File Download
  • No File Attached
 
 
Supplementary

Article: Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus
  • Basic View
  • Metadata View
  • XML View
TitleTotal flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus
不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響
 
AuthorsCheng, Y
Wang, ZY
Wang, DM
Liu, HW
Chen, J
 
KeywordsSpatholobus suberectus (雞血藤)
Anti-tumor (抗腫瘤)
Total flavonoids (總黃酮)
Formononetin (芒柄花素)
Cytotoxic activity (細胞毒性)
 
Issue Date2011
 
Publisher中國中醫硏究院中藥硏究所, 中國中西醫結合學會.
 
Citation中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145
Chinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145 [How to Cite?]
 
AbstractObjective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。
 
DescriptionThe article can be viewed at http://www.syfjxzz.com/ch/reader/view_abstract.aspx?flag=1&file_no=20110941&journal_id=zgsyfjxzz
The article can also be viewed at http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZSFX201109043.htm
 
ISSN1005-9903
 
DC FieldValue
dc.contributor.authorCheng, Y
 
dc.contributor.authorWang, ZY
 
dc.contributor.authorWang, DM
 
dc.contributor.authorLiu, HW
 
dc.contributor.authorChen, J
 
dc.date.accessioned2011-08-26T14:41:05Z
 
dc.date.available2011-08-26T14:41:05Z
 
dc.date.issued2011
 
dc.description.abstractObjective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。
 
dc.descriptionThe article can be viewed at http://www.syfjxzz.com/ch/reader/view_abstract.aspx?flag=1&file_no=20110941&journal_id=zgsyfjxzz
 
dc.descriptionThe article can also be viewed at http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZSFX201109043.htm
 
dc.identifier.citation中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145
 
dc.identifier.citationChinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145 [How to Cite?]
 
dc.identifier.epage145
 
dc.identifier.hkuros189752
 
dc.identifier.issn1005-9903
 
dc.identifier.issue9
 
dc.identifier.spage142
 
dc.identifier.urihttp://hdl.handle.net/10722/138122
 
dc.identifier.volume17
 
dc.languagechi
 
dc.publisher中國中醫硏究院中藥硏究所, 中國中西醫結合學會.
 
dc.publisher.placeChina
 
dc.relation.ispartof中國實驗方劑學雜誌
 
dc.relation.ispartofChinese Journal of Experimental Traditional Medical Formulae
 
dc.subjectSpatholobus suberectus (雞血藤)
 
dc.subjectAnti-tumor (抗腫瘤)
 
dc.subjectTotal flavonoids (總黃酮)
 
dc.subjectFormononetin (芒柄花素)
 
dc.subjectCytotoxic activity (細胞毒性)
 
