Article: Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus

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Title	Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus 不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響
Authors	Cheng, Y Wang, ZY Wang, DM Liu, HW Chen, J
Keywords	Spatholobus suberectus (雞血藤) Anti-tumor (抗腫瘤) Total flavonoids (總黃酮) Formononetin (芒柄花素) Cytotoxic activity (細胞毒性)
Issue Date	2011
Publisher	中國中醫硏究院中藥硏究所, 中國中西醫結合學會.
Citation	中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145 Chinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145 [How to Cite?]
Abstract	Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。
Description	The article can be viewed at http://www.syfjxzz.com/ch/reader/view_abstract.aspx?flag=1&file_no=20110941&journal_id=zgsyfjxzz The article can also be viewed at http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZSFX201109043.htm
ISSN	1005-9903

DC Field	Value
dc.contributor.author	Cheng, Y
dc.contributor.author	Wang, ZY
dc.contributor.author	Wang, DM
dc.contributor.author	Liu, HW
dc.contributor.author	Chen, J
dc.date.accessioned	2011-08-26T14:41:05Z
dc.date.available	2011-08-26T14:41:05Z
dc.date.issued	2011
dc.description.abstract	Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent, different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC, respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent, refluxing as extraction method, obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell. 目的:探討不同溶劑對雞血藤提取物中的總黃酮含量和抗腫瘤活性的影響。方法:分別用不同濃度的乙醇水溶液、丙酮水溶液作為提取溶劑,利用回流法、超聲提取法制備雞血藤提取物;采用四甲基噻唑藍(MTT)法測定提取物對結腸癌HT-29細胞增殖的抑制活性;采用分光光度法測定提取物中總黃酮含量;采用HPLC法測定提取物中芒柄花素含量。結果:60%乙醇回流60 min所得提取物總黃酮含量最高并且對HT-29細胞增殖的抑制活性最強。結論:雞血藤抗腫瘤活性提取物的制備以60%乙醇回流提取為佳。
dc.description	The article can be viewed at http://www.syfjxzz.com/ch/reader/view_abstract.aspx?flag=1&file_no=20110941&journal_id=zgsyfjxzz
dc.description	The article can also be viewed at http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZSFX201109043.htm
dc.identifier.citation	中國實驗方劑學雜誌, 2011, v. 17 n. 9, p. 142-145
dc.identifier.citation	Chinese Journal of Experimental Traditional Medical Formulae, 2011, v. 17 n. 9, p. 142-145 [How to Cite?]
dc.identifier.epage	145
dc.identifier.hkuros	189752
dc.identifier.issn	1005-9903
dc.identifier.issue	9
dc.identifier.spage	142
dc.identifier.uri	http://hdl.handle.net/10722/138122
dc.identifier.volume	17
dc.language	chi
dc.publisher	中國中醫硏究院中藥硏究所, 中國中西醫結合學會.
dc.publisher.place	China
dc.relation.ispartof	中國實驗方劑學雜誌
dc.relation.ispartof	Chinese Journal of Experimental Traditional Medical Formulae
dc.subject	Spatholobus suberectus (雞血藤)
dc.subject	Anti-tumor (抗腫瘤)
dc.subject	Total flavonoids (總黃酮)
dc.subject	Formononetin (芒柄花素)
dc.subject	Cytotoxic activity (細胞毒性)
dc.title	Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus
dc.title	不同溶劑對雞血藤提取物總黃酮含量及抗腫瘤活性的影響
dc.type	Article

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Article: Total flavonoids and its anti-tumor activities by different solvent extract from Spatholobus suberectus

The HKU Scholars Hub has contact details for these author(s).