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Conference Paper: Low TH17 expression associaton with chronic hepatitis B viral infection by means of reducing viral specific IFNγ releasing in a HBV infection model of IL-17 KO mice

TitleLow TH17 expression associaton with chronic hepatitis B viral infection by means of reducing viral specific IFNγ releasing in a HBV infection model of IL-17 KO mice
Authors
KeywordsIL-17
HBV infection
Animal model
Issue Date2011
PublisherAkademiai Kiado Rt.. The Journal's web site is located at http://akkrt.hu/73/journals/products/medicine/european_journal_of_microbiology_and_immunology
Citation
The 1st European Conference of Microbiology and Immunology (EUCMI2011), Budapest, Hungary, 12-14 May 2011. In European Journal of Microbiology and Immunology, 2011, v. 1 n. 2, p. 99-100 How to Cite?
AbstractBACKGROUND AND AIM: Chronic hepatitis B infection (CHB) is associated with T-cell dependent viral specific immune response. As IL-17 may constitute both early initiator of T-cell inflammatory response and essential element of cytokine network that bridges the immune system to hematopoiesis, some effects on HBV were explored in both patients and IL-17 KO mice. METHODS: Forty-two CHB patients were divided into two groups according to their HBV markers, which are Group A: HBeAg+ with normal ALT and Group B: HbsAg–, anti-HBs+. IL-17A expression level was compared after incubating the peripheral blood mononuclear cells (PBMC) with HBV antigen. Viral specific IFNγ releasing cells and TH17 phenotype were analyzed by ELISpot and FACS respectively. To explore the correlation between Th17 and anti-viral immunity, HBV mice model was created in IL-17 KO mice by hydrodynamic injection (HDI) through the tail vein with PAAV-HBV plasmid. Results: After incubation with HBcAg, IL-17 mRNA expression, Th17 and IFNγ frequencies increased significantly in Group B than in Group A (P<0.01 to 0.001). Th17/CD45RO frequency was significantly higher in Group B than in Group A. Both Th17 and IFNγ-releasing cells mostly come from memory activated T cells. In Group A, IFNγ releasing cells increased significantly and dose-dependently when the PBMCs were exposed to IL-17. IL-17 itself did not enhance PBMCs’ ability to kill AD38 and HepG2 cell lines. It has neither inhibition to HBV replication in AD38 no cytotoxicity to HepG2 growth in vitro. Three weeks after HBV-plasmid injection, HBsAg was completely clearance in wild type C57, while it was until the 3rd month after HBV HDI that all 19 IL-17 KO mice became HBsAg negative. Viral specific IFN releasing cells were much lower in IL-17 KO mice than in wild type mice during the first two weeks after HBV HDI. Anti-HBs antibody was 1000 times lower in IL-17 KO mice than in wild type mice. CONCLUSION: Anti-HBV immunity depends on Th17 as well as IL-17 expression in both CHB patients and HBV mice model at the early stage of infection. Low expression in IL-17 might be one of important factors associated with chronic HBV infection.
DescriptionOral Presentation - Session 2
Persistent Identifierhttp://hdl.handle.net/10722/137795
ISSN

 

DC FieldValueLanguage
dc.contributor.authorZhang, Hen_US
dc.contributor.authorLin, Yen_US
dc.contributor.authorZhang, Ken_US
dc.contributor.authorLiao, Wen_US
dc.contributor.authorZhang, Ten_US
dc.contributor.authorYang, Zen_US
dc.contributor.authorShu, Den_US
dc.contributor.authorWang, Sen_US
dc.contributor.authorLuo, YS-
dc.date.accessioned2011-08-26T14:33:35Z-
dc.date.available2011-08-26T14:33:35Z-
dc.date.issued2011en_US
dc.identifier.citationThe 1st European Conference of Microbiology and Immunology (EUCMI2011), Budapest, Hungary, 12-14 May 2011. In European Journal of Microbiology and Immunology, 2011, v. 1 n. 2, p. 99-100en_US
dc.identifier.issn2062-509X-
dc.identifier.urihttp://hdl.handle.net/10722/137795-
dc.descriptionOral Presentation - Session 2-
dc.description.abstractBACKGROUND AND AIM: Chronic hepatitis B infection (CHB) is associated with T-cell dependent viral specific immune response. As IL-17 may constitute both early initiator of T-cell inflammatory response and essential element of cytokine network that bridges the immune system to hematopoiesis, some effects on HBV were explored in both patients and IL-17 KO mice. METHODS: Forty-two CHB patients were divided into two groups according to their HBV markers, which are Group A: HBeAg+ with normal ALT and Group B: HbsAg–, anti-HBs+. IL-17A expression level was compared after incubating the peripheral blood mononuclear cells (PBMC) with HBV antigen. Viral specific IFNγ releasing cells and TH17 phenotype were analyzed by ELISpot and FACS respectively. To explore the correlation between Th17 and anti-viral immunity, HBV mice model was created in IL-17 KO mice by hydrodynamic injection (HDI) through the tail vein with PAAV-HBV plasmid. Results: After incubation with HBcAg, IL-17 mRNA expression, Th17 and IFNγ frequencies increased significantly in Group B than in Group A (P<0.01 to 0.001). Th17/CD45RO frequency was significantly higher in Group B than in Group A. Both Th17 and IFNγ-releasing cells mostly come from memory activated T cells. In Group A, IFNγ releasing cells increased significantly and dose-dependently when the PBMCs were exposed to IL-17. IL-17 itself did not enhance PBMCs’ ability to kill AD38 and HepG2 cell lines. It has neither inhibition to HBV replication in AD38 no cytotoxicity to HepG2 growth in vitro. Three weeks after HBV-plasmid injection, HBsAg was completely clearance in wild type C57, while it was until the 3rd month after HBV HDI that all 19 IL-17 KO mice became HBsAg negative. Viral specific IFN releasing cells were much lower in IL-17 KO mice than in wild type mice during the first two weeks after HBV HDI. Anti-HBs antibody was 1000 times lower in IL-17 KO mice than in wild type mice. CONCLUSION: Anti-HBV immunity depends on Th17 as well as IL-17 expression in both CHB patients and HBV mice model at the early stage of infection. Low expression in IL-17 might be one of important factors associated with chronic HBV infection.-
dc.languageengen_US
dc.publisherAkademiai Kiado Rt.. The Journal's web site is located at http://akkrt.hu/73/journals/products/medicine/european_journal_of_microbiology_and_immunology-
dc.relation.ispartofEuropean Journal of Microbiology and Immunologyen_US
dc.rightsThe file is not the final published version of the paper. AND quote the correct citation and enclose a link to the published paper: http://dx.doi.org/ DOI of the Article.-
dc.subjectIL-17-
dc.subjectHBV infection-
dc.subjectAnimal model-
dc.titleLow TH17 expression associaton with chronic hepatitis B viral infection by means of reducing viral specific IFNγ releasing in a HBV infection model of IL-17 KO miceen_US
dc.typeConference_Paperen_US
dc.identifier.emailZhang, H: hying64@hku.hken_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1556/EuJMI.1.2011.2.1-
dc.identifier.hkuros190880en_US
dc.identifier.volume1-
dc.identifier.issue2-
dc.identifier.spage99-
dc.identifier.epage100-
dc.publisher.placeHungary-
dc.description.otherThe 1st European Conference of Microbiology and Immunology (EUCMI2011), Budapest, Hungary, 12-14 May 2011. In European Journal of Microbiology and Immunology, 2011, v. 1 n. 2, p. 99-100-

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