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Article: Proteomic analysis of intestinal ischemia/reperfusion injury and ischemic preconditioning in rats reveals the protective role of aldose reductase

TitleProteomic analysis of intestinal ischemia/reperfusion injury and ischemic preconditioning in rats reveals the protective role of aldose reductase
Authors
KeywordsAldose reductase
Animal proteomics
Ischemic preconditioning
Rat
Reperfusion injury
Issue Date2010
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2010, v. 10 n. 24, p. 4463-4475 How to Cite?
AbstractIntestinal ischemia/reperfusion (I/R) injury is a critical condition associated with high morbidity and mortality. Studies show that ischemic preconditioning (IPC) can protect the intestine from I/R injury. However, the underlying molecular mechanisms of this event have not been fully elucidated. In the present study, 2-DE combined with MALDI-MS was employed to analyze intestinal mucosa proteomes of rat subjected to I/R injury in the absence or presence of IPC pretreatment. The protein content of 16 proteins in the intestinal mucosa changed more than 1.5-fold following intestinal I/R. These proteins were, respectively, involved in the cellular processes of energy metabolism, anti-oxidation and anti-apoptosis. One of these proteins, aldose reductase (AR), removes reactive oxygen species. In support of the 2-DE results, the mRNA and protein expressions of AR were significantly downregulated upon I/R injury and enhanced by IPC as confirmed by RT-PCR and western blot analysis. Further study showed that AR-selective inhibitor epalrestat totally turned over the protective effect of IPC, indicating that IPC confers protection against intestinal I/R injury primarily by increasing intestinal AR expression. The finding that AR may play a key in intestinal ischemic protection might offer evidences to foster the development of new therapies against intestinal I/R injury. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/138928
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30672021
30872446
Funding Information:

The authors thank Dr. Liao Dong-Jiang for technical help in proteomic analysis. This work was supported by a grant from National Natural Science Foundation of China (No: 30672021, 30872446 to Ke-Xuan Liu).

References
Errata

 

DC FieldValueLanguage
dc.contributor.authorLiu, KXen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorLi, YSen_HK
dc.contributor.authorYuan, BLen_HK
dc.contributor.authorXu, Men_HK
dc.contributor.authorXia, Zen_HK
dc.contributor.authorHuang, WQen_HK
dc.date.accessioned2011-09-23T05:42:15Z-
dc.date.available2011-09-23T05:42:15Z-
dc.date.issued2010en_HK
dc.identifier.citationProteomics, 2010, v. 10 n. 24, p. 4463-4475en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138928-
dc.description.abstractIntestinal ischemia/reperfusion (I/R) injury is a critical condition associated with high morbidity and mortality. Studies show that ischemic preconditioning (IPC) can protect the intestine from I/R injury. However, the underlying molecular mechanisms of this event have not been fully elucidated. In the present study, 2-DE combined with MALDI-MS was employed to analyze intestinal mucosa proteomes of rat subjected to I/R injury in the absence or presence of IPC pretreatment. The protein content of 16 proteins in the intestinal mucosa changed more than 1.5-fold following intestinal I/R. These proteins were, respectively, involved in the cellular processes of energy metabolism, anti-oxidation and anti-apoptosis. One of these proteins, aldose reductase (AR), removes reactive oxygen species. In support of the 2-DE results, the mRNA and protein expressions of AR were significantly downregulated upon I/R injury and enhanced by IPC as confirmed by RT-PCR and western blot analysis. Further study showed that AR-selective inhibitor epalrestat totally turned over the protective effect of IPC, indicating that IPC confers protection against intestinal I/R injury primarily by increasing intestinal AR expression. The finding that AR may play a key in intestinal ischemic protection might offer evidences to foster the development of new therapies against intestinal I/R injury. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectAldose reductaseen_HK
dc.subjectAnimal proteomicsen_HK
dc.subjectIschemic preconditioningen_HK
dc.subjectRaten_HK
dc.subjectReperfusion injuryen_HK
dc.subject.meshAldehyde Reductase - physiology-
dc.subject.meshIntestinal Mucosa - immunology - metabolism - pathology-
dc.subject.meshIntestine, Small - blood supply - metabolism-
dc.subject.meshIschemic Preconditioning-
dc.subject.meshProteome - metabolism-
dc.titleProteomic analysis of intestinal ischemia/reperfusion injury and ischemic preconditioning in rats reveals the protective role of aldose reductaseen_HK
dc.typeArticleen_HK
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.201000078en_HK
dc.identifier.pmid21136599-
dc.identifier.scopuseid_2-s2.0-78650050186en_HK
dc.identifier.hkuros193417en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650050186&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue24en_HK
dc.identifier.spage4463en_HK
dc.identifier.epage4475en_HK
dc.identifier.isiWOS:000285882200012-
dc.publisher.placeGermanyen_HK
dc.relation.erratumdoi:10.1002/pmic.201090113-

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