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Article: TRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits

TitleTRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbits
Authors
Keywordscapsaicin
ischemia-reperfusion
lung
TRPV1
Issue Date2012
PublisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsre
Citation
Journal Of Surgical Research, 2012, v. 173 n. 1, p. 153-160 How to Cite?
Abstract
Background: Capsaicin, a transient receptor potential vanilloid type1 (TRPV1) agonist, was found to protect against myocardial and renal ischemia-reperfusion (IR) injury. This study was carried out to investigate the role of capsaicin in lung IR injury in vivo. Methods: Forty male New Zealand rabbits were randomized into four groups (10 per group) as follows: sham group (sham thoracotomy), IR group (occlusion of the left pulmonary hilus for 1 h followed by reperfusion for 3 h), CAP (capsaicin) group (a bolus injection of CAP 5 min before ischemia), CPZ (capsazepine) group (a bolus injection of the TRPV1 antagonist CPZ 5 min before ischemia). Blood and lung tissue samples were obtained for blood gas and biochemical analyses, wet/dry weight ratio measurements, and histologic evaluation. Protein levels and neutrophils in the bronchoalveolar lavage fluid (BALF) were also measured. Results: Pretreatment with capsaicin improved gas exchange function, decreased lung wet/dry ratio and protein levels and neutrophil counts in BALF, decreased lung malondialdehyde levels and myeloperoxidase activities, increased superoxide dismutase activities, along with an elevation of calcitonin gene-related peptide (CGRP) level (P < 0.05 versus IR group). Capsaicin also attenuated IR-induced pathological lesions. By contrast, capsazepine exacerbated gas exchange abnormality, increased pulmonary microvascular permeability, oxidative stress, neutrophils infiltration, and also revealed a decreased CGRP level (P < 0.05 versus IR group). Conclusion: Results from the present study show that capsaicin confers protection against lung IR injury. These protective effects seem to be closely related to the inhibition of inflammation and oxidative stress via the activation of TRPV1 and the release of CGRP. © 2012 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/138927
ISSN
2013 Impact Factor: 2.121
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30672260
81170077
Funding Information:

The author thanks Nanfu Luo, Daqing Liao, and Chaoran Wu for their technical support. This work was supported by National Natural Science Foundation of China (30672260 and 81170077).

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Luzhou Medical College
  3. Sichuan University
DC FieldValueLanguage
dc.contributor.authorWang, Men_HK
dc.contributor.authorJi, Pen_HK
dc.contributor.authorWang, Ren_HK
dc.contributor.authorZhao, Len_HK
dc.contributor.authorXia, Zen_HK
dc.date.accessioned2011-09-23T05:42:14Z-
dc.date.available2011-09-23T05:42:14Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Surgical Research, 2012, v. 173 n. 1, p. 153-160en_HK
dc.identifier.issn0022-4804en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138927-
dc.description.abstractBackground: Capsaicin, a transient receptor potential vanilloid type1 (TRPV1) agonist, was found to protect against myocardial and renal ischemia-reperfusion (IR) injury. This study was carried out to investigate the role of capsaicin in lung IR injury in vivo. Methods: Forty male New Zealand rabbits were randomized into four groups (10 per group) as follows: sham group (sham thoracotomy), IR group (occlusion of the left pulmonary hilus for 1 h followed by reperfusion for 3 h), CAP (capsaicin) group (a bolus injection of CAP 5 min before ischemia), CPZ (capsazepine) group (a bolus injection of the TRPV1 antagonist CPZ 5 min before ischemia). Blood and lung tissue samples were obtained for blood gas and biochemical analyses, wet/dry weight ratio measurements, and histologic evaluation. Protein levels and neutrophils in the bronchoalveolar lavage fluid (BALF) were also measured. Results: Pretreatment with capsaicin improved gas exchange function, decreased lung wet/dry ratio and protein levels and neutrophil counts in BALF, decreased lung malondialdehyde levels and myeloperoxidase activities, increased superoxide dismutase activities, along with an elevation of calcitonin gene-related peptide (CGRP) level (P < 0.05 versus IR group). Capsaicin also attenuated IR-induced pathological lesions. By contrast, capsazepine exacerbated gas exchange abnormality, increased pulmonary microvascular permeability, oxidative stress, neutrophils infiltration, and also revealed a decreased CGRP level (P < 0.05 versus IR group). Conclusion: Results from the present study show that capsaicin confers protection against lung IR injury. These protective effects seem to be closely related to the inhibition of inflammation and oxidative stress via the activation of TRPV1 and the release of CGRP. © 2012 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Inc.. The Journal's web site is located at http://www.elsevier.com/locate/jsreen_HK
dc.relation.ispartofJournal of Surgical Researchen_HK
dc.subjectcapsaicinen_HK
dc.subjectischemia-reperfusionen_HK
dc.subjectlungen_HK
dc.subjectTRPV1en_HK
dc.titleTRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury in rabbitsen_HK
dc.typeArticleen_HK
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jss.2010.08.053en_HK
dc.identifier.pmid20950828-
dc.identifier.scopuseid_2-s2.0-84857038055en_HK
dc.identifier.hkuros193414en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857038055&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume173en_HK
dc.identifier.issue1en_HK
dc.identifier.spage153en_HK
dc.identifier.epage160en_HK
dc.identifier.isiWOS:000300612500030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike7936766-

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