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Article: Spatholobus suberectus inhibits cancer cell growth by inducing apoptosis and arresting cell cycle at G2/M checkpoint

TitleSpatholobus suberectus inhibits cancer cell growth by inducing apoptosis and arresting cell cycle at G2/M checkpoint
Authors
KeywordsApoptosis
Breast and colon cancer
Cell cycle
ROS
Spatholobus suberectus
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2011, v. 133 n. 2, p. 751-758 How to Cite?
AbstractAim of the study: Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods: The effect of SS on cell proliferation was studied by cell growth assay and flow cytometry on breast cancer cell lines MCF-7 and colon cancer cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation assay and mitochondrial membrane potential assay. Expression of proteins associated with cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results: Cell growth assay showed that SS effectively inhibits tumor cell growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions: Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor growth via induction of apoptosis and arrest of the cell cycle at G2/M phase. © 2010 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/138123
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, ZYen_HK
dc.contributor.authorWang, DMen_HK
dc.contributor.authorLoo, TYen_HK
dc.contributor.authorCheng, Yen_HK
dc.contributor.authorChen, LLen_HK
dc.contributor.authorShen, JGen_HK
dc.contributor.authorYang, DPen_HK
dc.contributor.authorChow, LWCen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorChen, JPen_HK
dc.date.accessioned2011-08-26T14:41:07Z-
dc.date.available2011-08-26T14:41:07Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2011, v. 133 n. 2, p. 751-758en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138123-
dc.description.abstractAim of the study: Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated. Materials and methods: The effect of SS on cell proliferation was studied by cell growth assay and flow cytometry on breast cancer cell lines MCF-7 and colon cancer cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation assay and mitochondrial membrane potential assay. Expression of proteins associated with cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts. Results: Cell growth assay showed that SS effectively inhibits tumor cell growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals. Conclusions: Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor growth via induction of apoptosis and arrest of the cell cycle at G2/M phase. © 2010 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectApoptosisen_HK
dc.subjectBreast and colon canceren_HK
dc.subjectCell cycleen_HK
dc.subjectROSen_HK
dc.subjectSpatholobus suberectusen_HK
dc.subject.meshAntineoplastic Agents, Phytogenic - pharmacology-
dc.subject.meshDrugs, Chinese Herbal - pharmacology-
dc.subject.meshFabaceae - chemistry-
dc.subject.meshNeoplasms - drug therapy - metabolism - pathology-
dc.subject.meshPhytotherapy-
dc.titleSpatholobus suberectus inhibits cancer cell growth by inducing apoptosis and arresting cell cycle at G2/M checkpointen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=133&issue=2&spage=751&epage=758&date=2010&atitle=Spatholobus+sub+erectus+inhibits+cancer+cell+growth+by+inducing+apoptosis+and+arresting+cell+cycle+at+G2/M+checkpointen_US
dc.identifier.emailShen, JG: shenjg@hku.hken_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.emailChen, JP: abchen@hku.hken_HK
dc.identifier.authorityShen, JG=rp00487en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.authorityChen, JP=rp01316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2010.11.004en_HK
dc.identifier.pmid21073941-
dc.identifier.scopuseid_2-s2.0-78651381614en_HK
dc.identifier.hkuros189753en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78651381614&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume133en_HK
dc.identifier.issue2en_HK
dc.identifier.spage751en_HK
dc.identifier.epage758en_HK
dc.identifier.isiWOS:000290004100064-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridWang, ZY=7410054221en_HK
dc.identifier.scopusauthoridWang, DM=36955783900en_HK
dc.identifier.scopusauthoridLoo, TY=7006008583en_HK
dc.identifier.scopusauthoridCheng, Y=35795014500en_HK
dc.identifier.scopusauthoridChen, LL=39560982600en_HK
dc.identifier.scopusauthoridShen, JG=7404929947en_HK
dc.identifier.scopusauthoridYang, DP=22959396400en_HK
dc.identifier.scopusauthoridChow, LWC=7202532995en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridChen, JP=22733695400en_HK
dc.identifier.citeulike8336443-

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