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Article: Effects of tanshinone IIA, a major component of Salvia miltiorrhiza, on platelet aggregation in healthy newborn piglets

TitleEffects of tanshinone IIA, a major component of Salvia miltiorrhiza, on platelet aggregation in healthy newborn piglets
Authors
KeywordsDanshen (Salvia miltiorrhiza)
Endothelial microparticles
Newborn piglet
Platelet aggregation
Tanshinone IIA
Thromboxane B 2
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 44-49 How to Cite?
AbstractEthnopharmacological relevance: Tanshinone IIA (STS), an active ingredient of the Chinese herb Danshen (Salvia miltiorrhiza) for angina and stroke in adults, has been reported to inhibit platelet function. However, its effect on platelet and underlying mechanism remain largely unknown, particularly in neonates. Materials and Methods: To investigate the effect of STS on the platelet aggregation and its interaction with various platelet activation pathways, platelet aggregatory function was studied in whole blood stimulated by collagen (2-10 μg/ml) ex vivo in newborn piglets receiving intravenous STS (0.1-10 mg/kg, n = 8) and in vitro in whole blood from newborn piglets (n = 6) incubated with STS (0.1-100 μg/ml). The respective morphological changes of platelets were also examined by scanning electron microscopy. Plasma levels of nitrite/nitrate (NOx) and thromboxane B 2 (TxB 2), matrix metalloproteinase (MMP)-2 and -9 activities were also examined. To further delineate the mechanistic pathway, the effect of STS on endothelial microparticles release from cultured human umbilical vein endothelial cells (HUVECs) was quantified by flow cytometry. Results: STS impaired the ex vivo, but not in vitro, collagen-stimulated platelet aggregation. Infusion of STS elevated the plasma level of TxB 2 at 10 mg/kg. However, STS had no effect on NOx level. Incubating cultured HUVECs with STS (1 and 10 μg/ml) caused a significant release of endothelial microparticles. Morphologically, STS elicited platelet activation in vivo, but not in vitro. Conclusions: STS impairs the ex vivo whole blood platelet aggregatory function by activating platelet in vivo in healthy newborn piglets. It implies that STS may elicit its effects by stimulating endothelial microparticles production and eicosanoid metabolism pathway. © 2011 Elsevier Ireland Ltd All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/137475
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
Funding AgencyGrant Number
Canadian Institutes of Health ResearchMOP51306
Alberta Heritage Foundation for Medical Research
Funding Information:

The authors sincerely express their appreciation to Judy Li, for her excellent technical assistance. This study was supported by the operating grants from the Canadian Institutes of Health Research (MOP51306) and Alberta Heritage Foundation for Medical Research.

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, JQen_HK
dc.contributor.authorLee, TFen_HK
dc.contributor.authorMiedzyblocki, Men_HK
dc.contributor.authorChan, GCFen_HK
dc.contributor.authorBigam, DLen_HK
dc.contributor.authorCheung, PYen_HK
dc.date.accessioned2011-08-26T14:25:46Z-
dc.date.available2011-08-26T14:25:46Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2011, v. 137 n. 1, p. 44-49en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137475-
dc.description.abstractEthnopharmacological relevance: Tanshinone IIA (STS), an active ingredient of the Chinese herb Danshen (Salvia miltiorrhiza) for angina and stroke in adults, has been reported to inhibit platelet function. However, its effect on platelet and underlying mechanism remain largely unknown, particularly in neonates. Materials and Methods: To investigate the effect of STS on the platelet aggregation and its interaction with various platelet activation pathways, platelet aggregatory function was studied in whole blood stimulated by collagen (2-10 μg/ml) ex vivo in newborn piglets receiving intravenous STS (0.1-10 mg/kg, n = 8) and in vitro in whole blood from newborn piglets (n = 6) incubated with STS (0.1-100 μg/ml). The respective morphological changes of platelets were also examined by scanning electron microscopy. Plasma levels of nitrite/nitrate (NOx) and thromboxane B 2 (TxB 2), matrix metalloproteinase (MMP)-2 and -9 activities were also examined. To further delineate the mechanistic pathway, the effect of STS on endothelial microparticles release from cultured human umbilical vein endothelial cells (HUVECs) was quantified by flow cytometry. Results: STS impaired the ex vivo, but not in vitro, collagen-stimulated platelet aggregation. Infusion of STS elevated the plasma level of TxB 2 at 10 mg/kg. However, STS had no effect on NOx level. Incubating cultured HUVECs with STS (1 and 10 μg/ml) caused a significant release of endothelial microparticles. Morphologically, STS elicited platelet activation in vivo, but not in vitro. Conclusions: STS impairs the ex vivo whole blood platelet aggregatory function by activating platelet in vivo in healthy newborn piglets. It implies that STS may elicit its effects by stimulating endothelial microparticles production and eicosanoid metabolism pathway. © 2011 Elsevier Ireland Ltd All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.subjectDanshen (Salvia miltiorrhiza)en_HK
dc.subjectEndothelial microparticlesen_HK
dc.subjectNewborn pigleten_HK
dc.subjectPlatelet aggregationen_HK
dc.subjectTanshinone IIAen_HK
dc.subjectThromboxane B 2en_HK
dc.titleEffects of tanshinone IIA, a major component of Salvia miltiorrhiza, on platelet aggregation in healthy newborn pigletsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=137&issue=1&spage=44&epage=49&date=2011&atitle=Effects+of+tanshinone+IIA,+a+major+component+of+Salvia+miltiorrhiza,+on+platelet+aggregation+in+healthy+newborn+pigletsen_US
dc.identifier.emailChan, GCF:gcfchan@hkucc.hku.hken_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2011.03.047en_HK
dc.identifier.pmid21453766-
dc.identifier.scopuseid_2-s2.0-80052031837en_HK
dc.identifier.hkuros191916en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052031837&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume137en_HK
dc.identifier.issue1en_HK
dc.identifier.spage44en_HK
dc.identifier.epage49en_HK
dc.identifier.isiWOS:000295236700005-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridLiu, JQ=50162013300en_HK
dc.identifier.scopusauthoridLee, TF=35311188100en_HK
dc.identifier.scopusauthoridMiedzyblocki, M=35076303900en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK
dc.identifier.scopusauthoridBigam, DL=6701639393en_HK
dc.identifier.scopusauthoridCheung, PY=7202595327en_HK

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