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Article: Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus

TitleTotal flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus
不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響
Authors
KeywordsSpatholobus suberectus (雞血藤)
Anti-tumor (抗腫瘤)
Total flavonoids (總黃酮)
Formononetin (芒柄花素)
Cytotoxic activity (細胞毒性)
Issue Date2011
Publisher中國中醫硏究院中藥硏究所, 中國中西醫結合學會.
Citation
中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145 How to Cite?
Chinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145 How to Cite?
AbstractObjective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。
Persistent Identifierhttp://hdl.handle.net/10722/138122
ISSN

 

DC FieldValueLanguage
dc.contributor.authorCheng, Yen_US
dc.contributor.authorWang, ZYen_US
dc.contributor.authorWang, DMen_US
dc.contributor.authorLiu, HWen_US
dc.contributor.authorChen, Jen_US
dc.date.accessioned2011-08-26T14:41:05Z-
dc.date.available2011-08-26T14:41:05Z-
dc.date.issued2011en_US
dc.identifier.citation中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145zh_HK
dc.identifier.citationChinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145en_US
dc.identifier.issn1005-9903-
dc.identifier.urihttp://hdl.handle.net/10722/138122-
dc.description.abstractObjective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。zh_HK
dc.languagechien_US
dc.publisher中國中醫硏究院中藥硏究所, 中國中西醫結合學會.zh_HK
dc.relation.ispartof中國實驗方劑學雜誌zh_HK
dc.relation.ispartofChinese Journal of Experimental Traditional Medical Formulaeen_US
dc.subjectSpatholobus suberectus (雞血藤)zh_HK
dc.subjectAnti-tumor (抗腫瘤)zh_HK
dc.subjectTotal flavonoids (總黃酮)zh_HK
dc.subjectFormononetin (芒柄花素)zh_HK
dc.subjectCytotoxic activity (細胞毒性)zh_HK
dc.titleTotal flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectusen_US
dc.title不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響zh_HK
dc.typeArticleen_US
dc.identifier.emailChen, J: abchen@hku.hken_US
dc.identifier.authorityChen, J=rp01316en_US
dc.identifier.hkuros189752en_US
dc.identifier.volume17en_US
dc.identifier.issue9en_US
dc.identifier.spage142en_US
dc.identifier.epage145en_US
dc.publisher.placeChina-

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