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Article: Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars

TitleUse of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars
Authors
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#description
Citation
Journal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383 How to Cite?
AbstractIn this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/138870
ISSN
2015 Impact Factor: 2.876
2015 SCImago Journal Rankings: 1.068
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, PR ChinaHKU7156/08E
University of Hong Kong0208242.16180.14500
Funding Information:

This investigation was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, PR China (Project No.HKU7156/08E), as well as a grant from the University of Hong Kong (Project No. 10208242.16180.14500). We are grateful to Mr. S.C.K. Chan, Mr. P.W.L. Wong and Mr. K.O. Lam of the Department of orthopedics and Traumatology for their assistance in processing of the samples. We are also grateful to colleagues from the Medical Engineering Laboratory for their kind assistance in storage of the dental samples and the three-point flexural tests.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, YLen_HK
dc.contributor.authorNgan, AHWen_HK
dc.contributor.authorKing, NMen_HK
dc.date.accessioned2011-09-23T05:41:32Z-
dc.date.available2011-09-23T05:41:32Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383en_HK
dc.identifier.issn1751-6161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138870-
dc.description.abstractIn this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#descriptionen_HK
dc.relation.ispartofJournal of the Mechanical Behavior of Biomedical Materialsen_HK
dc.subject.meshBiomechanics-
dc.subject.meshCalcification, Physiologic-
dc.subject.meshDental Enamel - physiology-
dc.subject.meshMolar - anatomy and histology - physiology-
dc.subject.meshNanotechnology-
dc.titleUse of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molarsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1751-6161&volume=2&issue=4&spage=375&epage=383&date=2009&atitle=Use+of+focused+ion+beam+milling+for+investigating+the+mechanical+properties+of+biological+tissues:+a+study+of+human+primary+molars-
dc.identifier.emailNgan, AHW: hwngan@hkucc.hku.hken_HK
dc.identifier.emailKing, NM: hhdbknm@hkucc.hku.hken_HK
dc.identifier.authorityNgan, AHW=rp00225en_HK
dc.identifier.authorityKing, NM=rp00006en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jmbbm.2009.01.006en_HK
dc.identifier.pmid19627844-
dc.identifier.scopuseid_2-s2.0-67349086269en_HK
dc.identifier.hkuros193245en_US
dc.identifier.hkuros155562-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349086269&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2en_HK
dc.identifier.issue4en_HK
dc.identifier.spage375en_HK
dc.identifier.epage383en_HK
dc.identifier.isiWOS:000266833700009-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, YL=8250546500en_HK
dc.identifier.scopusauthoridNgan, AHW=7006827202en_HK
dc.identifier.scopusauthoridKing, NM=7201762850en_HK
dc.identifier.citeulike9177880-

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