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Article: TRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits
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TitleTRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits
 
AuthorsWang, M2 3
Ji, P3
Wang, R3
Zhao, L3
Xia, Z1
 
Keywordscapsaicin
ischemia-reperfusion
lung
TRPV1
 
Issue Date2012
 
PublisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsre
 
CitationJournal Of Surgical Research, 2012, v. 173 n. 1, p. 153-160 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jss.2010.08.053
 
AbstractBackground: Capsaicin, a transient receptor potential vanilloid type1 (TRPV1) agonist, was found to protect against myocardial and renal ischemia-reperfusion (IR) injury. This study was carried out to investigate the role of capsaicin in lung IR injury in vivo. Methods: Forty male New Zealand rabbits were randomized into four groups (10 per group) as follows: sham group (sham thoracotomy), IR group (occlusion of the left pulmonary hilus for 1 h followed by reperfusion for 3 h), CAP (capsaicin) group (a bolus injection of CAP 5 min before ischemia), CPZ (capsazepine) group (a bolus injection of the TRPV1 antagonist CPZ 5 min before ischemia). Blood and lung tissue samples were obtained for blood gas and biochemical analyses, wet/dry weight ratio measurements, and histologic evaluation. Protein levels and neutrophils in the bronchoalveolar lavage fluid (BALF) were also measured. Results: Pretreatment with capsaicin improved gas exchange function, decreased lung wet/dry ratio and protein levels and neutrophil counts in BALF, decreased lung malondialdehyde levels and myeloperoxidase activities, increased superoxide dismutase activities, along with an elevation of calcitonin gene-related peptide (CGRP) level (P < 0.05 versus IR group). Capsaicin also attenuated IR-induced pathological lesions. By contrast, capsazepine exacerbated gas exchange abnormality, increased pulmonary microvascular permeability, oxidative stress, neutrophils infiltration, and also revealed a decreased CGRP level (P < 0.05 versus IR group). Conclusion: Results from the present study show that capsaicin confers protection against lung IR injury. These protective effects seem to be closely related to the inhibition of inflammation and oxidative stress via the activation of TRPV1 and the release of CGRP. © 2012 Elsevier Inc. All rights reserved.
 
ISSN0022-4804
2013 Impact Factor: 2.121
 
DOIhttp://dx.doi.org/10.1016/j.jss.2010.08.053
 
ISI Accession Number IDWOS:000300612500030
Funding AgencyGrant Number
National Natural Science Foundation of China30672260
81170077
Funding Information:

The author thanks Nanfu Luo, Daqing Liao, and Chaoran Wu for their technical support. This work was supported by National Natural Science Foundation of China (30672260 and 81170077).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, M
 
dc.contributor.authorJi, P
 
dc.contributor.authorWang, R
 
dc.contributor.authorZhao, L
 
dc.contributor.authorXia, Z
 
dc.date.accessioned2011-09-23T05:42:14Z
 
dc.date.available2011-09-23T05:42:14Z
 
dc.date.issued2012
 
dc.description.abstractBackground: Capsaicin, a transient receptor potential vanilloid type1 (TRPV1) agonist, was found to protect against myocardial and renal ischemia-reperfusion (IR) injury. This study was carried out to investigate the role of capsaicin in lung IR injury in vivo. Methods: Forty male New Zealand rabbits were randomized into four groups (10 per group) as follows: sham group (sham thoracotomy), IR group (occlusion of the left pulmonary hilus for 1 h followed by reperfusion for 3 h), CAP (capsaicin) group (a bolus injection of CAP 5 min before ischemia), CPZ (capsazepine) group (a bolus injection of the TRPV1 antagonist CPZ 5 min before ischemia). Blood and lung tissue samples were obtained for blood gas and biochemical analyses, wet/dry weight ratio measurements, and histologic evaluation. Protein levels and neutrophils in the bronchoalveolar lavage fluid (BALF) were also measured. Results: Pretreatment with capsaicin improved gas exchange function, decreased lung wet/dry ratio and protein levels and neutrophil counts in BALF, decreased lung malondialdehyde levels and myeloperoxidase activities, increased superoxide dismutase activities, along with an elevation of calcitonin gene-related peptide (CGRP) level (P < 0.05 versus IR group). Capsaicin also attenuated IR-induced pathological lesions. By contrast, capsazepine exacerbated gas exchange abnormality, increased pulmonary microvascular permeability, oxidative stress, neutrophils infiltration, and also revealed a decreased CGRP level (P < 0.05 versus IR group). Conclusion: Results from the present study show that capsaicin confers protection against lung IR injury. These protective effects seem to be closely related to the inhibition of inflammation and oxidative stress via the activation of TRPV1 and the release of CGRP. © 2012 Elsevier Inc. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Surgical Research, 2012, v. 173 n. 1, p. 153-160 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jss.2010.08.053
 
dc.identifier.citeulike7936766
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jss.2010.08.053
 
dc.identifier.epage160
 
dc.identifier.hkuros193414
 
dc.identifier.isiWOS:000300612500030
Funding AgencyGrant Number
National Natural Science Foundation of China30672260
81170077
Funding Information:

The author thanks Nanfu Luo, Daqing Liao, and Chaoran Wu for their technical support. This work was supported by National Natural Science Foundation of China (30672260 and 81170077).

 
dc.identifier.issn0022-4804
2013 Impact Factor: 2.121
 
dc.identifier.issue1
 
dc.identifier.pmid20950828
 
dc.identifier.scopuseid_2-s2.0-84857038055
 
dc.identifier.spage153
 
dc.identifier.urihttp://hdl.handle.net/10722/138927
 
dc.identifier.volume173
 
dc.languageeng
 
dc.publisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsre
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Surgical Research
 
dc.relation.referencesReferences in Scopus
 
dc.subjectcapsaicin
 
dc.subjectischemia-reperfusion
 
dc.subjectlung
 
dc.subjectTRPV1
 
dc.titleTRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits
 
dc.typeArticle
 
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<contributor.author>Zhao, L</contributor.author>
<contributor.author>Xia, Z</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Luzhou Medical College
  3. Sichuan University