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Article: Encapsulation and release of biomolecules from Ca-P/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sintering

TitleEncapsulation and release of biomolecules from Ca-P/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sintering
Authors
KeywordsBovine serum albumin (BSA)
Calcium phosphate
Nanocomposite
Poly(hydroxybutyrate-co-hydroxyvalerate)
Selective laser sintering
Tissue engineering scaffold
Issue Date2010
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/polydegstab
Citation
Polymer Degradation And Stability, 2010, v. 95 n. 9, p. 1655-1664 How to Cite?
AbstractThis study focused on the fabrication of calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite scaffolds loaded with biomolecules using the selective laser sintering (SLS) technique and their evaluation. Ca-P/PHBV nanocomposite microspheres loaded with bovine serum albumin (BSA) as the model protein were fabricated using the double emulsion solvent evaporation method. The encapsulation efficiency of BSA in PHBV polymer microspheres and Ca-P/PHBV nanocomposite microspheres were 18.06 ± 0.86% and 24.51 ± 0.60%, respectively. The BSA loaded Ca-P/PHBV nanocomposite microspheres were successfully produced into three-dimensional porous scaffolds with good dimensional accuracy using the SLS technique. The nanocomposite microspheres served as protective carriers and maintained the bioactivity of BSA during SLS. The effects of SLS parameters such as laser power and scan spacing on the encapsulation efficiency of BSA in the scaffolds and in vitro BSA release were studied. An initial burst release was observed, which was followed by a slow release of BSA. After 28-day release, The PHBV matrix was slightly degraded after 28-day in vitro release study. It was shown that nanocomposite scaffolds with controlled architecture obtained via SLS could be incorporated with biomolecules, enhancing them with more functions for bone tissue engineering application or making them suitable for localized delivery of therapeutics. © 2010 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/139382
ISSN
2015 Impact Factor: 3.12
2015 SCImago Journal Rankings: 1.241
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong (HKU)
Hong Kong Research Grants CouncilHKU 7176/08E
Funding Information:

B. Duan thanks The University of Hong Kong (HKU) for making an award of the University Scholarship Award to him. This work was supported by a GRF grant (HKU 7176/08E) from the Hong Kong Research Grants Council and by a research grant from HKU. Assistance provided by technical staff in the Department of Mechanical Engineering, HKU, is acknowledged.

References

 

DC FieldValueLanguage
dc.contributor.authorDuan, Ben_HK
dc.contributor.authorWang, Men_HK
dc.date.accessioned2011-09-23T05:49:01Z-
dc.date.available2011-09-23T05:49:01Z-
dc.date.issued2010en_HK
dc.identifier.citationPolymer Degradation And Stability, 2010, v. 95 n. 9, p. 1655-1664en_HK
dc.identifier.issn0141-3910en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139382-
dc.description.abstractThis study focused on the fabrication of calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite scaffolds loaded with biomolecules using the selective laser sintering (SLS) technique and their evaluation. Ca-P/PHBV nanocomposite microspheres loaded with bovine serum albumin (BSA) as the model protein were fabricated using the double emulsion solvent evaporation method. The encapsulation efficiency of BSA in PHBV polymer microspheres and Ca-P/PHBV nanocomposite microspheres were 18.06 ± 0.86% and 24.51 ± 0.60%, respectively. The BSA loaded Ca-P/PHBV nanocomposite microspheres were successfully produced into three-dimensional porous scaffolds with good dimensional accuracy using the SLS technique. The nanocomposite microspheres served as protective carriers and maintained the bioactivity of BSA during SLS. The effects of SLS parameters such as laser power and scan spacing on the encapsulation efficiency of BSA in the scaffolds and in vitro BSA release were studied. An initial burst release was observed, which was followed by a slow release of BSA. After 28-day release, The PHBV matrix was slightly degraded after 28-day in vitro release study. It was shown that nanocomposite scaffolds with controlled architecture obtained via SLS could be incorporated with biomolecules, enhancing them with more functions for bone tissue engineering application or making them suitable for localized delivery of therapeutics. © 2010 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/polydegstaben_HK
dc.relation.ispartofPolymer Degradation and Stabilityen_HK
dc.subjectBovine serum albumin (BSA)en_HK
dc.subjectCalcium phosphateen_HK
dc.subjectNanocompositeen_HK
dc.subjectPoly(hydroxybutyrate-co-hydroxyvalerate)en_HK
dc.subjectSelective laser sinteringen_HK
dc.subjectTissue engineering scaffolden_HK
dc.titleEncapsulation and release of biomolecules from Ca-P/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sinteringen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, M:memwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00185en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.polymdegradstab.2010.05.022en_HK
dc.identifier.scopuseid_2-s2.0-77955512082en_HK
dc.identifier.hkuros193969en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955512082&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1655en_HK
dc.identifier.epage1664en_HK
dc.identifier.isiWOS:000281369500027-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDuan, B=7005042335en_HK
dc.identifier.scopusauthoridWang, M=15749714100en_HK
dc.identifier.citeulike7287375-

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