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Article: Factors affecting mesenchymal stromal cells yield from bone marrow aspiration
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TitleFactors affecting mesenchymal stromal cells yield from bone marrow aspiration
 
AuthorsLi, J2 1
Wong, WHS1
Chan, S1
Chim, JCS1
Cheung, KMC1
Lee, TL1
Au, WY1
Ha, SY1
Lie, AKW1
Lau, YL1
Liang, RHS1
Chan, GCF1
 
KeywordsBone marrow aspiration
CFU-F
Leukemia
Mesenchymal stromal cell
 
Issue Date2011
 
PublisherChina Anti-Cancer Association. The Journal's web site is located at http://zgazyj.periodicals.net.cn/
 
CitationChinese Journal Of Cancer Research, 2011, v. 23 n. 1, p. 43-48 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s11670-011-0043-1
 
AbstractObjective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC). Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles. Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle. Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion. © 2011 Chinese Anti-Cancer Association and Springer.
 
ISSN1000-9604
2012 Impact Factor: 0.448
2012 SCImago Journal Rankings: 0.191
 
DOIhttp://dx.doi.org/10.1007/s11670-011-0043-1
 
ISI Accession Number IDWOS:000290804200009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, J
 
dc.contributor.authorWong, WHS
 
dc.contributor.authorChan, S
 
dc.contributor.authorChim, JCS
 
dc.contributor.authorCheung, KMC
 
dc.contributor.authorLee, TL
 
dc.contributor.authorAu, WY
 
dc.contributor.authorHa, SY
 
dc.contributor.authorLie, AKW
 
dc.contributor.authorLau, YL
 
dc.contributor.authorLiang, RHS
 
dc.contributor.authorChan, GCF
 
dc.date.accessioned2011-08-26T14:25:50Z
 
dc.date.available2011-08-26T14:25:50Z
 
dc.date.issued2011
 
dc.description.abstractObjective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC). Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles. Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle. Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion. © 2011 Chinese Anti-Cancer Association and Springer.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationChinese Journal Of Cancer Research, 2011, v. 23 n. 1, p. 43-48 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s11670-011-0043-1
 
dc.identifier.citeulike9004087
 
dc.identifier.doihttp://dx.doi.org/10.1007/s11670-011-0043-1
 
dc.identifier.epage48
 
dc.identifier.hkuros191919
 
dc.identifier.isiWOS:000290804200009
 
dc.identifier.issn1000-9604
2012 Impact Factor: 0.448
2012 SCImago Journal Rankings: 0.191
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-79953210080
 
dc.identifier.spage43
 
dc.identifier.urihttp://hdl.handle.net/10722/137476
 
dc.identifier.volume23
 
dc.languageeng
 
dc.publisherChina Anti-Cancer Association. The Journal's web site is located at http://zgazyj.periodicals.net.cn/
 
dc.publisher.placeChina
 
dc.relation.ispartofChinese Journal of Cancer Research
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBone marrow aspiration
 
dc.subjectCFU-F
 
dc.subjectLeukemia
 
dc.subjectMesenchymal stromal cell
 
dc.titleFactors affecting mesenchymal stromal cells yield from bone marrow aspiration
 
dc.typeArticle
 
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<contributor.author>Wong, WHS</contributor.author>
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<contributor.author>Chim, JCS</contributor.author>
<contributor.author>Cheung, KMC</contributor.author>
<contributor.author>Lee, TL</contributor.author>
<contributor.author>Au, WY</contributor.author>
<contributor.author>Ha, SY</contributor.author>
<contributor.author>Lie, AKW</contributor.author>
<contributor.author>Lau, YL</contributor.author>
<contributor.author>Liang, RHS</contributor.author>
<contributor.author>Chan, GCF</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Fuwai Hospital