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Article: Reaction of silver diamine fluoride with hydroxyapatite and protein

TitleReaction of silver diamine fluoride with hydroxyapatite and protein
Authors
KeywordsFluoride
Hydroxyapatite
Protein
Silver diamine fluoride
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2011, v. 39 n. 9, p. 612-618 How to Cite?
AbstractSilver diammine fluoride (SDF) is used as an anticaries agent; however, its mode of action is uncertain, whether chemical, physical, mechanical or antibacterial. As a preliminary study, the effect of SDF on hydroxyapatite (HAp) and gelatin (as a chemically-representative protein) was examined. Methods: 2.5 mg HAp powder specimens and 0.5 mL 10% gelatin (Riedel-de Haën) (initially as a sol at ∼37 °C), were mixed with 0.5 mL of 38% SDF (J. Morita), 4% NaF (Sigma) or 40% AgNO 3 (Sigma) and tumbled in 1.5 mL polypropylene tubes (Sarstedt) for 48 h at ∼23 °C, in two series: exposed to laboratory lighting, and kept dark at all times. The HAp specimens were separated by centrifugation and decanting, then these and one set of gelatin specimens were dried at 60 °C in situ; a second parallel set of gelatin specimens were dried at ∼23 °C. Each was washed with 1 mL deionized water for 1 min, 3 times. Treated materials were observed, before and after washing, with scanning and transmission electron microscopy (SEM, TEM); energy-dispersive X-ray analysis (EDX), and electron diffraction (ED). Results: SDF appeared to produce globular particles of CaF 2 on the surface of the HAp, but these disappeared on washing, whilst with AgNO 3 yellow cubic crystals of Ag 3PO 4 formed which were not dissolved on washing, but which darkened, converting gradually to metallic silver, on exposure to light. NaF had no effect on gelatin, whilst with SDF and AgNO 3, particles of silver were produced which were resistant to washing. Conclusions: Both principal components of tooth tissue react with SDF; the solubility of the putative CaF 2 formed weakens the case for it exerting a caries-protective effect. The importance of the persistent silver needs further study. © 2011 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/138880
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID
Funding AgencyGrant Number
Faculty of Dentistry, The University of Hong Kong
Funding Information:

This work was done in partial fulfilment of the requirements for the degree of Ph.D. for YaLi Lou at and supported by the Faculty of Dentistry, The University of Hong Kong. The authors thank HaoBo Pan for the supply of the HAp; Amy S. L. Wong and W. S. Lee for assistance with the SEM and EDX observations; and Frankie Y.F. Chan for help with the TEM observations.

References

 

DC FieldValueLanguage
dc.contributor.authorLou, YLen_HK
dc.contributor.authorBotelho, MGen_HK
dc.contributor.authorDarvell, BWen_HK
dc.date.accessioned2011-09-23T05:41:37Z-
dc.date.available2011-09-23T05:41:37Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Dentistry, 2011, v. 39 n. 9, p. 612-618en_HK
dc.identifier.issn0300-5712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138880-
dc.description.abstractSilver diammine fluoride (SDF) is used as an anticaries agent; however, its mode of action is uncertain, whether chemical, physical, mechanical or antibacterial. As a preliminary study, the effect of SDF on hydroxyapatite (HAp) and gelatin (as a chemically-representative protein) was examined. Methods: 2.5 mg HAp powder specimens and 0.5 mL 10% gelatin (Riedel-de Haën) (initially as a sol at ∼37 °C), were mixed with 0.5 mL of 38% SDF (J. Morita), 4% NaF (Sigma) or 40% AgNO 3 (Sigma) and tumbled in 1.5 mL polypropylene tubes (Sarstedt) for 48 h at ∼23 °C, in two series: exposed to laboratory lighting, and kept dark at all times. The HAp specimens were separated by centrifugation and decanting, then these and one set of gelatin specimens were dried at 60 °C in situ; a second parallel set of gelatin specimens were dried at ∼23 °C. Each was washed with 1 mL deionized water for 1 min, 3 times. Treated materials were observed, before and after washing, with scanning and transmission electron microscopy (SEM, TEM); energy-dispersive X-ray analysis (EDX), and electron diffraction (ED). Results: SDF appeared to produce globular particles of CaF 2 on the surface of the HAp, but these disappeared on washing, whilst with AgNO 3 yellow cubic crystals of Ag 3PO 4 formed which were not dissolved on washing, but which darkened, converting gradually to metallic silver, on exposure to light. NaF had no effect on gelatin, whilst with SDF and AgNO 3, particles of silver were produced which were resistant to washing. Conclusions: Both principal components of tooth tissue react with SDF; the solubility of the putative CaF 2 formed weakens the case for it exerting a caries-protective effect. The importance of the persistent silver needs further study. © 2011 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_HK
dc.relation.ispartofJournal of Dentistryen_HK
dc.subjectFluorideen_HK
dc.subjectHydroxyapatiteen_HK
dc.subjectProteinen_HK
dc.subjectSilver diamine fluorideen_HK
dc.subject.meshDurapatite - chemistry-
dc.subject.meshGelatin - chemistry-
dc.subject.meshMicroscopy, Electron, Transmission-
dc.subject.meshQuaternary Ammonium Compounds - chemistry-
dc.subject.meshSpectrometry, X-Ray Emission-
dc.titleReaction of silver diamine fluoride with hydroxyapatite and proteinen_HK
dc.typeArticleen_HK
dc.identifier.emailBotelho, MG: botelho@hkucc.hku.hken_HK
dc.identifier.emailDarvell, BW: b.w.darvell@hku.hken_HK
dc.identifier.authorityBotelho, MG=rp00033en_HK
dc.identifier.authorityDarvell, BW=rp00007en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jdent.2011.06.008en_HK
dc.identifier.pmid21745530-
dc.identifier.scopuseid_2-s2.0-80051473020en_HK
dc.identifier.hkuros194701en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80051473020&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue9en_HK
dc.identifier.spage612en_HK
dc.identifier.epage618en_HK
dc.identifier.eissn1879-176X-
dc.identifier.isiWOS:000294455700004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLou, YL=48461581100en_HK
dc.identifier.scopusauthoridBotelho, MG=7005751169en_HK
dc.identifier.scopusauthoridDarvell, BW=7005953926en_HK
dc.identifier.citeulike9531727-

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