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Article: Expression of Itch in Sertoli cells is controlled via the interaction of E2F1/DP1 complex with E2F and GATA motifs

TitleExpression of Itch in Sertoli cells is controlled via the interaction of E2F1/DP1 complex with E2F and GATA motifs
Authors
KeywordsItch
E3 ligase
Sertoli cells
Gene transcription
E2F/DP complex
Issue Date2011
PublisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/spermatogenesis/
Citation
Spermatogenesis, 2011, v. 1 n. 2, p. 152-158 How to Cite?
AbstractItch, an ubiquitin E3 ligase, has been implicated in the regulation of the permeability of tight junction (TJ) barriers in Sertoli cells. It is involved in cAMP-mediated TJ disruption by targeting occludin for proteasomal degradation in the testis. However, the molecular mechanisms governing its transcription remain enigmatic. By the transient transfection of Itch promoter luciferase construct in TM4 cells, we showed that the minimal Itch promoter was located between nucleotides -151 and -1 (relative to the translation start site). One E2F motif and two each of GATA and Nkx motifs were identified within the core promoter region. Mutation and overexpression analyses have shown that the E2F and GATA-a motifs are involved in Itch gene transcription, but play different roles. The E2F motif is the crucial cis-acting element that drives the basal gene transcription, while the GATA-a motif functionally co-operates with E2F motif. By electromobility shift assays, we confirmed that E2F1 and DP1 form heterodimers and binds to E2F and GATA-a motifs. Taken together, the GATA-a motif assists/strengthens the binding of E2F1/DP1 complex to the E2F motif, resulting in efficient looping of promoter region of Itch gene for transcription.
Persistent Identifierhttp://hdl.handle.net/10722/138076
ISSN
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorLi, WMMen_US
dc.contributor.authorLee, WWMen_US
dc.contributor.authorLui, WYen_US
dc.date.accessioned2011-08-26T14:39:41Z-
dc.date.available2011-08-26T14:39:41Z-
dc.date.issued2011en_US
dc.identifier.citationSpermatogenesis, 2011, v. 1 n. 2, p. 152-158en_US
dc.identifier.issn2156-5554-
dc.identifier.urihttp://hdl.handle.net/10722/138076-
dc.description.abstractItch, an ubiquitin E3 ligase, has been implicated in the regulation of the permeability of tight junction (TJ) barriers in Sertoli cells. It is involved in cAMP-mediated TJ disruption by targeting occludin for proteasomal degradation in the testis. However, the molecular mechanisms governing its transcription remain enigmatic. By the transient transfection of Itch promoter luciferase construct in TM4 cells, we showed that the minimal Itch promoter was located between nucleotides -151 and -1 (relative to the translation start site). One E2F motif and two each of GATA and Nkx motifs were identified within the core promoter region. Mutation and overexpression analyses have shown that the E2F and GATA-a motifs are involved in Itch gene transcription, but play different roles. The E2F motif is the crucial cis-acting element that drives the basal gene transcription, while the GATA-a motif functionally co-operates with E2F motif. By electromobility shift assays, we confirmed that E2F1 and DP1 form heterodimers and binds to E2F and GATA-a motifs. Taken together, the GATA-a motif assists/strengthens the binding of E2F1/DP1 complex to the E2F motif, resulting in efficient looping of promoter region of Itch gene for transcription.-
dc.languageengen_US
dc.publisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/spermatogenesis/-
dc.relation.ispartofSpermatogenesisen_US
dc.subjectItch-
dc.subjectE3 ligase-
dc.subjectSertoli cells-
dc.subjectGene transcription-
dc.subjectE2F/DP complex-
dc.titleExpression of Itch in Sertoli cells is controlled via the interaction of E2F1/DP1 complex with E2F and GATA motifsen_US
dc.typeArticleen_US
dc.identifier.emailLee, WWM: hrszlwm@hku.hken_US
dc.identifier.emailLui, WY: wylui@hku.hken_US
dc.identifier.authorityLee, WWM=rp00728en_US
dc.identifier.authorityLui, WY=rp00756en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4161/spmg.1.2.16957-
dc.identifier.pmid22319664-
dc.identifier.pmcidPMC3271658-
dc.identifier.hkuros191894en_US
dc.identifier.volume1en_US
dc.identifier.issue2-
dc.identifier.spage152en_US
dc.identifier.epage158en_US
dc.publisher.placeUnited States-

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