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Article: Assessment of glycosaminoglycan distribution in human lumbar intervertebral discs using chemical exchange saturation transfer at 3 T: Feasibility and initial experience

TitleAssessment of glycosaminoglycan distribution in human lumbar intervertebral discs using chemical exchange saturation transfer at 3 T: Feasibility and initial experience
Authors
KeywordsCEST
Glycosaminoglycan
Intervertebral disc
MRI
Issue Date2011
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087
Citation
Nmr In Biomedicine, 2011, v. 24 n. 9, p. 1137-1144 How to Cite?
AbstractRecent studies have proposed that glycosaminoglycan chemical exchange saturation transfer (gagCEST) is associated with a loss of glycosaminoglycans (GAGs), which may be an initiating factor in intervertebral disc (IVD) degeneration. Despite its promising potential, this application has not been reported in human in vivo IVD studies because of the challenges of B 0 magnetic field inhomogeneity in gagCEST. This study aimed to evaluate the feasibility of quantifying CEST values in IVDs of healthy volunteers using a clinical 3 T scanner. A single-slice turbo spin echo sequence was used to quantify the CEST effect in various GAG phantoms and in IVDs of 12 volunteers. The phantom results indicated high correlation between gagCEST and GAG concentrations (R 2=0.95). With optimal B 0 inhomogeneity correction, in vivo CEST maps of IVDs showed robust contrast between the nucleus pulposus (NP) and the annulus fibrosus (AF) (p<0.01), as well as higher signal in the central relative to the peripheral NP. In addition, a trend of decreasing CEST values from upper to lower disc levels was evident in NP. Our results demonstrate that in vivo gagCEST quantification in human lumbar IVDs is feasible at 3T in combination with successful B 0 inhomogeneity correction, but without significant hardware modifications. Our initial findings suggest that it would be worthwhile to perform direct correlation studies between CEST and GAGs using cadaver samples, and to extend this novel technique to studies on patients with degenerative discs to better understand its distinct imaging features relative to conventional techniques. Copyright © 2011 John Wiley & Sons, Ltd. The feasibility of in vivo glycosaminoglycan chemical exchange saturation transfer (gagCEST) quantification was investigated for the first time in human lumbar intervertebral discs (IVDs) using a clinical MRI scanner. Our data showed robust contrast between the nucleus pulposus (NP) and the annulus fibrosus, a higher signal in the central relative to peripheral NP and a trend of decreasing CEST values from upper to lower disc levels with successful B 0 inhomogeneity correction. Our initial findings suggest that it would be worthwhile to perform direct correlation studies between CEST and glycosaminoglycans using cadaver samples, and to extend this novel technique to studies on patients with degenerative discs to better understand its distinct imaging features relative to conventional techniques. © 2011 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/139132
ISSN
2015 Impact Factor: 2.983
2015 SCImago Journal Rankings: 1.533
ISI Accession Number ID
Funding AgencyGrant Number
General Research Fund
Research Grants Council of Hong Kong
Funding Information:

