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Conference Paper: Effects of cigarette smoke exposure on serum 5-HT levels in rats

TitleEffects of cigarette smoke exposure on serum 5-HT levels in rats
Authors
KeywordsMedical sciences
Respiratory diseases
Issue Date2011
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
The 2011 International Conference of the American Thoracic Society (ATS), Denver, CO., 13-18 May 2011. In American Journal of Respiratory and Critical Care Medicine, 2011, v. 183 (Meeting Abstracts), abstract no. A2454 How to Cite?
AbstractRATIONALE: Chronic obstructive pulmonary disease (COPD) is characterized by airway limitation together with abnormal inflammation in the lung. One of the major causes of this disease is cigarette smoke (CS). Increased systemic serotonin (5-HT) level in non-smokers after inhaling CS (Clin Investig 1992;70:201-204) and the association of the genetic polymorphism of 5-HT transporter with pulmonary hypertension in COPD (Respiration 2010; 79:288-295), point towards a role of serotonin in COPD. This study was to investigate circulating 5-HT levels in comparison to levels of oxidative stress in an in vivo experimental rat model after CS exposure. METHODS: Sprague-Dawley rats were randomly divided into 2 groups, i.e. sham air (SA) and 4% CS for 56 days. Exposure to SA or 4% CS was performed for 1h/day in the ventilated smoking chamber. The animals were sacrificed 24h after last exposure. Serum was collected and analyzed for 5-HT levels by HPLC, markers of oxidative stress such as 8-isoprostane by ELISA and advanced oxidation protein product (AOPP) by kinetic assay. RESULTS: CS exposure elevated serum 5-HT (5.4 ± 1.5 μg/ml versus 10.6 ± 1.3 μg/ml, p = 0.03) and serum 8-isoprostane (507.9 ± 98.4 pg/ml versus 940.8 ± 92.5 pg/ml, p = 0.02) in comparison to SA exposure. In contrast, serum AOPP level was significantly lower in CS-exposed group compared to SA-exposed group (28.2 ± 3.9 μM versus 20.8 ± 2.7 μM, p = 0.04). Conclusion: We revealed that smoking exposure was associated with high levels of 5-HT and 8-isoprostane but low level of AOPP. The present finding may provide evidence of the importance of a causal relationship between smoking and 5-HT concentrations. Further studies will be required to elucidate the mechanism of CS on how 5-HT interfere oxidative stress in circulation and in lung.
DescriptionPoster Discussion Session - A108 How Useful Are Animal Models Of Lung Disease?
Persistent Identifierhttp://hdl.handle.net/10722/137793
ISSN
2015 Impact Factor: 13.118
2015 SCImago Journal Rankings: 5.832

 

DC FieldValueLanguage
dc.contributor.authorLau, KWen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorYeung, SCen_US
dc.contributor.authorIp, MSMen_US
dc.contributor.authorMak, JCWen_US
dc.date.accessioned2011-08-26T14:33:27Z-
dc.date.available2011-08-26T14:33:27Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 International Conference of the American Thoracic Society (ATS), Denver, CO., 13-18 May 2011. In American Journal of Respiratory and Critical Care Medicine, 2011, v. 183 (Meeting Abstracts), abstract no. A2454en_US
dc.identifier.issn1073-449X-
dc.identifier.urihttp://hdl.handle.net/10722/137793-
dc.descriptionPoster Discussion Session - A108 How Useful Are Animal Models Of Lung Disease?-
dc.description.abstractRATIONALE: Chronic obstructive pulmonary disease (COPD) is characterized by airway limitation together with abnormal inflammation in the lung. One of the major causes of this disease is cigarette smoke (CS). Increased systemic serotonin (5-HT) level in non-smokers after inhaling CS (Clin Investig 1992;70:201-204) and the association of the genetic polymorphism of 5-HT transporter with pulmonary hypertension in COPD (Respiration 2010; 79:288-295), point towards a role of serotonin in COPD. This study was to investigate circulating 5-HT levels in comparison to levels of oxidative stress in an in vivo experimental rat model after CS exposure. METHODS: Sprague-Dawley rats were randomly divided into 2 groups, i.e. sham air (SA) and 4% CS for 56 days. Exposure to SA or 4% CS was performed for 1h/day in the ventilated smoking chamber. The animals were sacrificed 24h after last exposure. Serum was collected and analyzed for 5-HT levels by HPLC, markers of oxidative stress such as 8-isoprostane by ELISA and advanced oxidation protein product (AOPP) by kinetic assay. RESULTS: CS exposure elevated serum 5-HT (5.4 ± 1.5 μg/ml versus 10.6 ± 1.3 μg/ml, p = 0.03) and serum 8-isoprostane (507.9 ± 98.4 pg/ml versus 940.8 ± 92.5 pg/ml, p = 0.02) in comparison to SA exposure. In contrast, serum AOPP level was significantly lower in CS-exposed group compared to SA-exposed group (28.2 ± 3.9 μM versus 20.8 ± 2.7 μM, p = 0.04). Conclusion: We revealed that smoking exposure was associated with high levels of 5-HT and 8-isoprostane but low level of AOPP. The present finding may provide evidence of the importance of a causal relationship between smoking and 5-HT concentrations. Further studies will be required to elucidate the mechanism of CS on how 5-HT interfere oxidative stress in circulation and in lung.-
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org-
dc.relation.ispartofAmerican Journal of Respiratory and Critical Care Medicineen_US
dc.subjectMedical sciences-
dc.subjectRespiratory diseases-
dc.titleEffects of cigarette smoke exposure on serum 5-HT levels in ratsen_US
dc.typeConference_Paperen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1073-449X&volume=183&issue=Meeting Abstracts&spage=abstract no. A2454&epage=&date=2011&atitle=Effects+of+cigarette+smoke+exposure+on+serum+5-HT+levels+in+rats-
dc.identifier.emailYeung, SC: flag@hkucc.hku.hken_US
dc.identifier.emailIp, MSM: msmip@hku.hken_US
dc.identifier.emailMak, JCW: judithmak@hku.hken_US
dc.identifier.authorityIp, MSM=rp00347en_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.identifier.hkuros191800en_US
dc.identifier.volume183en_US
dc.identifier.issueMeeting Abstracts-
dc.description.otherThe 2011 International Conference of the American Thoracic Society (ATS), Denver, CO., 13-18 May 2011. In American Journal of Respiratory and Critical Care Medicine, 2011, v. 183 (Meeting Abstracts), abstract no. A2454-

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