Article: Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars
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- Publisher Website: 10.1016/j.jmbbm.2009.01.006
- Scopus: eid_2-s2.0-67349086269
- PMID: 19627844
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| Title | Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars |
|---|---|
| Authors | Chan, YL1 Ngan, AHW1 King, NM1 |
| Issue Date | 2009 |
| Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#description |
| Citation | Journal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jmbbm.2009.01.006 |
| Abstract | In this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved. |
| ISSN | 1751-6161 2011 Impact Factor: 2.814 2011 SCImago Journal Rankings: 0.222 |
| DOI | http://dx.doi.org/10.1016/j.jmbbm.2009.01.006 |
| References | References in Scopus |
| dc.contributor.author | Chan, YL | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Ngan, AHW | ||||||
| dc.contributor.author | King, NM | ||||||
| dc.date.accessioned | 2011-09-23T05:41:32Z | ||||||
| dc.date.available | 2011-09-23T05:41:32Z | ||||||
| dc.date.issued | 2009 | ||||||
| dc.description.abstract | In this paper, the usefulness of the specimen shaping ability of focused ion beam (FIB) milling in the micrometer scale and the high force resolution of the nanoindentation technique are demonstrated on human primary teeth. Micro-cantilevers, with a triangular cross-section <5 μm in width and 10 μm in length, were produced within 50 μm of the dentin-enamel junction (DEJ) using FIB milling, and were point-loaded at their free ends at 20 μN/s until failure using a nanoindenter. The elastic modulus and flexural strength of such micro-samples of human enamel, and their variation with respect to prism orientation, were studied and compared to data from bulk enamel measured using nanoindentation and three-point bend tests. The elastic modulus of the micro-cantilever samples was found to be comparable to that obtained by nanoindentation on bulk samples, but it demonstrated significant anisotropy commensurate with the microstructure of enamel which was not measurable using nanoindentation on bulk samples. The flexural strength of the enamel micro-cantilevers also exhibited strong anisotropy, and was about one order of magnitude higher than that of bulk specimens measured by three-point bending. Through a Weibull analysis, this size dependence of the strength was found to be similar to the normal behaviour in brittle materials. The flexural strength of the enamel samples was also found to be sensitive to changes in the degree of mineralization of the samples. © 2009 Elsevier Ltd. All rights reserved. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of The Mechanical Behavior Of Biomedical Materials, 2009, v. 2 n. 4, p. 375-383 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.jmbbm.2009.01.006 | ||||||
| dc.identifier.citeulike | 9177880 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.jmbbm.2009.01.006 | ||||||
| dc.identifier.epage | 383 | ||||||
| dc.identifier.hkuros | 193245 | ||||||
| dc.identifier.hkuros | 155562 | ||||||
| dc.identifier.isi | WOS:000266833700009
Funding Information: This investigation was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, PR China (Project No.HKU7156/08E), as well as a grant from the University of Hong Kong (Project No. 10208242.16180.14500). We are grateful to Mr. S.C.K. Chan, Mr. P.W.L. Wong and Mr. K.O. Lam of the Department of orthopedics and Traumatology for their assistance in processing of the samples. We are also grateful to colleagues from the Medical Engineering Laboratory for their kind assistance in storage of the dental samples and the three-point flexural tests. | ||||||
| dc.identifier.issn | 1751-6161 2011 Impact Factor: 2.814 2011 SCImago Journal Rankings: 0.222 | ||||||
| dc.identifier.issue | 4 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 19627844 | ||||||
| dc.identifier.scopus | eid_2-s2.0-67349086269 | ||||||
| dc.identifier.spage | 375 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/138870 | ||||||
| dc.identifier.volume | 2 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/711005/description#description | ||||||
| dc.publisher.place | Netherlands | ||||||
| dc.relation.ispartof | Journal of the Mechanical Behavior of Biomedical Materials | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Biomechanics | ||||||
| dc.subject.mesh | Calcification, Physiologic | ||||||
| dc.subject.mesh | Dental Enamel - physiology | ||||||
| dc.subject.mesh | Molar - anatomy and histology - physiology | ||||||
| dc.subject.mesh | Nanotechnology | ||||||
| dc.title | Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong