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Article: Evidence for bias in C/N, δ13C and δ15N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methods

TitleEvidence for bias in C/N, δ13C and δ15N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methods
Authors
Keywordsδ13C
δ15N
C/N ratios
Environmental interpretation
Organic matter
Palaeoclimate
Pre-analysis acid treatment methods
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/quascirev
Citation
Quaternary Science Reviews, 2011, v. 30 n. 21-22, p. 3076-3087 How to Cite?
AbstractThere is a known bias in C/N, δ 13C and δ 15N values of organic matter (OM) due to pre-analysis acid treatment methods. We report here, for the first time, the results of a pre-analysis acid treatment method comparison of measured C/N, δ 13C and δ 15N values in bulk OM from a sedimentary sequence of samples to illustrate this bias. Here we show that acid treatment significantly reduces the accuracy (between method biases) and precision (within method bias) of C/N, δ 13C and δ 15N values of OM, suggesting a differential response of sample OM between methods and sample horizons, and in some cases inefficient removal of inorganic carbon. We show that different methods can significantly influence environmental interpretation in some of our sample horizons (i.e. interpretation of aquatic vs. terrestrial OM source; C 3 vs. C 4 vegetation). Specifically, there are unpredictable and non-linear differences between methods for C/N values in the range of ∼1-100; δ 13C values in the range of 0.2-6.8‰ and; δ 15N values in the range of 0.3-0.7‰. Importantly, these ranges are mostly much greater than the instrument precision (defined as the standard deviation of replicate analysis of standard reference materials; for this study, ±0.5 for C/N values, ±0.1‰ for δ 13C values and; ±0.1‰ for δ 15N). The accuracy and precision of measured C/N, δ 13C and δ 15N values of bulk OM is not just dependent upon environmental variability, but on acid pre-treatment, residual inorganic carbon and organic matter state and composition. Collectively, this makes the correlation between samples prepared in different ways, including those from down-core reconstructions, highly questionable. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/138795
ISSN
2015 Impact Factor: 4.521
2015 SCImago Journal Rankings: 2.928
ISI Accession Number ID
Funding AgencyGrant Number
NERCNE/F007264/1
IP/1165/0510
1423.1009
Funding Information:

CRB acknowledges support from the NERC through PhD studentship NE/F007264/1, through NERC Isotope Facility Grant IP/1165/0510 (to JML) and a NERC Radiocarbon Facility Grant 1423.1009 (to JML).

References

 

DC FieldValueLanguage
dc.contributor.authorBrodie, CRen_HK
dc.contributor.authorCasford, JSLen_HK
dc.contributor.authorLloyd, JMen_HK
dc.contributor.authorLeng, MJen_HK
dc.contributor.authorHeaton, Ten_HK
dc.contributor.authorKendrick, CPen_HK
dc.contributor.authorYongqiang, Zen_HK
dc.date.accessioned2011-09-21T04:15:51Z-
dc.date.available2011-09-21T04:15:51Z-
dc.date.issued2011en_HK
dc.identifier.citationQuaternary Science Reviews, 2011, v. 30 n. 21-22, p. 3076-3087en_HK
dc.identifier.issn0277-3791en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138795-
dc.description.abstractThere is a known bias in C/N, δ 13C and δ 15N values of organic matter (OM) due to pre-analysis acid treatment methods. We report here, for the first time, the results of a pre-analysis acid treatment method comparison of measured C/N, δ 13C and δ 15N values in bulk OM from a sedimentary sequence of samples to illustrate this bias. Here we show that acid treatment significantly reduces the accuracy (between method biases) and precision (within method bias) of C/N, δ 13C and δ 15N values of OM, suggesting a differential response of sample OM between methods and sample horizons, and in some cases inefficient removal of inorganic carbon. We show that different methods can significantly influence environmental interpretation in some of our sample horizons (i.e. interpretation of aquatic vs. terrestrial OM source; C 3 vs. C 4 vegetation). Specifically, there are unpredictable and non-linear differences between methods for C/N values in the range of ∼1-100; δ 13C values in the range of 0.2-6.8‰ and; δ 15N values in the range of 0.3-0.7‰. Importantly, these ranges are mostly much greater than the instrument precision (defined as the standard deviation of replicate analysis of standard reference materials; for this study, ±0.5 for C/N values, ±0.1‰ for δ 13C values and; ±0.1‰ for δ 15N). The accuracy and precision of measured C/N, δ 13C and δ 15N values of bulk OM is not just dependent upon environmental variability, but on acid pre-treatment, residual inorganic carbon and organic matter state and composition. Collectively, this makes the correlation between samples prepared in different ways, including those from down-core reconstructions, highly questionable. © 2011 Elsevier Ltd.en_HK
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/quascireven_HK
dc.relation.ispartofQuaternary Science Reviewsen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Quaternary Science Reviews. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Quaternary Science Reviews, 2011, v. 30 n. 21-22, p. 3076-3087. DOI: 10.1016/j.quascirev.2011.07.003-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectδ13Cen_HK
dc.subjectδ15Nen_HK
dc.subjectC/N ratiosen_HK
dc.subjectEnvironmental interpretationen_HK
dc.subjectOrganic matteren_HK
dc.subjectPalaeoclimateen_HK
dc.subjectPre-analysis acid treatment methodsen_HK
dc.titleEvidence for bias in C/N, δ13C and δ15N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methodsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0277-3791&volume=30&issue=21-22&spage=3076&epage=3087&date=2011&atitle=Evidence+for+bias+in+C/N,+δ13C+and+δ15N+values+of+bulk+organic+matter,+and+on+environmental+interpretation,+from+a+lake+sedimentary+sequence+by+pre-analysis+acid+treatment+methods-
dc.identifier.emailYongqiang, Z:yqzong@hkucc.hku.hken_HK
dc.identifier.authorityYongqiang, Z=rp00846en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.quascirev.2011.07.003en_HK
dc.identifier.scopuseid_2-s2.0-80052930707en_HK
dc.identifier.hkuros195742-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052930707&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue21-22en_HK
dc.identifier.spage3076en_HK
dc.identifier.epage3087en_HK
dc.identifier.isiWOS:000295663500015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBrodie, CR=36917954200en_HK
dc.identifier.scopusauthoridCasford, JSL=6505849496en_HK
dc.identifier.scopusauthoridLloyd, JM=7402365382en_HK
dc.identifier.scopusauthoridLeng, MJ=7006497331en_HK
dc.identifier.scopusauthoridHeaton, T=7006491574en_HK
dc.identifier.scopusauthoridKendrick, CP=35210597000en_HK
dc.identifier.scopusauthoridYongqiang, Z=7005203454en_HK
dc.identifier.citeulike9691597-

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