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Article: Role of polymorphonuclear neutrophils in the clearance of enterococcus faecalis derived from saliva and infected root canals

TitleRole of polymorphonuclear neutrophils in the clearance of enterococcus faecalis derived from saliva and infected root canals
Authors
KeywordsEnterococcus faecalis
immunostaining
inflammatory cytokines
polymorphonuclear neutrophils
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com
Citation
Journal Of Endodontics, 2011, v. 37 n. 3, p. 346-352 How to Cite?
AbstractObjectives: The goal of this study was to measure (1) the ability of polymorphonuclear neutrophil leukocytes (PMNs) to kill oral Enterococcus faecalis strains, (2) up-regulation of inflammatory mediators by PMNs in interaction with E. faecalis, and (3) the ability of E. faecalis to cause inflammation in mouse muscle tissue. Methods: Fifteen endodontic and nine saliva strains of E. faecalis were isolated and identified by specific 16S ribosomal RNA (16S rRNA) primers. The bacteria were grown in BHI broth and incubated with mouse PMN in appropriate media to determine the ability of the PMNs to kill the bacteria. In other experiments up-regulation of interleukin (IL)-1α, tumor necrosis factor α (TNF-α), matrix metalloproteinase-8 (MMP-8), and cyclooxygenase (COX)-2 messenger RNA in the PMNs was measured after exposure of the leukocytes to the bacteria using real-time polymerase chain reaction. Finally, the inflammatory potential of and PMN response to E. faecalis suspension in mouse muscle tissue was examined from histological sections using hematoxylin-eosin staining and immunostaining. Results: Murine PMNs killed about 80% of the E. faecalis cells in 1 hour, irrespective of the source of isolation of the strains. Quantitative PCR results showed that IL-1α, TNF-α, MMP-8, and COX-2 messenger RNA were markedly up-regulated in E. faecalis-stimulated PMNs or in E. faecalis-invaded muscular tissues. MMP-8 messenger RNA level was positively related to COX-2 messenger RNA level. Histological evaluation and immunostaining disclosed that all E. faecalis strains could recruit PMNs to the local infectious sites and cause abscess formation. Conclusion: E. faecalis strains from saliva and infected root canals have the potential to recruit PMNs in the infectious sites leading to inflammation via up-regulation of PMN IL-1α, TNF-α, MMP-8, and COX-2. PMNs can play an important role in killing of E. faecalis. Copyright © 2011 American Association of Endodontists.
Persistent Identifierhttp://hdl.handle.net/10722/137663
ISSN
2015 Impact Factor: 2.904
2015 SCImago Journal Rankings: 1.681
ISI Accession Number ID
Funding AgencyGrant Number
China International Science and Technology Cooperation Grant2009DFA32950
Funding Information:

Supported by the China International Science and Technology Cooperation Grant 2009DFA32950.

References

 

DC FieldValueLanguage
dc.contributor.authorMa, Zen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorZhu, Xen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorShen, Yen_HK
dc.contributor.authorHaapasalo, Men_HK
dc.date.accessioned2011-08-26T14:30:49Z-
dc.date.available2011-08-26T14:30:49Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Endodontics, 2011, v. 37 n. 3, p. 346-352en_HK
dc.identifier.issn0099-2399en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137663-
dc.description.abstractObjectives: The goal of this study was to measure (1) the ability of polymorphonuclear neutrophil leukocytes (PMNs) to kill oral Enterococcus faecalis strains, (2) up-regulation of inflammatory mediators by PMNs in interaction with E. faecalis, and (3) the ability of E. faecalis to cause inflammation in mouse muscle tissue. Methods: Fifteen endodontic and nine saliva strains of E. faecalis were isolated and identified by specific 16S ribosomal RNA (16S rRNA) primers. The bacteria were grown in BHI broth and incubated with mouse PMN in appropriate media to determine the ability of the PMNs to kill the bacteria. In other experiments up-regulation of interleukin (IL)-1α, tumor necrosis factor α (TNF-α), matrix metalloproteinase-8 (MMP-8), and cyclooxygenase (COX)-2 messenger RNA in the PMNs was measured after exposure of the leukocytes to the bacteria using real-time polymerase chain reaction. Finally, the inflammatory potential of and PMN response to E. faecalis suspension in mouse muscle tissue was examined from histological sections using hematoxylin-eosin staining and immunostaining. Results: Murine PMNs killed about 80% of the E. faecalis cells in 1 hour, irrespective of the source of isolation of the strains. Quantitative PCR results showed that IL-1α, TNF-α, MMP-8, and COX-2 messenger RNA were markedly up-regulated in E. faecalis-stimulated PMNs or in E. faecalis-invaded muscular tissues. MMP-8 messenger RNA level was positively related to COX-2 messenger RNA level. Histological evaluation and immunostaining disclosed that all E. faecalis strains could recruit PMNs to the local infectious sites and cause abscess formation. Conclusion: E. faecalis strains from saliva and infected root canals have the potential to recruit PMNs in the infectious sites leading to inflammation via up-regulation of PMN IL-1α, TNF-α, MMP-8, and COX-2. PMNs can play an important role in killing of E. faecalis. Copyright © 2011 American Association of Endodontists.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.jendodon.comen_HK
dc.relation.ispartofJournal of Endodonticsen_HK
dc.subjectEnterococcus faecalisen_HK
dc.subjectimmunostainingen_HK
dc.subjectinflammatory cytokinesen_HK
dc.subjectpolymorphonuclear neutrophilsen_HK
dc.subject.meshDental Pulp Cavity - microbiology-
dc.subject.meshDental Pulp Diseases - microbiology-
dc.subject.meshEnterococcus faecalis - classification - physiology-
dc.subject.meshNeutrophils - immunology - microbiology - physiology-
dc.subject.meshSaliva - microbiology-
dc.titleRole of polymorphonuclear neutrophils in the clearance of enterococcus faecalis derived from saliva and infected root canalsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0099-2399&volume=37&issue=3&spage=346&epage=352&date=2011&atitle=Role+of+Polymorphonuclear+Neutrophils+in+the+clearance+of+Enterococcus+faecalis+derived+from+saliva+and+infected+root+canals-
dc.identifier.emailZhang, C:zhangcf@hku.hken_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityZhang, C=rp01408en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.joen.2010.11.033en_HK
dc.identifier.pmid21329819-
dc.identifier.scopuseid_2-s2.0-79951614048en_HK
dc.identifier.hkuros184554en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79951614048&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue3en_HK
dc.identifier.spage346en_HK
dc.identifier.epage352en_HK
dc.identifier.eissn1878-3554-
dc.identifier.isiWOS:000288290000009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMa, Z=42661769300en_HK
dc.identifier.scopusauthoridWang, Y=35847885800en_HK
dc.identifier.scopusauthoridZhu, X=36659978300en_HK
dc.identifier.scopusauthoridZhang, C=7405494609en_HK
dc.identifier.scopusauthoridLi, S=42661946100en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridShen, Y=35574675000en_HK
dc.identifier.scopusauthoridHaapasalo, M=7003569249en_HK

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