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Conference Paper: Wnt2 secreted by tumor fibroblasts promotes tumor growth and cell motility in esophageal carcinoma

TitleWnt2 secreted by tumor fibroblasts promotes tumor growth and cell motility in esophageal carcinoma
Authors
KeywordsMedical sciences
Oncology
Issue Date2011
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
The 102nd Annual Meeting of the American Association for Cancer Research (AACR 2011), Orlando, FL., 2-6 April 2011. In In Cancer Research, 2011, v. 71 n. 8 suppl. 1, abstract no. 3109 How to Cite?
AbstractInteraction between neoplastic and stromal cells plays an important role in the tumor progression. Recently, we found that Wnt2 was frequently overexpressed in fibroblasts isolated from tumor tissue (TFs) compared to fibroblasts from non-tumor tissue (NFs) in esophageal squamous cell carcinoma (ESCC). In the present study, we studied the effect of TF-secreted Wnt2 in ESCC development via the tumor-stroma interaction. Quantitative real-time PCR found that Wnt2 expression was about 4 folds higher in the TFs than the NFs. Interestingly, no visible protein expression of WNT2 was detected in all tested ESCC cancer cell lines and clinical ESCC samples. Immunohistochemistry study showed that WNT2(+) cells was were mainly detected in the boundary between stroma and tumor tissue or scattered within tumor tissue. Furthermore, Wnt2-positive ESCC (defined as 5 or more Wnt2(+) cells observed in 200× microscopy field) was detected in 22/51 cases, which was significantly associated with the lymph node metastases (P=0.001), advanced TNM stage (P=0.001) and disease-specific survival (P<0.0001). To further investigate the effect of secreted Wnt2 on ESCC cells, an in vitro Wnt2 secreting system was established using Chinese hamster ovary (CHO) cells and conditioned medium was collected for functional study. Functional assays demonstrated that secreted Wnt2 could promote cell growth and cell migration. Molecular study found that the cell growth promoting effect of Wnt2 was via activating Wnt/β-catenin signaling pathway and subsequently upregulating cyclin D1 and c-myc expression. Wnt2 could also induce epithelial-mesenchymal transition (EMT) and promote cell motility. In conclusion, our data demonstrate that TF-secreted Wnt2 can promote cell growth through activating the canonical Wnt/β-catenin signaling pathway and increase cell motility by inducing EMT in esophageal cancer cells. Further characterization of the effect of Wnt2 secreted by TF on cancer development and progression will reveal the molecular mechanisms of how TF provide cancer cells a suitable microenvironment and find new therapeutic targets for cancer treatment.
DescriptionPoster Session 11 - Tumor Microenvironment Interactions: abstract no. 3109
This journal suppl. entitled: Proceedings: AACR 102nd Annual Meeting 2011
Persistent Identifierhttp://hdl.handle.net/10722/137867
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_US
dc.contributor.authorFu, Len_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorDong, Sen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorChen, Jen_US
dc.date.accessioned2011-08-26T14:35:43Z-
dc.date.available2011-08-26T14:35:43Z-
dc.date.issued2011en_US
dc.identifier.citationThe 102nd Annual Meeting of the American Association for Cancer Research (AACR 2011), Orlando, FL., 2-6 April 2011. In In Cancer Research, 2011, v. 71 n. 8 suppl. 1, abstract no. 3109en_US
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/137867-
dc.descriptionPoster Session 11 - Tumor Microenvironment Interactions: abstract no. 3109-
dc.descriptionThis journal suppl. entitled: Proceedings: AACR 102nd Annual Meeting 2011-
dc.description.abstractInteraction between neoplastic and stromal cells plays an important role in the tumor progression. Recently, we found that Wnt2 was frequently overexpressed in fibroblasts isolated from tumor tissue (TFs) compared to fibroblasts from non-tumor tissue (NFs) in esophageal squamous cell carcinoma (ESCC). In the present study, we studied the effect of TF-secreted Wnt2 in ESCC development via the tumor-stroma interaction. Quantitative real-time PCR found that Wnt2 expression was about 4 folds higher in the TFs than the NFs. Interestingly, no visible protein expression of WNT2 was detected in all tested ESCC cancer cell lines and clinical ESCC samples. Immunohistochemistry study showed that WNT2(+) cells was were mainly detected in the boundary between stroma and tumor tissue or scattered within tumor tissue. Furthermore, Wnt2-positive ESCC (defined as 5 or more Wnt2(+) cells observed in 200× microscopy field) was detected in 22/51 cases, which was significantly associated with the lymph node metastases (P=0.001), advanced TNM stage (P=0.001) and disease-specific survival (P<0.0001). To further investigate the effect of secreted Wnt2 on ESCC cells, an in vitro Wnt2 secreting system was established using Chinese hamster ovary (CHO) cells and conditioned medium was collected for functional study. Functional assays demonstrated that secreted Wnt2 could promote cell growth and cell migration. Molecular study found that the cell growth promoting effect of Wnt2 was via activating Wnt/β-catenin signaling pathway and subsequently upregulating cyclin D1 and c-myc expression. Wnt2 could also induce epithelial-mesenchymal transition (EMT) and promote cell motility. In conclusion, our data demonstrate that TF-secreted Wnt2 can promote cell growth through activating the canonical Wnt/β-catenin signaling pathway and increase cell motility by inducing EMT in esophageal cancer cells. Further characterization of the effect of Wnt2 secreted by TF on cancer development and progression will reveal the molecular mechanisms of how TF provide cancer cells a suitable microenvironment and find new therapeutic targets for cancer treatment.-
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/-
dc.relation.ispartofCancer Researchen_US
dc.subjectMedical sciences-
dc.subjectOncology-
dc.titleWnt2 secreted by tumor fibroblasts promotes tumor growth and cell motility in esophageal carcinomaen_US
dc.typeConference_Paperen_US
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_US
dc.identifier.emailFu, L: gracefu@graduate.hku.hken_US
dc.identifier.emailDong, S: dongss@hku.hken_US
dc.identifier.authorityGuan, XY=rp00454en_US
dc.identifier.authorityFu, L=rp01435en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/1538-7445.AM2011-3109-
dc.identifier.hkuros190591en_US
dc.identifier.volume71-
dc.identifier.issue8 suppl. 1-
dc.publisher.placeUnited States-

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