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Article: Detection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response

TitleDetection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense response
Authors
KeywordsArabidopsis thaliana
Avirulent Pseudomonas syringae
Hypersensitive response
Peroxynitrite
Tyrosine nitration
Urate
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yniox
Citation
Nitric Oxide - Biology And Chemistry, 2011, v. 25 n. 2, p. 222-228 How to Cite?
AbstractNitric oxide (NO) is synthesized in plants in response to stress, and its role in signaling is well-documented. In contrast, very little is known about the physiological role of its derivate peroxynitrite (ONOO -), which forms when NO reacts with O2- and induces protein modification by tyrosine nitration. Infection with an avirulent pathogen triggers the simultaneous production of NO and reactive oxygen species, as well as an increase in tyrosine nitration, so peroxynitrite could be physiologically relevant during this process. To gain insight into the role of peroxynitrite in plants, we measured its accumulation during the hypersensitive response in Arabidopsis thaliana using the specific peroxynitrite-sensitive fluorescent dye HKGreen-2 in a leaf disc assay. The avirulent pathogen Pseudomonas syringae pv. tomato, carrying the AvrB gene (Pst AvrB), induced a strong increase in fluorescence 3-4 h post-infiltration (hpi) which peaked 7-8 hpi. The increase in HKGreen-2 fluorescence was inhibited by co-injecting the peroxynitrite-scavenger urate together with the pathogen, and was almost completely eliminated by co-infiltrating urate with HKGreen-2, confirming that HKGreen-2 fluorescence in planta is induced specifically by peroxynitrite. This establishes a link between peroxynitrite synthesis and tyrosine nitration, and we therefore propose that peroxynitrite transduces the NO signal by modifying protein functions. © 2010 Published by Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/139024
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.292
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGaupels, Fen_HK
dc.contributor.authorSpiazziVandelle, Een_HK
dc.contributor.authorYang, Den_HK
dc.contributor.authorDelledonne, Men_HK
dc.date.accessioned2011-09-23T05:44:11Z-
dc.date.available2011-09-23T05:44:11Z-
dc.date.issued2011en_HK
dc.identifier.citationNitric Oxide - Biology And Chemistry, 2011, v. 25 n. 2, p. 222-228en_HK
dc.identifier.issn1089-8603en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139024-
dc.description.abstractNitric oxide (NO) is synthesized in plants in response to stress, and its role in signaling is well-documented. In contrast, very little is known about the physiological role of its derivate peroxynitrite (ONOO -), which forms when NO reacts with O2- and induces protein modification by tyrosine nitration. Infection with an avirulent pathogen triggers the simultaneous production of NO and reactive oxygen species, as well as an increase in tyrosine nitration, so peroxynitrite could be physiologically relevant during this process. To gain insight into the role of peroxynitrite in plants, we measured its accumulation during the hypersensitive response in Arabidopsis thaliana using the specific peroxynitrite-sensitive fluorescent dye HKGreen-2 in a leaf disc assay. The avirulent pathogen Pseudomonas syringae pv. tomato, carrying the AvrB gene (Pst AvrB), induced a strong increase in fluorescence 3-4 h post-infiltration (hpi) which peaked 7-8 hpi. The increase in HKGreen-2 fluorescence was inhibited by co-injecting the peroxynitrite-scavenger urate together with the pathogen, and was almost completely eliminated by co-infiltrating urate with HKGreen-2, confirming that HKGreen-2 fluorescence in planta is induced specifically by peroxynitrite. This establishes a link between peroxynitrite synthesis and tyrosine nitration, and we therefore propose that peroxynitrite transduces the NO signal by modifying protein functions. © 2010 Published by Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ynioxen_HK
dc.relation.ispartofNitric Oxide - Biology and Chemistryen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#en_US
dc.subjectArabidopsis thalianaen_HK
dc.subjectAvirulent Pseudomonas syringaeen_HK
dc.subjectHypersensitive responseen_HK
dc.subjectPeroxynitriteen_HK
dc.subjectTyrosine nitrationen_HK
dc.subjectUrateen_HK
dc.titleDetection of peroxynitrite accumulation in Arabidopsis thaliana during the hypersensitive defense responseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1089-8603&volume=25&spage=222&epage=228&date=2011&atitle=Detection+of+peroxynitrite+accumulation+in+Arabidopsis+thaliana+during+the+hypersensitive+defense+responseen_US
dc.identifier.emailYang, D:yangdan@hku.hken_HK
dc.identifier.authorityYang, D=rp00825en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.niox.2011.01.009en_HK
dc.identifier.pmid21296177-
dc.identifier.scopuseid_2-s2.0-79954585869en_HK
dc.identifier.hkuros196071en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79954585869&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue2en_HK
dc.identifier.spage222en_HK
dc.identifier.epage228en_HK
dc.identifier.isiWOS:000294275700023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGaupels, F=24072860400en_HK
dc.identifier.scopusauthoridSpiazziVandelle, E=37004936700en_HK
dc.identifier.scopusauthoridYang, D=7404800756en_HK
dc.identifier.scopusauthoridDelledonne, M=6602083954en_HK
dc.identifier.citeulike8790183-

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