Article: Reaction of silver diamine fluoride with hydroxyapatite and protein
| Title | Reaction of silver diamine fluoride with hydroxyapatite and protein | ||||
|---|---|---|---|---|---|
| Authors | Lou, YL2 Botelho, MG2 Darvell, BW1 | ||||
| Keywords | Fluoride Hydroxyapatite Protein Silver diamine fluoride | ||||
| Issue Date | 2011 | ||||
| Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent | ||||
| Citation | Journal Of Dentistry, 2011, v. 39 n. 9, p. 612-618 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jdent.2011.06.008 | ||||
| Abstract | Silver diammine fluoride (SDF) is used as an anticaries agent; however, its mode of action is uncertain, whether chemical, physical, mechanical or antibacterial. As a preliminary study, the effect of SDF on hydroxyapatite (HAp) and gelatin (as a chemically-representative protein) was examined. Methods: 2.5 mg HAp powder specimens and 0.5 mL 10% gelatin (Riedel-de Haën) (initially as a sol at ∼37 °C), were mixed with 0.5 mL of 38% SDF (J. Morita), 4% NaF (Sigma) or 40% AgNO 3 (Sigma) and tumbled in 1.5 mL polypropylene tubes (Sarstedt) for 48 h at ∼23 °C, in two series: exposed to laboratory lighting, and kept dark at all times. The HAp specimens were separated by centrifugation and decanting, then these and one set of gelatin specimens were dried at 60 °C in situ; a second parallel set of gelatin specimens were dried at ∼23 °C. Each was washed with 1 mL deionized water for 1 min, 3 times. Treated materials were observed, before and after washing, with scanning and transmission electron microscopy (SEM, TEM); energy-dispersive X-ray analysis (EDX), and electron diffraction (ED). Results: SDF appeared to produce globular particles of CaF 2 on the surface of the HAp, but these disappeared on washing, whilst with AgNO 3 yellow cubic crystals of Ag 3PO 4 formed which were not dissolved on washing, but which darkened, converting gradually to metallic silver, on exposure to light. NaF had no effect on gelatin, whilst with SDF and AgNO 3, particles of silver were produced which were resistant to washing. Conclusions: Both principal components of tooth tissue react with SDF; the solubility of the putative CaF 2 formed weakens the case for it exerting a caries-protective effect. The importance of the persistent silver needs further study. © 2011 Elsevier Ltd. All rights reserved. | ||||
| ISSN | 0300-5712 2011 Impact Factor: 2.947 2011 SCImago Journal Rankings: 0.097 | ||||
| DOI | http://dx.doi.org/10.1016/j.jdent.2011.06.008 | ||||
| ISI Accession Number ID | WOS:000294455700004
Funding Information: This work was done in partial fulfilment of the requirements for the degree of Ph.D. for YaLi Lou at and supported by the Faculty of Dentistry, The University of Hong Kong. The authors thank HaoBo Pan for the supply of the HAp; Amy S. L. Wong and W. S. Lee for assistance with the SEM and EDX observations; and Frankie Y.F. Chan for help with the TEM observations. | ||||
| References | References in Scopus |
| dc.contributor.author | Lou, YL | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Botelho, MG | ||||
| dc.contributor.author | Darvell, BW | ||||
| dc.date.accessioned | 2011-09-23T05:41:37Z | ||||
| dc.date.available | 2011-09-23T05:41:37Z | ||||
| dc.date.issued | 2011 | ||||
| dc.description.abstract | Silver diammine fluoride (SDF) is used as an anticaries agent; however, its mode of action is uncertain, whether chemical, physical, mechanical or antibacterial. As a preliminary study, the effect of SDF on hydroxyapatite (HAp) and gelatin (as a chemically-representative protein) was examined. Methods: 2.5 mg HAp powder specimens and 0.5 mL 10% gelatin (Riedel-de Haën) (initially as a sol at ∼37 °C), were mixed with 0.5 mL of 38% SDF (J. Morita), 4% NaF (Sigma) or 40% AgNO 3 (Sigma) and tumbled in 1.5 mL polypropylene tubes (Sarstedt) for 48 h at ∼23 °C, in two series: exposed to laboratory lighting, and kept dark at all times. The HAp specimens were separated by centrifugation and decanting, then these and one set of gelatin specimens were dried at 60 °C in situ; a second parallel set of gelatin specimens were dried at ∼23 °C. Each was washed with 1 mL deionized water for 1 min, 3 times. Treated materials were observed, before and after washing, with scanning and transmission electron microscopy (SEM, TEM); energy-dispersive X-ray analysis (EDX), and electron diffraction (ED). Results: SDF appeared to produce globular particles of CaF 2 on the surface of the HAp, but these disappeared on washing, whilst with AgNO 3 yellow cubic crystals of Ag 3PO 4 formed which were not dissolved on washing, but which darkened, converting gradually to metallic silver, on exposure to light. NaF had no effect on gelatin, whilst with SDF and AgNO 3, particles of silver were produced which were resistant to washing. Conclusions: Both principal components of tooth tissue react with SDF; the solubility of the putative CaF 2 formed weakens the case for it exerting a caries-protective effect. The importance of the persistent silver needs further study. © 2011 Elsevier Ltd. All rights reserved. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Journal Of Dentistry, 2011, v. 39 n. 9, p. 612-618 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jdent.2011.06.008 | ||||
| dc.identifier.citeulike | 9531727 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.jdent.2011.06.008 | ||||
| dc.identifier.epage | 618 | ||||
| dc.identifier.hkuros | 194701 | ||||
| dc.identifier.isi | WOS:000294455700004
Funding Information: This work was done in partial fulfilment of the requirements for the degree of Ph.D. for YaLi Lou at and supported by the Faculty of Dentistry, The University of Hong Kong. The authors thank HaoBo Pan for the supply of the HAp; Amy S. L. Wong and W. S. Lee for assistance with the SEM and EDX observations; and Frankie Y.F. Chan for help with the TEM observations. | ||||
| dc.identifier.issn | 0300-5712 2011 Impact Factor: 2.947 2011 SCImago Journal Rankings: 0.097 | ||||
| dc.identifier.issue | 9 | ||||
| dc.identifier.pmid | 21745530 | ||||
| dc.identifier.scopus | eid_2-s2.0-80051473020 | ||||
| dc.identifier.spage | 612 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/138880 | ||||
| dc.identifier.volume | 39 | ||||
| dc.language | eng | ||||
| dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent | ||||
| dc.publisher.place | United Kingdom | ||||
| dc.relation.ispartof | Journal of Dentistry | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject.mesh | Durapatite - chemistry | ||||
| dc.subject.mesh | Gelatin - chemistry | ||||
| dc.subject.mesh | Microscopy, Electron, Transmission | ||||
| dc.subject.mesh | Quaternary Ammonium Compounds - chemistry | ||||
| dc.subject.mesh | Spectrometry, X-Ray Emission | ||||
| dc.subject | Fluoride | ||||
| dc.subject | Hydroxyapatite | ||||
| dc.subject | Protein | ||||
| dc.subject | Silver diamine fluoride | ||||
| dc.title | Reaction of silver diamine fluoride with hydroxyapatite and protein | ||||
| dc.type | Article |
Author Affiliations
- University of Kuwait
- The University of Hong Kong