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Article: Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide

TitleDifferential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide
Authors
KeywordsAdductor muscle
Cadmium
ER stress
Hepatopancreas
Hydrogen peroxide
Mussel
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2011, v. 105 n. 1-2, p. 49-61 How to Cite?
AbstractThis study aimed to reveal the proteomic responses in the hepatopancreas and adductor muscle of a common biomonitor, Perna viridis after 14-day exposure to two model chemicals, cadmium (Cd; a toxic metal) and hydrogen peroxide (H 2O 2; a pro-oxidant), using two-dimensional gel electrophoresis coupled with multivariate statistical analyses. Unique sets of tissue-specific protein expression signatures were revealed corresponding to the two treatment groups. In the hepatopancreas, 15 and 2 spots responded to Cd and H 2O 2 treatments respectively. 6 and 7 spots were differentially expressed in the adductor muscle for Cd and H 2O 2 treatments, respectively. 15 differentially expressed spots were successfully identified by MALDI-TOF/TOF MS analysis. These proteins are involved in glycolysis, amino acid metabolism, energy homeostasis, oxidative stress response, redox homeostasis and protein folding, heat-shock response, and muscle contraction modulation. This is the first time, to have demonstrated that Cd exposure not only leads to substantial oxidative stress but also results in endoplasmic reticulum stress in hepatopancreas of the mussel. Such notable stress responses may be attributable to high Cd accumulation in this tissue. Our results suggested that investigations on these stress-associated protein changes could be used as a new and complementary approach in pollution monitoring by this popular biomonitor species. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/137491
ISSN
2015 Impact Factor: 3.557
2015 SCImago Journal Rankings: 1.671
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region GovernmentHKU7522/06M
University of Hong Kong
Funding Information:

This project is supported by the Research Grants Council of the Hong Kong Special Administrative Region Government via a General Research Fund (project no. HKU7522/06M, KMY Leung) and the Seed Funding for Basic Research by the University of Hong Kong (KMY Leung). We thank Ms. Ivy Law and Ms. Ava Zhou (Department of Pharmacology, HKU) for their assistance in western blotting; Ms. Carol Lau, Mr. Lawrence Luk, Mr. jack Man and Mr. Ben Ho (Genome Research Centre, HKU) for helping with the proteomic sample processing. We also thank Dr. Tak-Cheung Wai (The Swire Institute of Marine Science, HKU) for his advice on multivariate statistical analyses, and Dr. Vengatesen Thiyagarajan and Mr. Nico Zurcher (School of Biological Sciences, HKU) for their useful comments on early drafts of this manuscript; Dr. Stephen R. Cartwright for improving the English of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorLeung, PTYen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorMak, SSTen_HK
dc.contributor.authorNg, WCen_HK
dc.contributor.authorLeung, KMYen_HK
dc.date.accessioned2011-08-26T14:26:08Z-
dc.date.available2011-08-26T14:26:08Z-
dc.date.issued2011en_HK
dc.identifier.citationAquatic Toxicology, 2011, v. 105 n. 1-2, p. 49-61en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/137491-
dc.description.abstractThis study aimed to reveal the proteomic responses in the hepatopancreas and adductor muscle of a common biomonitor, Perna viridis after 14-day exposure to two model chemicals, cadmium (Cd; a toxic metal) and hydrogen peroxide (H 2O 2; a pro-oxidant), using two-dimensional gel electrophoresis coupled with multivariate statistical analyses. Unique sets of tissue-specific protein expression signatures were revealed corresponding to the two treatment groups. In the hepatopancreas, 15 and 2 spots responded to Cd and H 2O 2 treatments respectively. 6 and 7 spots were differentially expressed in the adductor muscle for Cd and H 2O 2 treatments, respectively. 15 differentially expressed spots were successfully identified by MALDI-TOF/TOF MS analysis. These proteins are involved in glycolysis, amino acid metabolism, energy homeostasis, oxidative stress response, redox homeostasis and protein folding, heat-shock response, and muscle contraction modulation. This is the first time, to have demonstrated that Cd exposure not only leads to substantial oxidative stress but also results in endoplasmic reticulum stress in hepatopancreas of the mussel. Such notable stress responses may be attributable to high Cd accumulation in this tissue. Our results suggested that investigations on these stress-associated protein changes could be used as a new and complementary approach in pollution monitoring by this popular biomonitor species. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectAdductor muscleen_HK
dc.subjectCadmiumen_HK
dc.subjectER stressen_HK
dc.subjectHepatopancreasen_HK
dc.subjectHydrogen peroxideen_HK
dc.subjectMusselen_HK
dc.titleDifferential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxideen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-445X&volume=105&spage=49&epage=61&date=2011&atitle=Differential+proteomic+responses+in+hepatopancreas+and+adductor+muscles+of+the+green-lipped+mussel+Perna+viridis+to+stresses+induced+by+cadmium+and+hydrogen+peroxide-
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityLeung, KMY=rp00733en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2011.05.010en_HK
dc.identifier.pmid21684241en_HK
dc.identifier.scopuseid_2-s2.0-79960328344en_HK
dc.identifier.hkuros190377en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960328344&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume105en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage49en_HK
dc.identifier.epage61en_HK
dc.identifier.isiWOS:000294317500006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLeung, PTY=35740926800en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridMak, SST=54404915400en_HK
dc.identifier.scopusauthoridNg, WC=24723024100en_HK
dc.identifier.scopusauthoridLeung, KMY=7401860738en_HK
dc.identifier.citeulike9372691-

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