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Article: Correlation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiation

TitleCorrelation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiation
Authors
Issue Date2011
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
Citation
Tissue Engineering - Part A, 2011, v. 17 n. 5-6, p. 777-788 How to Cite?
AbstractMesenchymal stem cell (MSC)-based engineering is promising for cartilage repair. However, the compositional mechanical relationship of the engineered structures has not been extensively studied, given the importance of such relationship in native cartilage tissues. In this study, a novel human MSC-collagen microsphere system was used to study the compositional mechanical relationship during in vitro chondrogenic differentiation using histological and biochemical methods and a microplate compression assay. The mechanical property was found positively correlating with newly deposited cartilage-relevant matrices, glycosaminoglycan, and type II collagen, and with the collagen crosslinker density, in agreement with the presence of thick collagen bundles upon structural characterization. On the other hand, the mechanical property negatively correlates with type I collagen and total collagen, suggesting that the initial collagen matrix scaffold of the microsphere system was being remodeled by the differentiating human MSCs. This study also demonstrated the application of a simple, sensitive, and nondestructive tool for monitoring the progression of chondrogenic differentiation of MSCs in tissue-engineered constructs and therefore contributes to future development of novel cartilage repair strategies. © Mary Ann Liebert, Inc. 2011.
Persistent Identifierhttp://hdl.handle.net/10722/138971
ISSN
2015 SCImago Journal Rankings: 1.500
ISI Accession Number ID
Funding AgencyGrant Number
ITC, Hong Kong GovernmentGHP/050/06
University Research Committee
University of Hong Kong10206799
Strategic Research Theme on Nanotechnology
Funding Information:

This work was supported by ITF Fund, ITC, Hong Kong Government (GHP/050/06), University Research Committee, the University of Hong Kong (No: 10206799), and the Strategic Research Theme on Nanotechnology. The authors thank Dr. G. C. F. Chan and Mr. S. Chen for providing the MSCs.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLi, CHen_HK
dc.contributor.authorChik, TKen_HK
dc.contributor.authorNgan, AHWen_HK
dc.contributor.authorChan, SCHen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2011-09-23T05:43:21Z-
dc.date.available2011-09-23T05:43:21Z-
dc.date.issued2011en_HK
dc.identifier.citationTissue Engineering - Part A, 2011, v. 17 n. 5-6, p. 777-788en_HK
dc.identifier.issn1937-3341en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138971-
dc.description.abstractMesenchymal stem cell (MSC)-based engineering is promising for cartilage repair. However, the compositional mechanical relationship of the engineered structures has not been extensively studied, given the importance of such relationship in native cartilage tissues. In this study, a novel human MSC-collagen microsphere system was used to study the compositional mechanical relationship during in vitro chondrogenic differentiation using histological and biochemical methods and a microplate compression assay. The mechanical property was found positively correlating with newly deposited cartilage-relevant matrices, glycosaminoglycan, and type II collagen, and with the collagen crosslinker density, in agreement with the presence of thick collagen bundles upon structural characterization. On the other hand, the mechanical property negatively correlates with type I collagen and total collagen, suggesting that the initial collagen matrix scaffold of the microsphere system was being remodeled by the differentiating human MSCs. This study also demonstrated the application of a simple, sensitive, and nondestructive tool for monitoring the progression of chondrogenic differentiation of MSCs in tissue-engineered constructs and therefore contributes to future development of novel cartilage repair strategies. © Mary Ann Liebert, Inc. 2011.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263en_HK
dc.relation.ispartofTissue Engineering - Part Aen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Tissue Engineering - Part A] © [2011] [copyright Mary Ann Liebert, Inc.]; [Tissue Engineering - Part A] is available online at: http://www.liebertonline.com.-
dc.subject.meshCell Differentiation - drug effectsen_US
dc.subject.meshChondrogenesis - drug effectsen_US
dc.subject.meshCollagen - pharmacologyen_US
dc.subject.meshMesenchymal Stem Cells - cytology - drug effects - metabolism - ultrastructureen_US
dc.subject.meshMicrospheresen_US
dc.titleCorrelation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-3341&volume=17&issue=5-6&spage=777&epage=788&date=2011&atitle=Correlation+between+compositional+and+mechanical+properties+of+human+mesenchymal+stem+cell-collagen+microspheres+during+chondrogenic+differentiationen_US
dc.identifier.emailNgan, AHW:hwngan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityNgan, AHW=rp00225en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/ten.tea.2010.0078en_HK
dc.identifier.pmid20964578en_US
dc.identifier.scopuseid_2-s2.0-79952177196en_HK
dc.identifier.hkuros196466en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952177196&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage777en_HK
dc.identifier.epage788en_HK
dc.identifier.isiWOS:000287801600017-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectCollagen biomaterial and bone marrow derived mesenchymal stem cell (MSCs) based therapy - Second generation tissue engineering solutions for cartilage repair-
dc.identifier.scopusauthoridLi, CH=37034415100en_HK
dc.identifier.scopusauthoridChik, TK=37033578800en_HK
dc.identifier.scopusauthoridNgan, AHW=7006827202en_HK
dc.identifier.scopusauthoridChan, SCH=35261745200en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

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