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Conference Paper: Buyang Huanwu Decoction can promote the proliferation of neural progenitor cell and neurogenesis induced by ischemia stroke via Jak2/stat3-hes1 crosstalk

TitleBuyang Huanwu Decoction can promote the proliferation of neural progenitor cell and neurogenesis induced by ischemia stroke via Jak2/stat3-hes1 crosstalk
Authors
Issue Date2011
PublisherInternational Society for Cerebral Blood Flow & Metabolism (ISCBFM)
Citation
The 25th International Symposium on Cerebral Blood Flow, Metabolism and Function & the 10th International Conference on Quantification of Brain Function with PET, Barcelona, Spain, 25-28 May 2011. How to Cite?
AbstractOBJECTIVES: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for about 200 years. The molecular mechanisms are still unknown. In order to explore the molecular mechanisms of the formula for stroke treatment, we investigated the effects of BHD on modulating cellular growth signals and improving neural stem cells proliferation and differentiation in the experimental rat stroke model by the occlusion of middle cerebral artery (MCAO). METHODS: BHD was orally administrated to the rats for 14and 28 days after MCAO. We detected the proliferation-promoting effects by immunofluorescent staining of thymidine analog 5-bromo-2′-deoxyuridine (BrdU). We also detected the differentiation-promoting effects by immunofluorescence double staining of doublecortin (DCX) and neuron-specific nuclear antigen (NeuN) and astrocyte marker (GFAP). To understand its mechanisms, we investigated the expressions of proteins involved in Jak/stat3 pathway and hes1 which is important signaling molecule to maintain the neural stem cells at the early stage and improve neurogenesis on the later period. RESULTS: The results showed that the formula stimulated the proliferation of the neural stem cells at dentate gyrus of hippocampus in the ischemic brains. BHD could increase the number of the double staining positive cells. We found the formula remarkably up-regulated the expressions of p-Jak, p-stats3, Cylin D1 and hes1 in vitro. CONCLUSION: These result indicated that BHD could improve the neural stem cell proliferation and differentiation, and the underlying mechanism were related to activate Jak/p-Stat3 -hes1 crosstalk.
DescriptionPosters - Cerebral Ischemia: reperfusion: P13
Persistent Identifierhttp://hdl.handle.net/10722/138262

 

DC FieldValueLanguage
dc.contributor.authorChen, Xen_US
dc.contributor.authorShen, Jen_US
dc.date.accessioned2011-08-26T14:43:56Z-
dc.date.available2011-08-26T14:43:56Z-
dc.date.issued2011en_US
dc.identifier.citationThe 25th International Symposium on Cerebral Blood Flow, Metabolism and Function & the 10th International Conference on Quantification of Brain Function with PET, Barcelona, Spain, 25-28 May 2011.en_US
dc.identifier.urihttp://hdl.handle.net/10722/138262-
dc.descriptionPosters - Cerebral Ischemia: reperfusion: P13-
dc.description.abstractOBJECTIVES: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for about 200 years. The molecular mechanisms are still unknown. In order to explore the molecular mechanisms of the formula for stroke treatment, we investigated the effects of BHD on modulating cellular growth signals and improving neural stem cells proliferation and differentiation in the experimental rat stroke model by the occlusion of middle cerebral artery (MCAO). METHODS: BHD was orally administrated to the rats for 14and 28 days after MCAO. We detected the proliferation-promoting effects by immunofluorescent staining of thymidine analog 5-bromo-2′-deoxyuridine (BrdU). We also detected the differentiation-promoting effects by immunofluorescence double staining of doublecortin (DCX) and neuron-specific nuclear antigen (NeuN) and astrocyte marker (GFAP). To understand its mechanisms, we investigated the expressions of proteins involved in Jak/stat3 pathway and hes1 which is important signaling molecule to maintain the neural stem cells at the early stage and improve neurogenesis on the later period. RESULTS: The results showed that the formula stimulated the proliferation of the neural stem cells at dentate gyrus of hippocampus in the ischemic brains. BHD could increase the number of the double staining positive cells. We found the formula remarkably up-regulated the expressions of p-Jak, p-stats3, Cylin D1 and hes1 in vitro. CONCLUSION: These result indicated that BHD could improve the neural stem cell proliferation and differentiation, and the underlying mechanism were related to activate Jak/p-Stat3 -hes1 crosstalk.-
dc.languageengen_US
dc.publisherInternational Society for Cerebral Blood Flow & Metabolism (ISCBFM)-
dc.relation.ispartofInternational Symposium on Cerebral Blood Flow, Metabolism and Function, ISCBFM 2011en_US
dc.relation.ispartofBrain'11-
dc.relation.ispartofInternational Conference on Quantification of Brain Function with PET, BrainPet'11-
dc.titleBuyang Huanwu Decoction can promote the proliferation of neural progenitor cell and neurogenesis induced by ischemia stroke via Jak2/stat3-hes1 crosstalken_US
dc.typeConference_Paperen_US
dc.identifier.emailChen, X: beatingu@hku.hken_US
dc.identifier.emailShen, J: shenjg@hku.hk-
dc.identifier.authorityShen, J=rp00487en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros190939en_US
dc.publisher.placeSpain-

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