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Article: Ginkgo biloba extract (EGb761) inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxia-reoxygenated cardiomyocytes

TitleGinkgo biloba extract (EGb761) inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxia-reoxygenated cardiomyocytes
Authors
Issue Date2011
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/home
Citation
Chinese Medicine, 2011, v. 6 How to Cite?
AbstractBackground: EGb761 is a standard extract from the leaves of Ginkgo biloba (Yinxing) containing ginkgo-flavone glycosides and terpenoid. The flavonoid components of EGb761 scavenge free radicals and protect myocardia from ischemia-reperfusion injury. The present study aims to determine the effects of the active compounds of EGb761 on mitochondria-dependent caspase pathway.Methods: Cardiomyocytes were exposed to 24 hours of hypoxia and four hours of reoxygenation, and pretreated with EGb761, bilobalide and quertcetin. By using immunoblot, immunofluorescent, biochemical and flow cytometry techniques, we compared the effects of EGb761 and its representative constituents including quercetin and bilobalides on regulating mitochondria-dependent caspases signal pathway and apoptotic cell death in the hypoxia-reoxygenated cardiomyocytes.Results: Pretreatment with EGb761 significantly inhibited the release of cytochrome c from mitochondria, the expression of caspase-3, cleavage activities of caspases and attenuated apoptotic cell death. The effects of quercetin on the release of cytochrome c, the cleavage activities of caspases and cell death were similar to those of EGb761 but better than those of bilobalide.Conclusion: The antioxidant constituents of EGb761 such as quercetin contribute to the cardioprotective effects of EGb761 and inhibit the mitochondria-dependent caspase pathway. It is possible that the mitochondria-dependent caspase pathway may be one of the molecular targets of EGb761 against myocardial ischemia-reperfusion injury. © 2011 Shen et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/138133
ISSN
2015 Impact Factor: 1.58
2015 SCImago Journal Rankings: 0.655
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, Jen_HK
dc.contributor.authorLee, Wen_HK
dc.contributor.authorGu, Yen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorFung, PCWen_HK
dc.contributor.authorTong, Len_HK
dc.date.accessioned2011-08-26T14:41:17Z-
dc.date.available2011-08-26T14:41:17Z-
dc.date.issued2011en_HK
dc.identifier.citationChinese Medicine, 2011, v. 6en_HK
dc.identifier.issn1749-8546en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138133-
dc.description.abstractBackground: EGb761 is a standard extract from the leaves of Ginkgo biloba (Yinxing) containing ginkgo-flavone glycosides and terpenoid. The flavonoid components of EGb761 scavenge free radicals and protect myocardia from ischemia-reperfusion injury. The present study aims to determine the effects of the active compounds of EGb761 on mitochondria-dependent caspase pathway.Methods: Cardiomyocytes were exposed to 24 hours of hypoxia and four hours of reoxygenation, and pretreated with EGb761, bilobalide and quertcetin. By using immunoblot, immunofluorescent, biochemical and flow cytometry techniques, we compared the effects of EGb761 and its representative constituents including quercetin and bilobalides on regulating mitochondria-dependent caspases signal pathway and apoptotic cell death in the hypoxia-reoxygenated cardiomyocytes.Results: Pretreatment with EGb761 significantly inhibited the release of cytochrome c from mitochondria, the expression of caspase-3, cleavage activities of caspases and attenuated apoptotic cell death. The effects of quercetin on the release of cytochrome c, the cleavage activities of caspases and cell death were similar to those of EGb761 but better than those of bilobalide.Conclusion: The antioxidant constituents of EGb761 such as quercetin contribute to the cardioprotective effects of EGb761 and inhibit the mitochondria-dependent caspase pathway. It is possible that the mitochondria-dependent caspase pathway may be one of the molecular targets of EGb761 against myocardial ischemia-reperfusion injury. © 2011 Shen et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/homeen_HK
dc.relation.ispartofChinese Medicineen_HK
dc.rightsChinese Medicine. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleGinkgo biloba extract (EGb761) inhibits mitochondria-dependent caspase pathway and prevents apoptosis in hypoxia-reoxygenated cardiomyocytesen_HK
dc.typeArticleen_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1749-8546-6-8en_HK
dc.identifier.pmid21345217-
dc.identifier.pmcidPMC3053309-
dc.identifier.scopuseid_2-s2.0-79951854650en_HK
dc.identifier.hkuros190885en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79951854650&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.isiWOS:000208722100008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.scopusauthoridLee, W=12788473400en_HK
dc.identifier.scopusauthoridGu, Y=37014467100en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridFung, PCW=7101613315en_HK
dc.identifier.scopusauthoridTong, L=7201891650en_HK

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