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Article: Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars
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TitleUse of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars
 
AuthorsChan, YL1
Ngan, AHW1
King, NM1
 
Issue Date2009
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#description
 
CitationJournal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jmbbm.2009.01.006
 
AbstractIn this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved.
 
ISSN1751-6161
2013 Impact Factor: 3.048
2013 SCImago Journal Rankings: 1.131
 
DOIhttp://dx.doi.org/10.1016/j.jmbbm.2009.01.006
 
ISI Accession Number IDWOS:000266833700009
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, PR ChinaHKU7156/08E
University of Hong Kong0208242.16180.14500
Funding Information:

This investigation was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, PR China (Project No.HKU7156/08E), as well as a grant from the University of Hong Kong (Project No. 10208242.16180.14500). We are grateful to Mr. S.C.K. Chan, Mr. P.W.L. Wong and Mr. K.O. Lam of the Department of orthopedics and Traumatology for their assistance in processing of the samples. We are also grateful to colleagues from the Medical Engineering Laboratory for their kind assistance in storage of the dental samples and the three-point flexural tests.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, YL
 
dc.contributor.authorNgan, AHW
 
dc.contributor.authorKing, NM
 
dc.date.accessioned2011-09-23T05:41:32Z
 
dc.date.available2011-09-23T05:41:32Z
 
dc.date.issued2009
 
dc.description.abstractIn this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jmbbm.2009.01.006
 
dc.identifier.citeulike9177880
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jmbbm.2009.01.006
 
dc.identifier.epage383
 
dc.identifier.hkuros193245
 
dc.identifier.hkuros155562
 
dc.identifier.isiWOS:000266833700009
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, PR ChinaHKU7156/08E
University of Hong Kong0208242.16180.14500
Funding Information:

This investigation was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, PR China (Project No.HKU7156/08E), as well as a grant from the University of Hong Kong (Project No. 10208242.16180.14500). We are grateful to Mr. S.C.K. Chan, Mr. P.W.L. Wong and Mr. K.O. Lam of the Department of orthopedics and Traumatology for their assistance in processing of the samples. We are also grateful to colleagues from the Medical Engineering Laboratory for their kind assistance in storage of the dental samples and the three-point flexural tests.

 
dc.identifier.issn1751-6161
2013 Impact Factor: 3.048
2013 SCImago Journal Rankings: 1.131
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid19627844
 
dc.identifier.scopuseid_2-s2.0-67349086269
 
dc.identifier.spage375
 
dc.identifier.urihttp://hdl.handle.net/10722/138870
 
dc.identifier.volume2
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#description
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofJournal of the Mechanical Behavior of Biomedical Materials
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBiomechanics
 
dc.subject.meshCalcification, Physiologic
 
dc.subject.meshDental Enamel - physiology
 
dc.subject.meshMolar - anatomy and histology - physiology
 
dc.subject.meshNanotechnology
 
dc.titleUse of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong