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Article: The regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells

TitleThe regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells
Authors
KeywordsBiomaterials
Bone marrow stromal cell
Chitosan
Nerve regeneration
Nerve tissue engineering
Peripheral nerve injury
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2011, v. 32 n. 3, p. 787-796 How to Cite?
AbstractAutologous nerve grafts have been the 'gold standard' for treatment of peripheral nerve defects that exceed the critical gap length. To address issues of limited availability of donor nerves and donor site morbidity, we have fabricated chitosan conduits and seeded them with bone marrow stromal cell (BMSC)-derived Schwann cells as an alternative. The derived Schwann cells used were checked for fate commitment. The conduits were tested for efficacy in bridging the critical gap length of 12 mm in sciatic nerves of adult rats. By three months post-operation, mid-shank circumference, nerve conduction velocity, average regenerated myelin area, and myelinated axon count, in nerves bridged with BMSC-derived Schwann cells were similar to those treated with sciatic nerve-derived Schwann cells (p > 0.05) but significantly higher than those bridged with PBS-filled conduits (p < 0.05). Evidence is thus provided in support of the use of chitosan conduits seeded with BMSC-derived Schwann cells to treat critical defects in peripheral nerves. This provides the basis to pursue BMSC as an autologous source of Schwann cells for transplantation therapy in larger animal species. © 2010 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/138969
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Dr Cheng Yu-Tung Fellowship
LKS Faculty of Medicine The University of Hong Kong
Hong Kong Research Grants CouncilGRF 777810
Funding Information:

This work was supported by the Strategic Research Theme of The University of Hong Kong the Dr Cheng Yu-Tung Fellowship Scheme LKS Faculty of Medicine The University of Hong Kong and the Hong Kong Research Grants Council GRF 777810

References

 

DC FieldValueLanguage
dc.contributor.authorAo, Qen_HK
dc.contributor.authorFung, CKen_HK
dc.contributor.authorYatPing Tsui, Aen_HK
dc.contributor.authorCai, Sen_HK
dc.contributor.authorZuo, HCen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorKwokYan Shum, Den_HK
dc.date.accessioned2011-09-23T05:43:20Z-
dc.date.available2011-09-23T05:43:20Z-
dc.date.issued2011en_HK
dc.identifier.citationBiomaterials, 2011, v. 32 n. 3, p. 787-796en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138969-
dc.description.abstractAutologous nerve grafts have been the 'gold standard' for treatment of peripheral nerve defects that exceed the critical gap length. To address issues of limited availability of donor nerves and donor site morbidity, we have fabricated chitosan conduits and seeded them with bone marrow stromal cell (BMSC)-derived Schwann cells as an alternative. The derived Schwann cells used were checked for fate commitment. The conduits were tested for efficacy in bridging the critical gap length of 12 mm in sciatic nerves of adult rats. By three months post-operation, mid-shank circumference, nerve conduction velocity, average regenerated myelin area, and myelinated axon count, in nerves bridged with BMSC-derived Schwann cells were similar to those treated with sciatic nerve-derived Schwann cells (p > 0.05) but significantly higher than those bridged with PBS-filled conduits (p < 0.05). Evidence is thus provided in support of the use of chitosan conduits seeded with BMSC-derived Schwann cells to treat critical defects in peripheral nerves. This provides the basis to pursue BMSC as an autologous source of Schwann cells for transplantation therapy in larger animal species. © 2010 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.subjectBiomaterialsen_HK
dc.subjectBone marrow stromal cellen_HK
dc.subjectChitosanen_HK
dc.subjectNerve regenerationen_HK
dc.subjectNerve tissue engineeringen_HK
dc.subjectPeripheral nerve injuryen_HK
dc.subject.meshBone Marrow Cells - cytologyen_US
dc.subject.meshChitosan - chemistryen_US
dc.subject.meshGuided Tissue Regeneration - methodsen_US
dc.subject.meshMesenchymal Stem Cells - cytologyen_US
dc.subject.meshNerve Regeneration - physiologyen_US
dc.titleThe regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailKwokYan Shum, D: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityKwokYan Shum, D=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2010.09.046en_HK
dc.identifier.pmid20950852-
dc.identifier.scopuseid_2-s2.0-78549265105en_HK
dc.identifier.hkuros195175en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78549265105&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue3en_HK
dc.identifier.spage787en_HK
dc.identifier.epage796en_HK
dc.identifier.eissn1878-5905-
dc.identifier.isiWOS:000285322600014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridAo, Q=8891582700en_HK
dc.identifier.scopusauthoridFung, CK=37037255400en_HK
dc.identifier.scopusauthoridYatPing Tsui, A=36545498300en_HK
dc.identifier.scopusauthoridCai, S=21740464700en_HK
dc.identifier.scopusauthoridZuo, HC=7005708907en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridKwokYan Shum, D=7004824447en_HK
dc.identifier.citeulike8041292-

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