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Article: Quantification of the lateral detachment force for bacterial cells using atomic force microscope and centrifugation

TitleQuantification of the lateral detachment force for bacterial cells using atomic force microscope and centrifugation
Authors
KeywordsAtomic force microscope
Centrifugation
Escherichia coli
Lateral detachment force
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ultramic
Citation
Ultramicroscopy, 2011, v. 111 n. 2, p. 131-139 How to Cite?
AbstractTo determine the lateral detachment force for individual bacterial cells, a quantitative method using the contact mode of an atomic force microscope (AFM) was developed in this study. Three key factors for the proposed method, i.e. scan size, scan rate and cantilever choice, were evaluated and optimized. The scan size of 40×40γm2 was optimal for capturing sufficient number of adhered cells in a microscopic field and provide adequate information for cell identification and detachment force measurement. The scan rate affected the measurement results significantly, and was optimized at 40γm/s considering both force measurement accuracy and experimental efficiency. The hardness of applied cantilevers also influenced force determination. The proposed protocol for cantilever selection is to use those with the lowest spring constant first and then step up to a harder cantilever until all cells are detached. The lateral detachment force of Escherichia coli cells on polished stainless steel and a glass-slide coated with poly-l-lysine were measured as 0.763±0.167 and 0.639±0.136nN, respectively. The results showed that the established method had good repeatability and sensitivity to various bacteria/substrata combinations. The detachment force quantified by AFM (0.639±0.136nN) was comparable to that measured by the centrifugation method (1.12nN). © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/139034
ISSN
2014 Impact Factor: 2.436
2013 SCImago Journal Rankings: 1.818
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong UGCSEG HKU10
HKU
Funding Information:

The authors wish to thank the Hong Kong UGC One-off Special Equipment Grant Scheme (SEG HKU10) for the financial support on this study, and Yuanqing Chao wishes to thank HKU for the postgraduate studentship. The technical assistance of Ms. Vicky Fung is greatly appreciated. The authors would also like to thank the reviewers for their comments.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorChao, Yen_HK
dc.contributor.authorShih, Ken_HK
dc.contributor.authorLi, XYen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2011-09-23T05:44:27Z-
dc.date.available2011-09-23T05:44:27Z-
dc.date.issued2011en_HK
dc.identifier.citationUltramicroscopy, 2011, v. 111 n. 2, p. 131-139en_HK
dc.identifier.issn0304-3991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139034-
dc.description.abstractTo determine the lateral detachment force for individual bacterial cells, a quantitative method using the contact mode of an atomic force microscope (AFM) was developed in this study. Three key factors for the proposed method, i.e. scan size, scan rate and cantilever choice, were evaluated and optimized. The scan size of 40×40γm2 was optimal for capturing sufficient number of adhered cells in a microscopic field and provide adequate information for cell identification and detachment force measurement. The scan rate affected the measurement results significantly, and was optimized at 40γm/s considering both force measurement accuracy and experimental efficiency. The hardness of applied cantilevers also influenced force determination. The proposed protocol for cantilever selection is to use those with the lowest spring constant first and then step up to a harder cantilever until all cells are detached. The lateral detachment force of Escherichia coli cells on polished stainless steel and a glass-slide coated with poly-l-lysine were measured as 0.763±0.167 and 0.639±0.136nN, respectively. The results showed that the established method had good repeatability and sensitivity to various bacteria/substrata combinations. The detachment force quantified by AFM (0.639±0.136nN) was comparable to that measured by the centrifugation method (1.12nN). © 2010 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ultramicen_HK
dc.relation.ispartofUltramicroscopyen_HK
dc.subjectAtomic force microscopeen_HK
dc.subjectCentrifugationen_HK
dc.subjectEscherichia colien_HK
dc.subjectLateral detachment forceen_HK
dc.subject.meshBacterial Adhesion-
dc.subject.meshCentrifugation - methods-
dc.subject.meshEscherichia coli K12 - physiology-
dc.subject.meshMicroscopy, Atomic Force - methods-
dc.subject.meshReproducibility of Results-
dc.titleQuantification of the lateral detachment force for bacterial cells using atomic force microscope and centrifugationen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailShih, K:kshih@hkucc.hku.hken_HK
dc.identifier.emailLi, XY:xlia@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityShih, K=rp00167en_HK
dc.identifier.authorityLi, XY=rp00222en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ultramic.2010.10.005en_HK
dc.identifier.pmid21185457en_HK
dc.identifier.scopuseid_2-s2.0-78449233654en_HK
dc.identifier.hkuros192699en_US
dc.identifier.hkuros208094-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78449233654&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume111en_HK
dc.identifier.issue2en_HK
dc.identifier.spage131en_HK
dc.identifier.epage139en_HK
dc.identifier.isiWOS:000286552200007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridChao, Y=36503486900en_HK
dc.identifier.scopusauthoridShih, K=14072108900en_HK
dc.identifier.scopusauthoridLi, XY=26642887900en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.citeulike8297634-

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