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Article: Mucosal immunization induces a higher level of lasting neutralizing antibody response in mice by a replication-competent smallpox vaccine: Vaccinia Tiantan strain

TitleMucosal immunization induces a higher level of lasting neutralizing antibody response in mice by a replication-competent smallpox vaccine: Vaccinia Tiantan strain
Authors
Issue Date2011
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html
Citation
Journal Of Biomedicine And Biotechnology, 2011, v. 2011 How to Cite?
AbstractThe possible bioterrorism threat using the variola virus, the causative agent of smallpox, has promoted us to further investigate the immunogenicity profiles of existing vaccines. Here, we study for the first time the immunogenicity profile of a replication-competent smallpox vaccine (vaccinia Tiantan, VTT strain) for inducing neutralizing antibodies (Nabs) through mucosal vaccination, which is noninvasive and has a critical implication for massive vaccination programs. Four different routes of vaccination were tested in parallel including intramuscular (i.m.), intranasal (i.n.), oral (i.o.), and subcutaneous (s.c.) inoculations in mice. We found that one time vaccination with an optimal dose of VTT was able to induce anti-VTT Nabs via each of the four routes. Higher levels of antiviral Nabs, however, were induced via the i.n. and i.o. inoculations when compared with the i.m. and s.c. routes. Moreover, the i.n. and i.o. vaccinations also induced higher sustained levels of Nabs overtime, which conferred better protections against homologous or alternating mucosal routes of viral challenges six months post vaccination. The VTT-induced immunity via all four routes, however, was partially effective against the intramuscular viral challenge. Our data have implications for understanding the potential application of mucosal smallpox vaccination and for developing VTT-based vaccines to overcome preexisting antivaccinia immunity. Copyright © 2011 Bin Lu et al.
Persistent Identifierhttp://hdl.handle.net/10722/137593
ISSN
2014 Impact Factor: 3.169
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Chinese 973 project2006CB504208
11th 5-year project2008ZX10001-011
2008ZX10001-015
Hong Kong research grant councilHK-RGC762209
University of Hong Kong
Funding Information:

This work was supported by the Chinese 973 project 2006CB504208, the 11th 5-year project 2008ZX10001-011 and 2008ZX10001-015, Hong Kong research grant council (HK-RGC762209 to ZC), and the UDF/LKS grants of the University of Hong Kong to its AIDS Institute. We thank Jenny Ng for editorial inputs. B. Lu, W. Yu, and X. Huang contributed equally to this work.

References

 

DC FieldValueLanguage
dc.contributor.authorChen, Zen_HK
dc.contributor.authorLu, Ben_HK
dc.contributor.authorYu, Wen_HK
dc.contributor.authorHuang, Xen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorLiu, Len_HK
dc.date.accessioned2011-08-26T14:28:24Z-
dc.date.available2011-08-26T14:28:24Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Biomedicine And Biotechnology, 2011, v. 2011en_HK
dc.identifier.issn1110-7243en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137593-
dc.description.abstractThe possible bioterrorism threat using the variola virus, the causative agent of smallpox, has promoted us to further investigate the immunogenicity profiles of existing vaccines. Here, we study for the first time the immunogenicity profile of a replication-competent smallpox vaccine (vaccinia Tiantan, VTT strain) for inducing neutralizing antibodies (Nabs) through mucosal vaccination, which is noninvasive and has a critical implication for massive vaccination programs. Four different routes of vaccination were tested in parallel including intramuscular (i.m.), intranasal (i.n.), oral (i.o.), and subcutaneous (s.c.) inoculations in mice. We found that one time vaccination with an optimal dose of VTT was able to induce anti-VTT Nabs via each of the four routes. Higher levels of antiviral Nabs, however, were induced via the i.n. and i.o. inoculations when compared with the i.m. and s.c. routes. Moreover, the i.n. and i.o. vaccinations also induced higher sustained levels of Nabs overtime, which conferred better protections against homologous or alternating mucosal routes of viral challenges six months post vaccination. The VTT-induced immunity via all four routes, however, was partially effective against the intramuscular viral challenge. Our data have implications for understanding the potential application of mucosal smallpox vaccination and for developing VTT-based vaccines to overcome preexisting antivaccinia immunity. Copyright © 2011 Bin Lu et al.en_HK
dc.languageengen_US
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.htmlen_HK
dc.relation.ispartofJournal of Biomedicine and Biotechnologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntibodies, Neutralizing - genetics - immunology-
dc.subject.meshImmunity, Mucosal - immunology-
dc.subject.meshSmallpox Vaccine - administration and dosage - immunology-
dc.subject.meshVaccinia - immunology - prevention and control-
dc.subject.meshVaccinia virus - genetics - immunology-
dc.titleMucosal immunization induces a higher level of lasting neutralizing antibody response in mice by a replication-competent smallpox vaccine: Vaccinia Tiantan strainen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1110-7243&volume=2011, article no. 970424&spage=&epage=&date=2011&atitle=Mucosal+immunization+induces+a+higher+level+of+lasting+neutralizing+antibody+response+in+mice+by+a+replication-competent+smallpox+vaccine:+vaccinia+Tiantan+strain-
dc.identifier.emailChen, Z: zchenai@hku.hken_HK
dc.identifier.emailWang, H: haibo@hku.hken_HK
dc.identifier.emailLiu, L: liuli71@hkucc.hku.hken_HK
dc.identifier.authorityChen, Z=rp00243en_HK
dc.identifier.authorityWang, H=rp00279en_HK
dc.identifier.authorityLiu, L=rp00268en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2011/970424en_HK
dc.identifier.pmid21765641-
dc.identifier.pmcidPMC3134386-
dc.identifier.scopuseid_2-s2.0-80052674064en_HK
dc.identifier.hkuros190679en_US
dc.identifier.hkuros206298en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052674064&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2011en_HK
dc.identifier.isiWOS:000293554800001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, Z=35271180800en_HK
dc.identifier.scopusauthoridLu, B=50162127500en_HK
dc.identifier.scopusauthoridYu, W=50162901500en_HK
dc.identifier.scopusauthoridHuang, X=22234542700en_HK
dc.identifier.scopusauthoridWang, H=36143443600en_HK
dc.identifier.scopusauthoridLiu, L=35784425200en_HK

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