Article: Estimation of nitrifier abundances in a partial nitrification reactor treating ammonium-rich saline wastewater using DGGE, T-RFLP and mathematical modeling
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- Publisher Website: 10.1007/s00253-010-2837-3
- Scopus: eid_2-s2.0-78649329735
- PMID: 20737268
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| Title | Estimation of nitrifier abundances in a partial nitrification reactor treating ammonium-rich saline wastewater using DGGE, T-RFLP and mathematical modeling |
|---|---|
| Authors | Ye, L1 Zhang, T1 |
| Keywords | DGGE Double Monod Partial nitrification T-RFLP |
| Issue Date | 2010 |
| Publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm |
| Citation | Applied Microbiology And Biotechnology, 2010, v. 88 n. 6, p. 1403-1412 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-010-2837-3 |
| Abstract | The bacterial community in a partial nitrification reactor was analyzed on the basis of 16S rRNA gene by cloning-sequencing method, and the percentages of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the activated sludge were quantified by three independent methods, namely, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and Double Monod modeling. The clone library results suggested that there were only a dominant AOB and a dominant NOB species in the reactor, belonging to Nitrosomonas genus and Nitrospira genus, respectively. The percentages of NOB in total bacterial community increased from almost 0% to 30% when dissolved oxygen (DO) levels were changed from 0.15 mg/L to 0.5 mg/L, coinciding with the accumulation and conversion of nitrite, while the percentages of AOB changed little in the two phases. The results confirmed the importance of low DO level for inhibiting NOB to achieve partial nitrification. Furthermore, the percentages of AOB and NOB in the total bacteria community were estimated based on the results of batch experiments using Double Monod model, and the results were comparable with those determined according to profiles of DGGE and T-RFLP. © 2010 Springer-Verlag. |
| ISSN | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 |
| DOI | http://dx.doi.org/10.1007/s00253-010-2837-3 |
| References | References in Scopus |
| dc.contributor.author | Ye, L | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, T | ||||||
| dc.date.accessioned | 2011-09-23T05:44:27Z | ||||||
| dc.date.available | 2011-09-23T05:44:27Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | The bacterial community in a partial nitrification reactor was analyzed on the basis of 16S rRNA gene by cloning-sequencing method, and the percentages of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the activated sludge were quantified by three independent methods, namely, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and Double Monod modeling. The clone library results suggested that there were only a dominant AOB and a dominant NOB species in the reactor, belonging to Nitrosomonas genus and Nitrospira genus, respectively. The percentages of NOB in total bacterial community increased from almost 0% to 30% when dissolved oxygen (DO) levels were changed from 0.15 mg/L to 0.5 mg/L, coinciding with the accumulation and conversion of nitrite, while the percentages of AOB changed little in the two phases. The results confirmed the importance of low DO level for inhibiting NOB to achieve partial nitrification. Furthermore, the percentages of AOB and NOB in the total bacteria community were estimated based on the results of batch experiments using Double Monod model, and the results were comparable with those determined according to profiles of DGGE and T-RFLP. © 2010 Springer-Verlag. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Applied Microbiology And Biotechnology, 2010, v. 88 n. 6, p. 1403-1412 [How to Cite?] DOI: http://dx.doi.org/10.1007/s00253-010-2837-3 | ||||||
| dc.identifier.citeulike | 7816637 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1007/s00253-010-2837-3 | ||||||
| dc.identifier.epage | 1412 | ||||||
| dc.identifier.hkuros | 192697 | ||||||
| dc.identifier.isi | WOS:000284267700019
Funding Information: The authors wish to thank the Hong Kong General Research Fund (HKU7197/08E) for the financial support of this study, and Lin Ye wishes to thank HKU for the postgraduate studentship. | ||||||
| dc.identifier.issn | 0175-7598 2011 Impact Factor: 3.425 2011 SCImago Journal Rankings: 0.180 | ||||||
| dc.identifier.issue | 6 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 20737268 | ||||||
| dc.identifier.scopus | eid_2-s2.0-78649329735 | ||||||
| dc.identifier.spage | 1403 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/139032 | ||||||
| dc.identifier.volume | 88 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Springer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm | ||||||
| dc.publisher.place | Germany | ||||||
| dc.relation.ispartof | Applied Microbiology and Biotechnology | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.rights | The original publication is available at www.springerlink.com | ||||||
| dc.subject.mesh | Bacteria - classification - genetics - metabolism | ||||||
| dc.subject.mesh | Biodiversity | ||||||
| dc.subject.mesh | Bioreactors - microbiology | ||||||
| dc.subject.mesh | Nitrification | ||||||
| dc.subject.mesh | Quaternary Ammonium Compounds - metabolism | ||||||
| dc.subject | DGGE | ||||||
| dc.subject | Double Monod | ||||||
| dc.subject | Partial nitrification | ||||||
| dc.subject | T-RFLP | ||||||
| dc.title | Estimation of nitrifier abundances in a partial nitrification reactor treating ammonium-rich saline wastewater using DGGE, T-RFLP and mathematical modeling | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong