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Article: Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands

TitleTransplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands
Authors
Issue Date2011
PublisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2011, v. 124 n. 15, p. 2260-2268 How to Cite?
AbstractBACKGROUND: Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection. METHODS: The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis. RESULTS: Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P < 0.05). CONCLUSIONS: Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages.
Persistent Identifierhttp://hdl.handle.net/10722/137499
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
Funding AgencyGrant Number
National Basic Science and Development Program of China (973 Program)2005CB522603
National Natural Science Foundation of China81000011
81000835
Distinguished Young Talents in Higher Education of GuangdongLYM091182009
Shenzhen Technological RD FoundationJC201005280429A
Funding Information:

This work was supported by grants from the National Basic Science and Development Program of China (973 Program, No. 2005CB522603) and National Natural Science Foundation of China (No. 81000011, 81000835), Distinguished Young Talents in Higher Education of Guangdong (No. LYM091182009), and Shenzhen Technological R&D Foundation (No. JC201005280429A).

 

DC FieldValueLanguage
dc.contributor.authorCai, Sen_US
dc.contributor.authorPan, Yen_US
dc.contributor.authorHan, Ben_US
dc.contributor.authorSun, TZen_US
dc.contributor.authorSheng, ZYen_US
dc.contributor.authorFu, XBen_US
dc.date.accessioned2011-08-26T14:26:29Z-
dc.date.available2011-08-26T14:26:29Z-
dc.date.issued2011en_US
dc.identifier.citationChinese Medical Journal, 2011, v. 124 n. 15, p. 2260-2268en_US
dc.identifier.issn0366-6999-
dc.identifier.urihttp://hdl.handle.net/10722/137499-
dc.description.abstractBACKGROUND: Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection. METHODS: The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis. RESULTS: Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P < 0.05). CONCLUSIONS: Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages.-
dc.languageengen_US
dc.publisherZhonghua Yixuehui. The Journal's web site is located at http://www.cmj.org/-
dc.relation.ispartofChinese Medical Journalen_US
dc.subject.meshBone Marrow Cells - cytology-
dc.subject.meshEctodysplasins - genetics - metabolism-
dc.subject.meshMesenchymal Stem Cell Transplantation - methods-
dc.subject.meshMesenchymal Stem Cells - cytology - metabolism-
dc.subject.meshSweat Glands - cytology - metabolism-
dc.titleTransplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glandsen_US
dc.typeArticleen_US
dc.identifier.emailCai, S: caisa@hku.hken_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3760/cma.j.issn.0366-6999.2011.15.004-
dc.identifier.pmid21933554-
dc.identifier.scopuseid_2-s2.0-79961075103-
dc.identifier.hkuros191420en_US
dc.identifier.volume124en_US
dc.identifier.issue15-
dc.identifier.spage2260en_US
dc.identifier.epage2268en_US
dc.identifier.isiWOS:000294584100004-
dc.publisher.placeChina-

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