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- Publisher Website: 10.1016/j.niox.2011.01.009
- Scopus: eid_2-s2.0-79954585869
- PMID: 21296177
- WOS: WOS:000294275700023
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Article: Detection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response
Title | Detection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response |
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Authors | |
Keywords | Arabidopsis thaliana Avirulent Pseudomonas syringae Hypersensitive response Peroxynitrite Tyrosine nitration Urate |
Issue Date | 2011 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yniox |
Citation | Nitric Oxide - Biology And Chemistry, 2011, v. 25 n. 2, p. 222-228 How to Cite? |
Abstract | Nitric oxide (NO) is synthesized in plants in response to stress, and its role in signaling is well-documented. In contrast, very little is known about the physiological role of its derivate peroxynitrite (ONOO -), which forms when NO reacts with O2- and induces protein modification by tyrosine nitration. Infection with an avirulent pathogen triggers the simultaneous production of NO and reactive oxygen species, as well as an increase in tyrosine nitration, so peroxynitrite could be physiologically relevant during this process. To gain insight into the role of peroxynitrite in plants, we measured its accumulation during the hypersensitive response in Arabidopsis thaliana using the specific peroxynitrite-sensitive fluorescent dye HKGreen-2 in a leaf disc assay. The avirulent pathogen Pseudomonas syringae pv. tomato, carrying the AvrB gene (Pst AvrB), induced a strong increase in fluorescence 3-4 h post-infiltration (hpi) which peaked 7-8 hpi. The increase in HKGreen-2 fluorescence was inhibited by co-injecting the peroxynitrite-scavenger urate together with the pathogen, and was almost completely eliminated by co-infiltrating urate with HKGreen-2, confirming that HKGreen-2 fluorescence in planta is induced specifically by peroxynitrite. This establishes a link between peroxynitrite synthesis and tyrosine nitration, and we therefore propose that peroxynitrite transduces the NO signal by modifying protein functions. © 2010 Published by Elsevier Inc. |
Persistent Identifier | http://hdl.handle.net/10722/139024 |
ISSN | 2021 Impact Factor: 4.898 2020 SCImago Journal Rankings: 1.040 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Gaupels, F | en_HK |
dc.contributor.author | SpiazziVandelle, E | en_HK |
dc.contributor.author | Yang, D | en_HK |
dc.contributor.author | Delledonne, M | en_HK |
dc.date.accessioned | 2011-09-23T05:44:11Z | - |
dc.date.available | 2011-09-23T05:44:11Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Nitric Oxide - Biology And Chemistry, 2011, v. 25 n. 2, p. 222-228 | en_HK |
dc.identifier.issn | 1089-8603 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/139024 | - |
dc.description.abstract | Nitric oxide (NO) is synthesized in plants in response to stress, and its role in signaling is well-documented. In contrast, very little is known about the physiological role of its derivate peroxynitrite (ONOO -), which forms when NO reacts with O2- and induces protein modification by tyrosine nitration. Infection with an avirulent pathogen triggers the simultaneous production of NO and reactive oxygen species, as well as an increase in tyrosine nitration, so peroxynitrite could be physiologically relevant during this process. To gain insight into the role of peroxynitrite in plants, we measured its accumulation during the hypersensitive response in Arabidopsis thaliana using the specific peroxynitrite-sensitive fluorescent dye HKGreen-2 in a leaf disc assay. The avirulent pathogen Pseudomonas syringae pv. tomato, carrying the AvrB gene (Pst AvrB), induced a strong increase in fluorescence 3-4 h post-infiltration (hpi) which peaked 7-8 hpi. The increase in HKGreen-2 fluorescence was inhibited by co-injecting the peroxynitrite-scavenger urate together with the pathogen, and was almost completely eliminated by co-infiltrating urate with HKGreen-2, confirming that HKGreen-2 fluorescence in planta is induced specifically by peroxynitrite. This establishes a link between peroxynitrite synthesis and tyrosine nitration, and we therefore propose that peroxynitrite transduces the NO signal by modifying protein functions. © 2010 Published by Elsevier Inc. | en_HK |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yniox | en_HK |
dc.relation.ispartof | Nitric Oxide - Biology and Chemistry | en_HK |
dc.rights | NOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI# | en_US |
dc.subject | Arabidopsis thaliana | en_HK |
dc.subject | Avirulent Pseudomonas syringae | en_HK |
dc.subject | Hypersensitive response | en_HK |
dc.subject | Peroxynitrite | en_HK |
dc.subject | Tyrosine nitration | en_HK |
dc.subject | Urate | en_HK |
dc.title | Detection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1089-8603&volume=25&spage=222&epage=228&date=2011&atitle=Detection+of+peroxynitrite+accumulation+in+Arabidopsis+thaliana+during+the+hypersensitive+defense+response | en_US |
dc.identifier.email | Yang, D:yangdan@hku.hk | en_HK |
dc.identifier.authority | Yang, D=rp00825 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.niox.2011.01.009 | en_HK |
dc.identifier.pmid | 21296177 | - |
dc.identifier.scopus | eid_2-s2.0-79954585869 | en_HK |
dc.identifier.hkuros | 196071 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79954585869&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 25 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 222 | en_HK |
dc.identifier.epage | 228 | en_HK |
dc.identifier.isi | WOS:000294275700023 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Gaupels, F=24072860400 | en_HK |
dc.identifier.scopusauthorid | SpiazziVandelle, E=37004936700 | en_HK |
dc.identifier.scopusauthorid | Yang, D=7404800756 | en_HK |
dc.identifier.scopusauthorid | Delledonne, M=6602083954 | en_HK |
dc.identifier.citeulike | 8790183 | - |
dc.identifier.issnl | 1089-8603 | - |