Ben_HK
dc.contributor.authorWang, Men_HK
dc.date.accessioned2011-09-23T05:49:01Z-
dc.date.available2011-09-23T05:49:01Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of The Royal Society Interface, 2010, v. 7 SUPPL. 5, p. S615-S629en_HK
dc.identifier.issn1742-5689en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139383-
dc.description.abstractIntegrating an advanced manufacturing technique, nanocomposite material and controlled delivery of growth factor to form multifunctional tissue engineering scaffolds was investigated in this study. Based on calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite microspheres, three-dimensional Ca-P/PHBV nanocomposite scaffolds with customized architecture, controlled porosity and totally interconnected porous structure were successfully fabricated using selective laser sintering (SLS), one of the rapid prototyping technologies. The cytocompatibility of sintered Ca-P/PHBV nanocomposite scaffolds, as well as PHBV polymer scaffolds, was studied. For surface modification of nanocomposite scaffolds, gelatin was firstly physically entrapped onto the scaffold surface and heparin was subsequently immobilized on entrapped gelatin. The surface-modification improved the wettability of scaffolds and provided specific binding site between conjugated heparin and the growth factor recombinant human bone morphogenetic protein-2 (rhBMP-2). The surface-modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 significantly enhanced the alkaline phosphatase activity and osteogenic differentiation markers in gene expression of C3H10T1/2 mesenchymal stem cells. Together with osteoconductive nanocomposite material and controlled growth factor delivery strategies, the use of SLS technique to form complex scaffolds will provide a promising route towards individualized bone tissue regeneration. © 2010 The Royal Society.en_HK
dc.languageengen_US
dc.publisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572en_HK
dc.relation.ispartofJournal of the Royal Society Interfaceen_HK
dc.subjectBone tissue engineeringen_HK
dc.subjectNanocompositeen_HK
dc.subjectScaffolden_HK
dc.subjectSelective laser sinteringen_HK
dc.subjectSurface modificationen_HK
dc.subject.meshBone Morphogenetic Proteins - chemistry - metabolism-
dc.subject.meshCalcium Phosphates - chemistry-
dc.subject.meshMicrospheres-
dc.subject.meshNanocomposites - chemistry - ultrastructure-
dc.subject.meshTissue Scaffolds - chemistry-
dc.titleCustomized Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: Design, fabrication, surface modification and sustained release of growth factoren_HK
dc.typeArticleen_HK
dc.identifier.emailWang, M:memwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00185en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1098/rsif.2010.0127.focusen_HK
dc.identifier.pmid20504805-
dc.identifier.pmcidPMC3024573-
dc.identifier.scopuseid_2-s2.0-77957147532en_HK
dc.identifier.hkuros193970en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77957147532&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issueSUPPL. 5en_HK
dc.identifier.spageS615en_HK
dc.identifier.epageS629en_HK
dc.identifier.eissn1742-5662-
dc.identifier.isiWOS:000281773100008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDuan, B=7005042335en_HK
dc.identifier.scopusauthoridWang, M=15749714100en_HK

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