The authors wish to thank Professor Peter van Zijl and Mr Joseph Gillen (Johns Hopkins School of Medicine, Baltimore, MD, USA) for sharing a CEST pulse sequence and Dr Barbara Chan (University of Hong Kong) for producing GAG phantoms. This work was supported by a grant from the General Research Fund sponsored by the Research Grants Council of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorKim, Men_HK
dc.contributor.authorChan, Qen_HK
dc.contributor.authorAnthony, MPen_HK
dc.contributor.authorCheung, KMen_HK
dc.contributor.authorSamartzis, Den_HK
dc.contributor.authorKhong, PLen_HK
dc.date.accessioned2011-09-23T05:45:39Z-
dc.date.available2011-09-23T05:45:39Z-
dc.date.issued2011en_HK
dc.identifier.citationNmr In Biomedicine, 2011, v. 24 n. 9, p. 1137-1144en_HK
dc.identifier.issn0952-3480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139132-
dc.description.abstractRecent studies have proposed that glycosaminoglycan chemical exchange saturation transfer (gagCEST) is associated with a loss of glycosaminoglycans (GAGs), which may be an initiating factor in intervertebral disc (IVD) degeneration. Despite its promising potential, this application has not been reported in human in vivo IVD studies because of the challenges of B 0 magnetic field inhomogeneity in gagCEST. This study aimed to evaluate the feasibility of quantifying CEST values in IVDs of healthy volunteers using a clinical 3 T scanner. A single-slice turbo spin echo sequence was used to quantify the CEST effect in various GAG phantoms and in IVDs of 12 volunteers. The phantom results indicated high correlation between gagCEST and GAG concentrations (R 2=0.95). With optimal B 0 inhomogeneity correction, in vivo CEST maps of IVDs showed robust contrast between the nucleus pulposus (NP) and the annulus fibrosus (AF) (p<0.01), as well as higher signal in the central relative to the peripheral NP. In addition, a trend of decreasing CEST values from upper to lower disc levels was evident in NP. Our results demonstrate that in vivo gagCEST quantification in human lumbar IVDs is feasible at 3T in combination with successful B 0 inhomogeneity correction, but without significant hardware modifications. Our initial findings suggest that it would be worthwhile to perform direct correlation studies between CEST and GAGs using cadaver samples, and to extend this novel technique to studies on patients with degenerative discs to better understand its distinct imaging features relative to conventional techniques. Copyright © 2011 John Wiley & Sons, Ltd. The feasibility of in vivo glycosaminoglycan chemical exchange saturation transfer (gagCEST) quantification was investigated for the first time in human lumbar intervertebral discs (IVDs) using a clinical MRI scanner. Our data showed robust contrast between the nucleus pulposus (NP) and the annulus fibrosus, a higher signal in the central relative to peripheral NP and a trend of decreasing CEST values from upper to lower disc levels with successful B 0 inhomogeneity correction. Our initial findings suggest that it would be worthwhile to perform direct correlation studies between CEST and glycosaminoglycans using cadaver samples, and to extend this novel technique to studies on patients with degenerative discs to better understand its distinct imaging features relative to conventional techniques. © 2011 John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087en_HK
dc.relation.ispartofNMR in Biomedicineen_HK
dc.rightsNMR in Biomedicine. Copyright © John Wiley & Sons Ltd.-
dc.subjectCESTen_HK
dc.subjectGlycosaminoglycanen_HK
dc.subjectIntervertebral discen_HK
dc.subjectMRIen_HK
dc.subject.meshGlycosaminoglycans - metabolism-
dc.subject.meshIntervertebral Disc - metabolism-
dc.subject.meshLumbar Vertebrae - metabolism-
dc.subject.meshMagnetic Resonance Imaging - methods-
dc.subject.meshPhantoms, Imaging-
dc.titleAssessment of glycosaminoglycan distribution in human lumbar intervertebral discs using chemical exchange saturation transfer at 3 T: Feasibility and initial experienceen_HK
dc.typeArticleen_HK
dc.identifier.emailKim, M: minakim@hku.hken_HK
dc.identifier.emailAnthony, MP: anthonym@hku.hken_HK
dc.identifier.emailCheung, KM: cheungmc@hku.hken_HK
dc.identifier.emailSamartzis, D: dspine@hku.hken_HK
dc.identifier.emailKhong, PL: plkhong@hkucc.hku.hken_HK
dc.identifier.authorityKim, M=rp00292en_HK
dc.identifier.authorityAnthony, MP=rp01302en_HK
dc.identifier.authorityCheung, KM=rp00387en_HK
dc.identifier.authoritySamartzis, D=rp01430en_HK
dc.identifier.authorityKhong, PL=rp00467en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/nbm.1671en_HK
dc.identifier.pmid21387446-
dc.identifier.scopuseid_2-s2.0-80053197189en_HK
dc.identifier.hkuros192105en_US
dc.identifier.hkuros189132-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053197189&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1137en_HK
dc.identifier.epage1144en_HK
dc.identifier.isiWOS:000296419200014-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.f100013154958-
dc.identifier.scopusauthoridKim, M=8146283400en_HK
dc.identifier.scopusauthoridChan, Q=6602497305en_HK
dc.identifier.scopusauthoridAnthony, MP=35270974300en_HK
dc.identifier.scopusauthoridCheung, KM=7402406754en_HK
dc.identifier.scopusauthoridSamartzis, D=34572771100en_HK
dc.identifier.scopusauthoridKhong, PL=7006693233en_HK

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