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Article: Caveolin-1 inhibits oligodendroglial differentiation of neural stem/progenitor cells through modulating β-catenin expression

TitleCaveolin-1 inhibits oligodendroglial differentiation of neural stem/progenitor cells through modulating β-catenin expression
Authors
Keywordsβ-Catenin
Caveolin-1
Neural progenitor cells
Oligodendrocytes
Issue Date2011
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neuint
Citation
Neurochemistry International, 2011, v. 59 n. 2, p. 114-121 How to Cite?
AbstractIn the present study, we aim to elucidate the role of caveolin-1 (Cav-1) in modulating oligodendroglial differentiation of neural progenitor cells (NPCs) in vivo and in vitro. For in vivo experiments, we investigated oligodendroglial differentiation by detecting the expressions of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and β-catenin in the brains of wild type mice and Cav-1 knockout mice. Cav-1 knockout mice revealed more oligodendroglial differentiation, but lower levels of β-catenin expression than wild type mice. For in vitro experiments, we observed the potential roles of Cav-1 in modulating β-catenin expression and oligodendroglial differentiation in isolated cultured NPCs by manipulating Cav-1 expression with Cav-1 scaffolding domain peptide and Cav-1 RNA silencing approach. In the differentiating NPCs, Cav-1 scaffolding domain peptide markedly inhibited oligodendroglial formation, but up-regulated the expression of β-catenin. In contrast, the knockdown of Cav-1 promoted oligodendroglial differentiation of NPCs, but down-regulated the expression of β-catenin. Taken together, these results directly prove that caveolin-1 can inhibit oligodendroglial differentiation of NPCs through modulating β-catenin expression. © 2011 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/138130
ISSN
2015 Impact Factor: 3.385
2015 SCImago Journal Rankings: 1.345
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong RGC General Research Fund777610M
774808M
University of Hong Kong200807176043
Funding Information:

We thank Dr. Shu-Ping Fu and Ms. Xing-Miao Chen for their technical assistances for Caveolin-1 knockout mice genotyping. This work was supported by Hong Kong RGC General Research Fund (GRF Nos. 777610M and 774808M, J.G.S.), Small Project Funding of the University of Hong Kong (200807176043, J.G.S.) and Dr. Wong BL family donation (J.G.S.).

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_HK
dc.contributor.authorLau, WMen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorShen, Jen_HK
dc.date.accessioned2011-08-26T14:41:14Z-
dc.date.available2011-08-26T14:41:14Z-
dc.date.issued2011en_HK
dc.identifier.citationNeurochemistry International, 2011, v. 59 n. 2, p. 114-121en_HK
dc.identifier.issn0197-0186en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138130-
dc.description.abstractIn the present study, we aim to elucidate the role of caveolin-1 (Cav-1) in modulating oligodendroglial differentiation of neural progenitor cells (NPCs) in vivo and in vitro. For in vivo experiments, we investigated oligodendroglial differentiation by detecting the expressions of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) and β-catenin in the brains of wild type mice and Cav-1 knockout mice. Cav-1 knockout mice revealed more oligodendroglial differentiation, but lower levels of β-catenin expression than wild type mice. For in vitro experiments, we observed the potential roles of Cav-1 in modulating β-catenin expression and oligodendroglial differentiation in isolated cultured NPCs by manipulating Cav-1 expression with Cav-1 scaffolding domain peptide and Cav-1 RNA silencing approach. In the differentiating NPCs, Cav-1 scaffolding domain peptide markedly inhibited oligodendroglial formation, but up-regulated the expression of β-catenin. In contrast, the knockdown of Cav-1 promoted oligodendroglial differentiation of NPCs, but down-regulated the expression of β-catenin. Taken together, these results directly prove that caveolin-1 can inhibit oligodendroglial differentiation of NPCs through modulating β-catenin expression. © 2011 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neuinten_HK
dc.relation.ispartofNeurochemistry Internationalen_HK
dc.subjectβ-Cateninen_HK
dc.subjectCaveolin-1en_HK
dc.subjectNeural progenitor cellsen_HK
dc.subjectOligodendrocytesen_HK
dc.titleCaveolin-1 inhibits oligodendroglial differentiation of neural stem/progenitor cells through modulating β-catenin expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0197-0186&volume=59&issue=2&spage=114&epage=121&date=2011&atitle=Caveolin-1+inhibits+oligodendroglial+differentiation+of+neural+stem/progenitor+cells+through+modulating+β-catenin+expression-
dc.identifier.emailSo, KF: hrmaskf@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.neuint.2011.05.019en_HK
dc.identifier.pmid21693146-
dc.identifier.scopuseid_2-s2.0-79960363199en_HK
dc.identifier.hkuros190866en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960363199&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue2en_HK
dc.identifier.spage114en_HK
dc.identifier.epage121en_HK
dc.identifier.isiWOS:000294091600005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, Y=36671636100en_HK
dc.identifier.scopusauthoridLau, WM=16239172000en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.citeulike9494309-

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