dc.titleTotal flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus
 
dc.title不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Cheng, Y</contributor.author>
<contributor.author>Wang, ZY</contributor.author>
<contributor.author>Wang, DM</contributor.author>
<contributor.author>Liu, HW</contributor.author>
<contributor.author>Chen, J</contributor.author>
<date.accessioned>2011-08-26T14:41:05Z</date.accessioned>
<date.available>2011-08-26T14:41:05Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>&#20013;&#22283;&#23526;&#39511;&#26041;&#21137;&#23416;&#38620;&#35468;, 2011, v. 17 n. 9, p. 142-145</identifier.citation>
<identifier.citation>Chinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145</identifier.citation>
<identifier.issn>1005-9903</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/138122</identifier.uri>
<description>The article can be viewed at http://www.syfjxzz.com/ch/reader/view_abstract.aspx?flag=1&amp;file_no=20110941&amp;journal_id=zgsyfjxzz</description>
<description>The article can also be viewed at http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZSFX201109043.htm</description>
<description.abstract>Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell.
&#30446;&#30340;:&#25506;&#35342;&#19981;&#21516;&#28342;&#21137;&#23565;&#38622;&#34880;&#34276;&#25552;&#21462;&#29289;&#20013;&#30340;&#32317;&#40643;&#37230;&#21547;&#37327;&#21644;&#25239;&#33131;&#30244;&#27963;&#24615;&#30340;&#24433;&#38911;&#12290;&#26041;&#27861;:&#20998;&#21029;&#29992;&#19981;&#21516;&#28611;&#24230;&#30340;&#20057;&#37255;&#27700;&#28342;&#28082;&#12289;&#19993;&#37230;&#27700;&#28342;&#28082;&#20316;&#28858;&#25552;&#21462;&#28342;&#21137;,&#21033;&#29992;&#22238;&#27969;&#27861;&#12289;&#36229;&#32882;&#25552;&#21462;&#27861;&#21046;&#20633;&#38622;&#34880;&#34276;&#25552;&#21462;&#29289;;&#37319;&#29992;&#22235;&#30002;&#22522;&#22139;&#21777;&#34253;(MTT)&#27861;&#28204;&#23450;&#25552;&#21462;&#29289;&#23565;&#32080;&#33144;&#30284;HT-29&#32048;&#32990;&#22686;&#27542;&#30340;&#25233;&#21046;&#27963;&#24615;;&#37319;&#29992;&#20998;&#20809;&#20809;&#24230;&#27861;&#28204;&#23450;&#25552;&#21462;&#29289;&#20013;&#32317;&#40643;&#37230;&#21547;&#37327;;&#37319;&#29992;HPLC&#27861;&#28204;&#23450;&#25552;&#21462;&#29289;&#20013;&#33426;&#26564;&#33457;&#32032;&#21547;&#37327;&#12290;&#32080;&#26524;:60%&#20057;&#37255;&#22238;&#27969;60 min&#25152;&#24471;&#25552;&#21462;&#29289;&#32317;&#40643;&#37230;&#21547;&#37327;&#26368;&#39640;&#24182;&#19988;&#23565;HT-29&#32048;&#32990;&#22686;&#27542;&#30340;&#25233;&#21046;&#27963;&#24615;&#26368;&#24375;&#12290;&#32080;&#35542;:&#38622;&#34880;&#34276;&#25239;&#33131;&#30244;&#27963;&#24615;&#25552;&#21462;&#29289;&#30340;&#21046;&#20633;&#20197;60%&#20057;&#37255;&#22238;&#27969;&#25552;&#21462;&#28858;&#20339;&#12290;</description.abstract>
<language>chi</language>
<publisher>&#20013;&#22283;&#20013;&#37291;&#30799;&#31350;&#38498;&#20013;&#34277;&#30799;&#31350;&#25152;, &#20013;&#22283;&#20013;&#35199;&#37291;&#32080;&#21512;&#23416;&#26371;.</publisher>
<relation.ispartof>&#20013;&#22283;&#23526;&#39511;&#26041;&#21137;&#23416;&#38620;&#35468;</relation.ispartof>
<relation.ispartof>Chinese Journal of Experimental Traditional Medical Formulae</relation.ispartof>
<subject>Spatholobus suberectus (&#38622;&#34880;&#34276;)</subject>
<subject>Anti-tumor (&#25239;&#33131;&#30244;)</subject>
<subject>Total flavonoids (&#32317;&#40643;&#37230;)</subject>
<subject>Formononetin (&#33426;&#26564;&#33457;&#32032;)</subject>
<subject>Cytotoxic activity (&#32048;&#32990;&#27602;&#24615;)</subject>
<title>Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus</title>
<title>&#19981;&#21516;&#28342;&#21137;&#23565;&#38622;&#34880;&#34276;&#25552;&#21462;&#29289;&#32317;&#40643;&#37230;&#21547;&#37327;&#21450;&#25239;&#33131;&#30244;&#27963;&#24615;&#30340;&#24433;&#38911;</title>
<type>Article</type>
<identifier.hkuros>189752</identifier.hkuros>
<identifier.volume>17</identifier.volume>
<identifier.issue>9</identifier.issue>
<identifier.spage>142</identifier.spage>
<identifier.epage>145</identifier.epage>
<publisher.place>China</publisher.place>
</item>